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http://dx.doi.org/10.5487/TR.2008.24.1.029

The Effects of Crinum asiaticum on the Apoptosis Induction and the Reversal of Multidrug Resistance in HL-60/MX2  

Hyun, Jae-Hee (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University)
Kang, Jung-Il (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University)
Kim, Sang-Cheol (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University)
Kim, Elvira (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University)
Kang, Ji-Hoon (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University)
Kwon, Jung-Mi (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University)
Park, Doek-Bae (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University)
Lee, Young-Jae (Department of Veterinary Medicine, College of Applied Life Science, Cheju National University)
Yoo, Eun-Sook (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University)
Kang, Hee-Kyoung (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University)
Publication Information
Toxicological Research / v.24, no.1, 2008 , pp. 29-36 More about this Journal
Abstract
The present study investigated the anti-proliferative and chemosensitizing effects of Crinum asiaticum var. japonicum against multi-drug resistant (MDR) cancer cells. The 80% methanol extract, chloroform ($CHCl_3$) fraction and butanol (BuOH) fraction of C. asiaticum inhibited the growth of mitoxantrone (MX) resistant HL-60 (HL-60/MX2) cells. When HL-60/MX2 cells were treated with the $CHCl_3$ and BuOH fractions, DNA ladder and sub-G1 hypodiploid cells were observed. Furthermore, the fractions reduced BcI-2 mRNA levels, whereas Bax mRNA levels were increased. These results suggest that the inhibitory effect of C. asiaticum on the growth of the HL-60/MX2 cells might arise from the induction of apoptosis. Treatment of HL-60/MX2 cells with the fractions markedly decreased the mRNA levels of the multi-drug resistance protein-1 and breast cancer resistance protein. The $CHCl_3$ fraction and hexane fraction increased MX accumulation in HL-60/MX2 cells. These results imply that the $CHCl_3$ fraction of C. asiaticum plays a pivotal role as a chemosensitizer. We suggest that components of C. asiaticum might have a therapeutic potential for the treatment of MDR leukemia.
Keywords
HL-60/MX2; Crinum asiaticum; Apoptosis; Chemosensitizer; MRP-1; BCRP;
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