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Inhibition of Cell Proliferation and Induction of Apoptosis by Diallyl Disulfide in Human Colon Cancer Cell lines  

Kim Tae Myoung (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk national University)
Ryu Jae Myun (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk national University)
Kwon Hyun Jung (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk national University)
Woo Koan Sik (Department of Food Science and Technology, College of Agriculture, Chungbuk National University)
Jeong Heon Sang (Department of Food Science and Technology, College of Agriculture, Chungbuk National University)
Hong Jin Tae (College of Pharmacy, Chungbuk National University)
Kim Dae Joong (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk national University)
Publication Information
Toxicological Research / v.21, no.4, 2005 , pp. 355-360 More about this Journal
Abstract
Epidemiological and laboratory studies provide insight into the anti-carcinogenic potential of garlic and its constituent compounds. Garlic is appealing as an anti-carcinogenic agent due to its ability to induce apoptosis in vitro. Diallyl disulfide (DADS) is one of the major components of garlic that used to determine inhibition of cell proliferation and induced apoptosis in human colon cell lines. In this study, human colorectal cancer cell lines (LOVO, HCT-116, SW-480) were exposed to DADS. The inhibitory effects of DADS dose level more than $50\;{\mu}M$ in the cell viability of all cell lines. Cell growth activity inhibits of human colon cancer cell lines. The inhibitory effects of DADS dose level more than $25\~50\;{\mu}M$ in the cell growth using MTT assay. We found that DADS may have the apoptosis action (chromatin condensation, DNA fragmentation) using DAPI staining and increased the expression of caspase-3 at the dose level more than $100\;{\mu}M$, decreased the expression level of $\beta-catenin$ at dose dependent in the western blotting. We suggest that DADS may have a potential candidate as cancer chemopreventive agents.
Keywords
Cancer chemopreventive agent; Garlic; Diallyl disulfide (DADS); Human colorectal cancer cell lines (LOVO, HCT-116, SW480); Apoptosis;
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