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Immunotoxicity of Organophosphorous Pesticides, Pirimiphos-methyl and Methidathion in Balb/c Mice  

Eam Juno H. (식품의약품안전청, 국립독성연구원 독성연구부)
Chung Seung-Tae (식품의약품안전청, 국립독성연구원 독성연구부)
Park Jae Hyun (식품의약품안전청, 국립독성연구원 독성연구부)
Kil Jung Hyun (식품의약품안전청, 국립독성연구원 독성연구부)
Lee Jong Kwon (식품의약품안전청, 국립독성연구원 독성연구부)
Oh Hye Young (식품의약품안전청, 국립독성연구원 독성연구부)
Kim Hyung Soo (식품의약품안전청, 국립독성연구원 독성연구부)
Publication Information
Toxicological Research / v.20, no.4, 2004 , pp. 329-337 More about this Journal
Abstract
Primiphos-methyl and methidathion as organophosphorus (OP) pesticides were tested for their immunotoxic effects on Balb/c mice. Three dose levels of primiphos-methyl (10, 60, or 120 mg/kg/day) and methidathion (0.5, 2.5 or 5.0 mg/kg/day) were administered orally in the mice for 4 weeks. After, changes in body weight gain, relative weight of spleen and thymus, viable splenic cell numbers, surface marker on immune cell, and proliferation activity were investigated. Results showed that neither Pirimiphos-methyl nor methidathion dosages changed significantly body weight, relative thymus and spleen weight, and thymus and spleen cellularities of the mice, but high dose treatment (120 mg/kg) of pirimiphos-methyl significantly decreased relative spleen weight and spleen cellularity of the mice. No alterations were observed in changes of LPS-proliferation response of splenocytes by exposure to any dose of pirimiphos-methyl and methidathion. However, pirimiphos-methyl dosages reduced ConA-proliferation response of splenocytes and both methidathion and pirimiphos-methyl decreased the ability of antibody production to SRBC. The results indicate that 28 days exposure to the high dose of pirimiphos-methyl suppress the function of splenic T and B cell function, and methidathion reduce the immune responsibility of B cell in mice without the changes in lymphoid organ weight or viability of splenocytes. Pirimiphos-methyl is more immunotoxic than methidathion although this has higher general toxicity than that.
Keywords
Organophosphorous pesticide; Pirimiphos-methyl; Methidathion; Immunotoxicity;
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