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http://dx.doi.org/10.5487/TR.2007.23.4.347

Anti-Oxidative Effects of Rubus coreanum Miquel Extract on Hepatic Injury Induced by Lipopolysaccharide  

Kim, In-Deok (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Kang, Kum-Suk (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Kwon, Ryun-Hee (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Ha, Bae-Jin (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Publication Information
Toxicological Research / v.23, no.4, 2007 , pp. 347-352 More about this Journal
Abstract
The protective effects of Rubus coreanum Miquel (RCM) extract against LPS-induced hepatotoxicity were studied in rats. Squrague-Dawley rats were intraperitoneally administered the RCM at 100 mg/kg per day for three weeks. Then single dose of LPS (5 mg/kg) was injected into rats. Four hours later, they were anesthesized with ether and dissected. We examined the levels of glutamate oxaloacetate transaminase (AST), glutamate pyruvate transaminase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) in sera, superoxide dismutase (SOD) in mitochondrial fraction and catalase (CAT), glutathione peroxidase (GPx) in liver homogenate. LPS-treatment markedly increased the levels of AST, ALT, ALP, LDH and significantly decreased those of SOD, CAT and GPx. But RCM-pretreatment decreased the levels of AST, ALT, ALP and LDH by 57.9%, 37.4%, 62% and 69% respectively and increased those of SOD, CAT and GPx by 82.9%, 64.2% and 96.7% respectively. Subsequently, the protective effects of RCM was evaluated through histopathological examination of liver tissue. The LPS treatment increased the state of necrosis and cirrhosis surrounding the central veins (CV) and sinusoid, but RCM-treatment decreased the state of necrosis and cirrhosis in the liver tissue. These results demonstrated that protective effects of RCM against LPS-induced hepatotoxicity.
Keywords
Rubus coreanum Miquel; preventive effect; Hepatotoxicity; Lipopolysaccharide;
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1 Beauchamp, C. and Fridovich, I. (1971). Superoxide dismutase improved assays and an assay applicable to acrylamide gels. Anal. Biochem., 44, 276-287   DOI   ScienceOn
2 Borek, C. (2001). Antioxidant health effects of aged garlic extract. The Journal of Nutrition, 131, 1010-1025
3 Dantzer, R., Bluthe, R.M., Gheusi, G., Cremona, S., Laye, S., Parnet, P. and Kelley, K.W. (1998). Molecular basis of sickness behavior. Annals N. Y. Acad. Sci., 856, 126-134
4 Fridovich, I. (1986). Biologic effects of the superoxide radicals. Arch. Biochem. Biophysm., 247, 1-15   DOI   ScienceOn
5 Halliwell, B. (1990). How to charactrize a biological antioxidant. Free Radic. Res. Commun., 9, 1-12   DOI
6 Jeong, C.S., Jung, K.W. and Jeong, J.S. (1999). Hepatoprotective effect of subfractions of Carthamus tinctorius L. Semen on the reversal of biotransformation enzyme activities in $CCl_{4}$-induced hepatotoxic rats. J. Fd. Hyg. Safety, 14, 172-178
7 Kwak. J.S., Kim, K.S. and Kim, D.H. (1995). The changes of the liver tissue induced by lipopolysaccharide. The Kyungpook University Medical Journal, 36, 507-521
8 Takaharu, N. and Kiyonori, Y. (1999). Low-dose ${\gamma}$-ray irradiation reduces oxidative damage induced by $CCl_{4}$ in mouse liver. Free Radical Biology & Medicine, 27, 1324-1333   DOI   ScienceOn
9 Kuresh, A.Y. and James, A.J. (2001). A possible emerging role of phytochemicals in improving age-related neurological dysfunctions: A multiplicity of effects. Free Radical Biology. and Med., 30, 583-594   DOI   ScienceOn
10 Lee, M.W. (1995). Phenolic compounds from the leaves of Rubus coreaum. Korean J. Pharmacogn., 39, 200-204   과학기술학회마을
11 Cha, H.S., Park, K.S. and Park, K.M. (2001). Physiological activities of Rubus coreanus Miquel. Korean J. Food. Sci. Technol., 33, 409-415
12 Dan, B. and Andrew, G. (1993). Chinese herbal medicine; materia medica. Eastland Press, 8, 375-392
13 Gabriel, L.P. and Hewitt, R.W. (1982). Principles and methods of toxicology. Raven Press, 8, 407-445
14 Rosen, D.R., Jakobisiak, M., Hartmann, R.P., Ortega, S. and Johnston, D.J. (1993). Mutations with familial amyotrophic lateral sclerosis. Nature, 362, 59-62   DOI   ScienceOn
15 Tainer, J.A., Getzoff, E.D., Richardson, J.S. and Richardson, D.C. (1983). Structure and mechanism of Cu, Zn, superoxide dismutase. Nature, 306, 274-287   DOI   ScienceOn
16 Yosjikawa, T., Murakami, M., Yoshida, N., Seto, O. and Kondo, M. (1983). Effects of superoxide dimutase and catalase on disseminated intravascular coagulation in rats. Thromb. Haemostas., 50, 869-872
17 Aebi, H. (1984). Catalase in vitro methods. Enzymology, 105,121-126   DOI
18 Jean, L., Bret, D. and Chistophe, C. (1997). Systemic capsaicin pretreatment fails to block the decrease in food-motivated behavior induced by lipopolysaccharide and interleukin-1$\beta$. Brain Research Bul., 42, 443-449   DOI   ScienceOn
19 Lawrence, R.A and Burk, R.F. (1976). Glutathione peroxidase activity in selenium-deficient rat liver. Biochem. Biophys. Res. Commun., 71, 952-958   DOI   ScienceOn
20 Park, J.H., Lee, H.S., Mun, H.C., Kim, D.H., Seong, N.S., Jung, H.G., Bang, J.K. and Lee, H.Y. (2004b). Improvement of anticancer activation of ultrasonificated extracts from Acanthopanax senticosus Harms, Ephedra sinica Stapf and Rubus coreanus Miq. Korean J. Biotechnol. Bioeng., 12, 273-278   과학기술학회마을
21 Ambrogi, L.P. (1982). Manual of histological and special staining technics. Armed. Forces. Institute of Pathology, 33, 407-445
22 Lowry, O.H., Rosenbrough, N.J., Farr, A.S. and Randall, R.J. (1951): Protein measurenment with folin phenol reagent. J. Biol. Chem., 193, 256-261
23 Enomoto, N., Yamashina, S. and Kono, H. (1999). Development of a new simple rat model of early alcohol-induced liver injury based on sensitization of kupffer cells. Hepatology, 29, 1680-1689   DOI
24 Lee, Y.A. and Lee, M.W. (1995). Tannins from Rubus coreanum. Korean J. Pharmacogn., 6, 27-30
25 Kim, E.J., Lee, Y.J., Shin, H.K. and Park, J.H. (2005). Induction of apoptosis by the aqueous extract of Rubus coreanum in HT-29 human colon cancer cells. Nutrition, 21, 1141-1148   DOI   ScienceOn
26 Kim, H.C. and Lee, S.I. (1991). Comparision of the pharmacological effects of kinds of Rubi Fructus. J. Herbology, 6, 3-12
27 Park, J.H., Lee, H.S., Mun, H.C., Kim, D.H., Seong, N.S., Jung, H.G., Bang, J.K. and Lee, H.Y. (2004a). Effect of Ultrasonification process on enhanscement of immunostimulatory activity of Epherdra sinica Stapf and Rubus coreanus Miq. Korean J. Biotechnol. Bioeng., 19, 113-117
28 Yang, H.M., Lim, S.S., Lee, Y.S., Shin, H.K., Oh, Y.S. and Kim, J.K. (2007). Comparison of the Anti-inflammatory effects of the extracts from Rubus coreanus and Rubus occidentalis. Korean J. Food Sci. Technol., 39, 342-347   과학기술학회마을
29 Gutteridge, J.M.C., Beard, A.P.C. and Quinlan, G.J. (1983). Superoxide-dependent lipid peroxidation; Problem with the use of catalase as a specific probe for Fenton-derived hydroxyl radicals. Biochem. Biophys. Res. Commun., 117, 901-907   DOI   ScienceOn
30 Lee, H.Y. (2003). Screening of biological activities of the extracts from Rubus coreanus Miq. Korean J.. Medicinal Crop. Sci., 11, 5-12
31 Middleton, E. (1996). Biological properties of plant flavonoids an overview. Int. J. Pharmcognosy, 34, 344-348   DOI   ScienceOn
32 Kim, T.J. (1997). Korean resources plants. Seoul University Republished, 11, 276-285