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http://dx.doi.org/10.5487/TR.2007.23.4.331

Relative Evaluation for Biocompatibility of Pure Titanium and Titanium Alloys using Histological and Enzymatic Methods  

Yeom, Dong-Sun (Department of Material Science and Metallurgical Engineering, Sunchon National University)
Kim, Byung-Il (Department of Material Science and Metallurgical Engineering, Sunchon National University)
Lee, Yu-Mi (Department of Biology, Sunchon National University)
Lee, Eun-Jung (Department of Biology, Sunchon National University)
Yee, Sung-Tae (Department of Biology, Sunchon National University)
Seong, Chi-Nam (Department of Biology, Sunchon National University)
Seo, Kwon-Il (Department of Food Nutrition, Sunchon National University)
Cho, Hyun-Wook (Department of Biology, Sunchon National University)
Publication Information
Toxicological Research / v.23, no.4, 2007 , pp. 331-339 More about this Journal
Abstract
Titanium or titanium alloy is a widely used implant material according to its certified biocompatibility, sufficient strength and ready availability. The purpose of this study was to evaluate the relative biocompatibility of titanium and titanium alloy specimens (Ti-29Nb-13Ta, TiNb and Ti-6Al-4V, Ti64) using in vivo and in vitro methods. For in vivo experiment, the specimens were implanted in the abdominal subcutaneous region of female mice for 2 and 4 weeks. The reaction of connective tissue to specimens was evaluated histologically. The specimens were encapsulated by fibrous connective tissue consisting of fibroblast, fibrocyte and other cells including neutrophil, macrophage, giant multinucleated cell and unidentified cells. Some newly formed blood vessels were located in the fibrous capsule surrounding the implant. Cell types and the thickness of fibrous capsules were examined quantitatively. Most of cell types located in the fibrous capsule were fibroblasts and fibrocytes. The average thickness of fibrous capsules for the TiNb specimens was much thinner than that of the titanium alloy, Ti64. The thickness of the fibrous capsule around all titanium specimens decreased at 4 weeks compared to 2 weeks post-implantation. The biocompatibility of titanium and titanium alloy specimens were also investigated in in vitro method using alkaline phosphatase from MG-63 cells. Alkaline phosphatase activity of the TiNb specimen showed higher activity than the titanium alloy, Ti64. In conclusion, the TiNb alloy with thin capsule thickness in vivo and high alkaline phosphatase activity in vitro will be of considerable use in biomedical applications.
Keywords
Biocompatibility; Titanium alloy; Abdominal connective tissue; MG-63 cell;
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1 DeVoto, E. and Yokel, R.A. (1994). The biological speciation and toxicokinetics of aluminum. Environ. Health Perspect., 102, 940-951   DOI
2 Johnson, R., Harrison, D., Tucci, M., Tsao, A., Lemos, M., Puckett, A., Hughes, J.L. and Benghuzzi, H. (1997). Fibrous capsule formation in response to ultra high molecular weight polyethylene treated with peptides that influence adhesion. Biomed. Sci. Instrum., 34, 47-52
3 Rogers, S.D., Howie, D.W., Graves, S.E., Pearcy, M.J. and Haynes, D.R. (1997). In vitro human monocyte response to wear particles of titanium alloy containing vanadium or niobium. J. Bone Joint Surg. Br., 79, 311-315   DOI
4 Ryhnen, J., Kallioinen, M., Tuukkanen, J., Junila, J., Niemel, E., Sandvik, P. and Serlo, W. (1998). In vivo biocompatibility evaluation of nickel-titanium shape memory metal alloy: muscle and perineural tissue responses and encapsule membrane thickness. J. Biomed. Mater. Res., 41, 481-488   DOI   ScienceOn
5 Salthouse, T.N. (1984). Some aspects of macrophage behavior at the implant interface. J. Biomed. Mater. Res., 18, 395-401   DOI
6 Schreiber, H., Keller, F., Kinzl, H.P., Hunger, H., Knofler, W., Rubling, U. and Merten, W. (1990). The question of the transmissibility of the results of subcutaneous tests of biomaterials from animals to humans. Z. Exp. Chir. Transplant Kunstliche Organe, 23, 23-25
7 Ye, Q., Ohsaki, K., Li, K., Li, D.-J., Zhu, C.-S., Ogawa, T., Tenshin, S. and Takano-Yamamoto, T. (2001). Histological reaction to hydroxyapatite in the middle ear of rats. Auris Nasus Larynx, 28, 131-136   DOI   ScienceOn
8 Lee, B.-H., Kim, Y.D. and Lee, K.H. (2003). XPS study of bioactive graded layer in Ti-In-Nb-Ta alloy prepared by alkali and heat treatments. Biomaterials, 24, 2257-2266   DOI   ScienceOn
9 Matsuno, H., Yokoyama, A., Watari, F., Uo, M. and Kawasaki, T. (2001). Biocompatibility and osteogenesis of refractory metal implants, titanium, hafnium, niobium, tantalum and rhenium. Biomaterials, 22, 1253-1262   DOI   ScienceOn
10 Naganawa, T., Ishihara, Y., Iwata, T., Koide, M., Ohguchi, M., Ohguchi, Y., Murase, Y., Kamei, H., Sato, N., Mizuno, M. and Noguchi, T. (2004). In vitro biocompatibility of a new titanium-29niobium-13tantalum-4.6zirconium alloy with osteoblast-like MG63 cells. J. Periodontol., 75, 1701-1707   DOI   ScienceOn
11 McKay, G.C., Macnair, R., MacDonald, C. and Grant, M.H. (1996). Interactions of orthopaedic metals with an immortalized rat osteoblast cell line. Biomaterials, 17, 1339- 1344   DOI
12 Butler, K.R., Benghuzzi, H.A. and Puckett, A. (2001). Morphometric evaluation tissue-implant reaction associated with ALCAP and TCP bioceramics in vivo. J. Invest. Surg., 14, 139-152   DOI
13 Niinomi, M. (2003). Fatigue performance and cyto-toxicity of low rigidity titanium alloy, Ti-29Nb-13Ta-4.6Zr. Biomaterials, 24, 2673-2683   DOI   ScienceOn
14 Thompson, G.J. and Puleo, D.A. (1996). Ti-6Al-4V ion solution inhibition of osteogenic cell phenotype as a function of differentiation time course in vitro. Biomaterials, 17, 1949-1954   DOI   ScienceOn
15 Udipi, K., Ornberg, R.L., Thurmond II, K.B., Settle, S.L., Forster, D. and Riley, D. (2000). Modification of inflammotry response to implanted biomedical materials in vivo by surface bound superoxide dismutase mimics. J. Biomed. Mater. Res., 51, 549-560   DOI   ScienceOn
16 Murch, A.R., Grounds, M.D., Marshall, C.A. and Papadimitriou, J.M. (1982). Direct evidence that inflammatory multinucleate giant cells form by fusion. J. Pathol., 137, 177- 180   DOI
17 Shimojo, N., Kondo, C., Yamashita, K., Hoshino, T. and Hayakawa, T. (2007). Cytotoxicity analysis of a novel titanium alloy in vitro: adhesion, spreading, and proliferation of human gingival fibroblasts. Biomed. Mater. Eng., 17, 127- 135
18 Walboomers, X.F., Croes, H.J.E., Ginsel, L.A. and Jansen, J.A. (1998). Microgrooved subcutaneous implants in the goat. J. Biomed. Mater. Res., 42, 634-641   DOI   ScienceOn
19 Ito, A., Okazaki, Y., Tateishi, T. and Ito, Y. (1995). In vitro biocompatibility, mechanical properties, and corrosion resistance of Ti-Zr-Nb-Ta-Pd and Ti-Sn-Nb-Ta-Pd alloys. J. Biomed. Mater. Res., 29, 893-899   DOI   ScienceOn
20 Rodriguez, R., Kim, K. and Ong, J.L. (2003). In vitro osteoblast response to anodized titanium and anodized titanium followed by hydrothermal treatment. J. Biomed. Mater. Res., 65A, 352-358   DOI   ScienceOn
21 Jacob, J.T., Burgoyne, C.F., McKinnon, S.J., Tanji, T.M., LaFleur, P.K. and Duzman, E. (1998). Biocompatibility response to modified Baerveldt glaucoma drains. J. Biomed. Mater. Res., 43, 99-107   DOI   ScienceOn
22 Kao, W.J., Zhao, Q.H., Hiltner, A. and Anderson, J.M. (1994). Theoretical analysis of in vivo macrophage adhesion and foreign body giant cell formation on polydimethylsiloxane low density polyethylene and polyetherurethanes. J. Biomed. Mater. Res., 28, 73-79   DOI   ScienceOn
23 Mohammadi, S., Esposito, M., Cucu, M., Ericson, L.E. and Thomsen, P. (2001). Tissue response to hafnium. J. Mater. Sci.: Mater. Med., 12, 603-611   DOI   ScienceOn
24 Okazaki, Y., Gotoh, E., Manabe, T. and Kobayashi, K. (2004). Comparison of metal concentrations in rat tibia tissues with various metallic implants. Biomaterials, 25, 5913- 5920   DOI   ScienceOn
25 Batniji, R.K., Hutchison, J.L., Dahiya, R., Lam, S.L. and Williams, E.F. 3rd. (2002). Tissue response to expanded polytetrafluoroethylene and silicone implants in a rabbit model. Arch. Facial Plast. Surg., 4, 111-113   DOI
26 Kim, M.-J., Kim, C.-W., Lim, Y.-J. and Heo, S.-J. (2006). Microrough titanium surface affects biologic response in MG63 osteoblast-like cells. J. Biomed. Mater. Res., 79A, 1023-1032   DOI   ScienceOn
27 Postiglione, L., Di Domenico, G., Ramaglia, L., Montagnani, S., Salzano, S., Di Meglio, F., Sbordone, L., Vitale, M. and Rossi, G. (2003). Behavior of SaOS-2 cells cultured on different titanium surfaces. J. Dent. Res., 82, 692-696   DOI   ScienceOn
28 Lee, Y.M., Lee, E.J., Yeom, D.S., Kim, D.S., Yee, S.T., Kim, B.I. and Cho, H.W. (2006). Relative biocompatibility evaluation of anodized titanium specimens in vivo and in vitro. J. Life Sci., 16, 302-309   과학기술학회마을   DOI   ScienceOn
29 Li, D.J., Ohsaki, K., Cui, P.C., Ye, Q., Baba, K., Wang, Q.C., Tenshin, S. and Takano-Yamamoto, T. (1999). Thickness of fibrous capsule after implantation of hydroxyapatite in subcutaneous tissue in rats. J. Biomed. Mater. Res., 45, 322-326   DOI   ScienceOn
30 Lehle, K., Lohn, S., Reinerth, G., Schubert, T., Preuner, J.G. and Birnbaum, D.E. (2004). Cytological evaluation of the tissue-implant reaction associated with subcutaneous implantation of polymers coated with titaniumcarboxonitride in vivo. Biomaterials, 25, 5457-5466   DOI   ScienceOn
31 McInnes, A. and Rennick, D.M. (1988). Interleukin 4 induces cultured monocytes/macrophages to form giant multinucleated cells. J. Exp. Med., 167, 598-611   DOI
32 Okazaki, Y. and Gotoh, E. (2005). Comparison of metal release from various metallic biomaterials in vitro. Biomaterials, 26, 11-21   DOI   ScienceOn