Oxygen Toxicity: Behavioral Assessment by Swim Test in Mouse Exposed to Hypoxia and Hyperoxia

This study was aimed to evaluate the effect of oxygen on motor activity and toxicity in male mice. The modified Porsolt forced swim test (FST) was used and the distance and time of movement by mice were analyzed in 15。C water bath for 20 minutes using the automatic Ethovision videotracking system. Analyses were carried out before and after 20 minutes of exposure to 10％-70％ concentration of normobaric oxygen. The effects of inspired oxygen tension on the distance and time of movement showed the similar trends, but changes in distance were more prominent. Both the distance and time of movement increased after exposure to 30％ and 40％ oxygen concentration. The distance and time of movement also increased upon exposure to 50％ and 60％ oxygen. In contrast, increases En movement and time under exposure to 21％ oxygen concentration were suppressed when exposed to over 50％ oxygen concentration. With exposure to 10％ oxygen, there was a significant decrease in the distance of movement and a slight suppression of movement time. During the swim test, 12.5％, 37.5％, and 87.5％ of the mice drowned after exposure to 10％, 60％, and 70％ oxygen concentration, respectively. These results suggest that motor activity can be enhanced by inspired oxygen up to 40％ concentration. When hypoxic and hyperoxic oxygen exposure over 50％, motor activity is reduced and toxicity may be induced.

수영 시험 모델에서 산소 농도에 따른 운동성 변화와 독성 평가