Browse > Article

Neurotropism and Expression Pattern of lacZ Inserted PRV-Bartha in Geniculohypothalamic Tract Tracing  

Kim, Jin-Sang (Department of Physical Therapy, Daegu University)
Park, Eun-Se (Department of Physical Therapy, Daegu University)
Cheon, Song-Hee (Department of Physical Therapy, Daegu University)
Kim, Min-Hee (Department of Physical Therapy, Daegu University)
Bang, Hyun-Soo (Department of Physical Therapy, Daegu University)
Kwon, Young-Shil (Department of Physical Therapy, Daegu University)
Lee, Bong-Hee (Department of Anatomy, Cheju National University)
Kim, Young-Chul (Department of Public Health, Keimyung University)
Publication Information
Toxicological Research / v.22, no.4, 2006 , pp. 403-409 More about this Journal
Abstract
To localize the connection between intergeniculate nucleus and suprachisasmatic nucleus through geniculohypothalamic tract in postnatal mongolian gerbil, we injected lacZ inserted PRV-Bartha strain into suprachiasrnatic nucleus and tried to immunostain against it with Rb134 and mouse $anti-{\beta}-galactosidase$. The numbers of immunoreactive neurons in intergeniculate leaflet were $8{\pm}3.2$ in P1 Period, $10{\pm}4.1$ in P3 Period and $13{\pm}6.2$ in P7 Period, and was statistically significant (p<0.05) and had tendency to increase with time consuming. The results showed that intergeniculate leaflet had projected some axons into suprachiasrnatic nucleus through geniculohyptothalamic tract in postnatal mongolian gerbil. But we could not exclude the possibility of direct projections from dorsal and ventral geniculate nuclei into suprachisamatic nucleus completely.
Keywords
Intergeniculate leaflet; Suprachiasmatic nucleus; geniculohypothalamic tract; lacZ; PRV-Bartha; Immuostain;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Arvidsson, U., Riedl, M., Chakrabarti, S., Vulchanova, L., Lee, J.H., Nakano, A.H., Lin, X., Loh, H.H., Law, P.Y., Wessendorf, M.W. and Elde, R. (1995): The -opioid receptor is primarily postsynaptic: combined immunohistochemical localization of the receptor and endogenous opioids. Proc. Natl. Acad. Sci. USA, 92, 5062-5066
2 Babic, N., Mettenleiter, T.C., Flamand, A. and Ugolini, G. (1993): Role of essential glycoprotein gII and gp50 in transneuronal transfer of pseudorabies virus from hypoglossal nerve of mice. J. Virol., 67, 4421-4426
3 Biello, S.M., Janik, D. and Mrosovsky, N. (1994): Neuropeptide Y and behaviorally induced phase shifts. Neuroscience, 62, 273-279   DOI   ScienceOn
4 Bilsky, E.J., Calderon, S.N., Wang, T., Bernstein, R.N., Davis, P., Hruby, V.J., McNutt, R.W., Rothman, R.B., Rice, K.C. and Porreca, F. (1995): SNC 80, a selective, nonpeptidic and systemically active opioid delta agonist. J. Pharm. Exp. Therap., 273, 359-366
5 Byku, M. and Gannon, R.L. (2000): Opioid induced nonphotic phase shifts of hamster circadian activity rhythms. Brain Res., 873, 189-196   DOI   ScienceOn
6 Enquist, L.W., Dubin, J., Whealy, M.E. and Card, J.P. (1994): Complementation analysis of pseudorabies virus gE and gI mutants in retinal ganglion cell neurotropism. J. Virol., 68, 5275-5279
7 Kim, J.S., Enquist, L.W. and Card, J.P. (1999): Circuit-Specific coinfection of neurons in the rat central nervous system with two pseudorabies virus recombinants. J. Virology, 73(11), 9521-9531
8 Maywood, E.S., Okamura, H. and Hastings, M.H. (2002): Opposing actions of neuropeptide Y and light on the expression of circadian clock genes in the mouse suprachiasmatic nuclei. Eur. J. Neurosci., 15, 216-220   DOI   ScienceOn
9 Mead, S., Ebling, F.J., Maywood, E.S., Humby, T., Herbert, J. and Hastings, M.H. (1992): A nonphotic stimulus causes instantaneous phase advances of the light-entrainable circadian oscillator of the Syrian hamster but does not induce the expression of c-fos in the suprachiasmatic nuclei. J. Neurosci., 12, 2516-2522   DOI
10 Wickland, C. and Turek, F.W. (1994): Lesions of the thalamic intergeniculate leaflet block activity-induced phase shifts in the circadian activity rhythm of the golden hamster. Brain Res., 660, 293-300   DOI   ScienceOn
11 Janik, D. and Mrosovsky, N. (1994): Intergeniculate leaflet lesions and behaviorally-induced shifts of circadian rhythms. Brain Res., 651, 174-182   DOI   ScienceOn
12 Meyer-Bernstein, E.L. and Morin, L.P. (1996): Differential serotonergic innervation of the suprachiasmatic nucleus and the intergeniculate leaflet and its role in circadian rhythm modulation. J. Neurosci., 16, 2097-2111   DOI
13 Hastings, M.H., Duffield, G.E., Smith, E.J., Maywood, E.S. and Ebling, F.J. (1998): Entrainment of the circadian system of mammals by nonphotic cues. Chronobiol. Int., 15, 425-445   DOI
14 Card, J.P., Dubin, J.R., Whealy, M.E. and Enquist, L.W. (1995): Influence of infectious dose upon productive replication and transynaptic passage of pseudorabies virus in rat central nervous system. J. Neurovirol., 1, 349-358   DOI
15 Ebling, F.J. (1996): The role of glutamate in the photic regulation of the suprachiasmatic nucleus. Prog. Neurobiol., 50, 109-132   DOI   ScienceOn
16 Morin, L.P. and Blanchard, J.H. (2001): Modulator content of hamster intergeniculate leaflet neurons and their projection to the suprachiasmatic nucleus or visual midbrain. J. Comp. Neurol., 437, 79-90   DOI   ScienceOn
17 Morin, L.P. and Blanchard, J.H. (2001): Neuromodulator content of hamster intergeniculate leaflet neurons and their projection to the suprachiasmatic nucleus or visual midbrain. J. Comp. Neurol., 437, 79-90   DOI   ScienceOn
18 Horikawa, K., Yokota, S., Fuji, K., Akiyama, M., Moriya, T., Okamura, H. and Shibata, S. (2000): Nonphotic entrainment by 5-HT1A/7 receptor agonists accompanied by reduced per1 and per2 mRNA levels in the suprachiasmatic nuclei. J. Neurosci., 20, 5867-5873   DOI
19 Challet, E., Scarbrough, K., Penev, P.D. and Turek, F.W. (1998): Roles of suprachiasmatic nuclei and intergeniculate leaflets in mediating the phase-shifting effects of a serotonergic agonist and their photic modulation during subjective day. J. Biol. Rhythms, 13, 410-421   DOI   ScienceOn
20 Hall, A.C., Earle-Cruickshanks, G. and Harrington, M.E. (1999): Role of membrane conductances and protein synthesis in subjective day phase advances of the hamster circadian clock by neuropeptide Y. Eur. J. Neurosci., 11, 1-9   DOI   ScienceOn
21 Mrosovsky, N. (1991): Double-pulse experiments with nonphotic and photic phase-shifting stimuli. J. Biol. Rhythms., 6, 167-179   DOI   ScienceOn
22 Yannielli, P.C. and Harrington, M.E. (2000): Neuropeptide Y applied in vitro can block the phase shifts induced by light in vivo. NeuroReport, 11, 1587-1591   DOI   ScienceOn
23 McKinley, B., Yannielli, P.C. and Harrington, M.E. (2002): Neuropeptide Y differencially suppresses per1 and per2 mRNA induced by light in the suprachiasmatic nuclei of the golden hamster. J. Biol. Rhythms., 17, 28-39   DOI
24 Morin, L.P. (1999): Serotonin and the regulation of mammalian circadian rhythmicity. Ann. Med., 31, 12-33   DOI   ScienceOn
25 Meyer-Bernstein, E.L. and Morin, L.P. (1998): Destruction of serotonergic neurons in the median raphe nucleus blocks circadian rhythm phase shifts to triazolam but not to novel wheel access. J. Biol. Rhythms., 13, 494-505   DOI   ScienceOn
26 Maywood, E.S. and Mrosovsky, N. (2001): A molecular explanation of interactions between photic and non photic circadian clock resetting stimuli. Gene Expr. Patterns, 1, 27-31   DOI   ScienceOn
27 Edelstein, K. and Amir, S. (1999): The role of the intergeniculate leaflet in entrainment of circadian rhythms to a skeleton photoperiod. J. Neurosci., 19, 372-380   DOI
28 Moga, M.M. and Moore, R.Y. (1997): Organization of neural inputs to the suprachiasmatic nucleus in the rat. J. Comp. Neurol., 389. 508-534   DOI   ScienceOn
29 Biello, S.M. (1995): Enhanced photic phase shifting after treatment with antiserum to neuropeptide. Y. Brain Res., 673, 25-29   DOI   ScienceOn
30 Bobrzynska, K.J. and Mrosovsky, N. (1998): Phase shifting by novelty-induced running: activity dose-response curves at different circadian times. J. Comp. Physiol. A., 182, 251-258   DOI
31 Standish, A., Enquist, L.W., Escardo, J.A. and Schwaber, J.S. (1995): Dendritic morphology of cardiac related medullay neurons defined by circuit specific infection by a recombinant pseudoravies virus expressing $\beta$-galactosidase. J. Neurovirol., 1, 359-368   DOI
32 Morin, L.P. and Blanchard, J. (1995): Organization of the hamster intergeniculate leaflet: NPY and ENK projections to the suprachiasmatic nucleus, intergeniculate leaflet and posterior limitans nucleus. Vis. Neurosci., 12, 57-67   DOI   ScienceOn
33 Huhman, K.L. and Albers, H.E. (1994): Neuropeptide Y microinjected into the suprachiasmatic region phase shifts circadian rhythms in constant darkness. Peptides, 15, 1475-1478   DOI   ScienceOn
34 Akiyama, M., Kouzu, Y., Takahashi, S., Wakamatsu, H., Moriya, T., Maetani, M., Watanabe, S., Tei, H., Sakaki, Y. and Shibata, S. (1999): Inhibition of light- or glutamateinduced mper1 expression represses the phase shifts into the mouse circadian locomotor and suprachiasmatic firing rhythms. J. Neurosci., 19, 1115-1121   DOI
35 Pickard, G.E. (1982): The afferent connections of the suprachiasmatic nucleus of the golden hamster with emphasis on the retinohypothalamic projection. J. Comp. Neurol., 211, 65-83   DOI   ScienceOn
36 Fukuhara, J., McKinley, B., Dirden, J.C., Bittman, E., Tosini, G. and Harrington, M.E. (2001): Neuropeptide Y rapidly reduces Period1 and Period2 mRNA levels in the hamster suprachiasmatic nucleus. Neurosci. Lett., 314, 119-122   DOI   ScienceOn
37 George, S.R., Zastawny, R.L., Briones-Urbina, R., Cheng, R., Nguyen, T., Heiber, M., Kouvelas, A., Chan, A.S. and O'Dowd, B.F. (1994): Distinct distribution of mu, delta and kappa opioid receptor mRNA in rat brain. Biochem. Biophys. Res. Commun, 205, 1438-1444   DOI   ScienceOn