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Thirteen-Week Repeated Oral Toxicity Study of Paecilomyces sinclairii in Sprague-Dawely Rats  

Ahn Mi Young (농촌진흥청 농업과학기술원)
Jee Sang Duk (농촌진흥청 농업과학기술원)
Kim Ji Young (농촌진흥청 농업과학기술원)
Han Jea Woong (농촌진흥청 농업과학기술원)
Lee Yang Ki (농촌진흥청 농업과학기술원)
Lee Yang Woo (농촌진흥청 농업과학기술원)
Ryu Kang Sun (농촌진흥청 농업과학기술원)
Lee Byung Mu (성균관대학교 약학대학)
Jung Na Jin (성균관대학교 약학대학)
Kim Sung Nam (녹십자임상검사센타)
Publication Information
Toxicological Research / v.20, no.4, 2004 , pp. 339-348 More about this Journal
Abstract
Paecilomyces sinclairii was administered ad libitum feeding at percentage levels of 0, 1.25, 2.5, 5 and 10 percentage (calculated about 8 g/kg)/feeder for a period of 3 months. There was no observed clinical signs or deaths related to treatment in all groups tested. Therefore, the approximate lethal dose of P. sinclairii was considered to be higher than 8 g/kg in rats. Mild decreases in body weight gain were observed dose-dependently in P. sinclairii treated groups in dose response manner after 2 weeks. Interestingly, the weight of abdominal adipose tissues surrounding epididymides were greatly reduced by this Dongchunghacho, in parallel with the mild increase in body weight gain. However, the absolute weight change of other organs was not observed. There were not significantly different from the control group in urinalysis, ocular examination, hematological, serum biochemical value and histopathological examination. From these results, it is concluded that the no-observed-adverse-effect level (NOAEL) of P. sinclairii is less than 1.25% (1 g/kg) in rats in the present study.
Keywords
Paecilomyces (Isaria) sinclairii; 13-Week repeated toxicity study;
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1 Kim, W.H. (1998): Acute oral toxicity in P. sincfairii in rats, The report of Toxicology Research Center, KRICT (Test No.: G98119
2 Kiho, T, Hui, J., Yamane, A. and Ukai, S. (1993): Polysaccharides in fungi. XXXII. Hypoglycemic activity and chemical properties of a polysaccharide from the cultural mycelium of Cordyceps sinensis. Biol. Pharm. Bull., 16, 1291-1293
3 Kim, H.S., Kim, KB., Lee, S.K., Kwack, S.J., Ahn, M.Y., Choi, B.C. and Lee, B.M. (1996): Subacute oral toxicity of KDRD-002 in rats. Korean J. Toxicol., 12, 323-330
4 Shim, J.Y, Lee, YS., Lim, S.S., Shin, KH., Hyun. J.E., Kim, S.Y and Lee, E.B. (2000): Pharmacological activities of Paecilomyces japonica, a new type Cordyceps sp. Kor. J. Pharmacogn., 31, 63-167
5 Kuo, YC., Tsai, WJ., Shiao, M.S., Chen, C.F. and Lin, C.Y (1996): Cordyceps sinensis as an immunomodulatory agent. Am. J. Chin. Med., 24, 111-125   DOI   ScienceOn
6 Furuya, T, Hirotani, M. and Matsuzawa, M. (1983): N6-(2hydroxyethyl) adenosine, a biologically active compound from cultured mycelia of Cordyceps and Isaria species. Phytochemistry, 22, 2333-2640
7 Liu, X.Y., Wu, cz. and Li, C.C. (1991): Antioxidation of Paecifomyces sinensis (S. pnov.). Chin. J. Trad. Med., 4, 289-303
8 Shim, S.M., Lee, K.R., 1m, KH., Lee, U.Y Lee, M.W and Lee, TS. (2003): The characteristics of cultural conditions for the mycelial growth and fruiting body formation of Paecifomyces sincleitii. Kor. J. Mycol., 31, 8-13
9 Kiho, T, Yamane, A., Hui, J. and Ukai, S. (1996): Polysaccharides in fungi. XXXVI. Hypoglycemic activity of a polysaccharide (CS-F30) from the cultural mycelium of Cordyceps sinensis and its effect on glucose metabolism in ouse liver. Biol. Pharm. Bull., 19, 294-296
10 Kuo, Y.C., Lin, CY, Tsai, W.J., Wu, C.L., Chen, C.F. and Shiao, M.S. (1994): Growth inhibitors against tumor cells in Cordyceps sinensis other than cordycepin and polysaccharides, Cancer Invest., 12, 611-615
11 Kobayashi, Y and Shimizu, D. (1983): Iconography of vegetable wasps and plant warms. Hoikusha Pub. Co. Ltd., Osaka. p.280
12 Kim, H.S., Kwack, S.J. Chun, SA, Han, H.S., Park, H.S., Ahn, MY, Bae, K.H. and Lee, B.M. (1996): Subacute oral toxicity of DWP-311 in Sprague-Dawley rats. J. Appl. Pharmacol., 6, 328-366
13 Ahn, MY, Ryu, KS., Jee, S.D., Kim, I., Kim, J.W, Kim, Y.S., Kim, H.S., Kim, I.S., Kang, S.C., Koo, H.J., Park, YA, Choi, S.M., Kwack, S.J., Yoo, S.D. and Lee, B.M. (2004): Genotoxicity evaluation of Isaria sincfarii extract. J. Toxicol. Environ. Health, Part A, 67, 2037-2044   DOI   ScienceOn
14 Ahn, MY, Kang, S.C., Jung, N.J., Koo, H.J., Kwack, S.J., Yoo, E.J., Jung, JA, Ko, J.K., Ryu, K.S., Jee, S.D., Lee, Yw. and Lee, 8.M. (2003): Acute oral toxicity of Paecifomyces sinclarii in Beagle Dogs. J. Toxicol. Pub. Health, 19, 241-245