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Toxic Bile Salts-Induced Apoptosis of Hepatocytes in Biliary Obstruction Involves Fas-independent Pathway

Oh, Seon-Hee (College of Pharmacy and Medicinal Resources Research Center, Wonkwang University)
Lee, Byung-Hoon (College of Pharmacy and Medicinal Resources Research Center, Wonkwang University)

Publication Information

Toxicological Research / v.19, no.1, 2003 , pp. 67-72 More about this Journal

Abstract

Cholestatic liver injury results from the accumulation of toxic bile salts within the liver. The aim of the present study is to elucidate the changes in expression and cellular localization of apoptosis related proteins in the liver of bile duct-ligated (BDL) rat. Extrahepatic cholestasis was induced by double ligation of the common bile duct and cut between the ligatures. Animals were sacrificed at day 3 and at week 1, 2, 4, 6, and 8 after BDL. The number of TUNEL positive cells was increased significantly after 3 days of BDL, decreased over 2 weeks and remained constant thereafter. Fas expression was not changed and activation of caspase 8 did not occur. Fas immunoreactivity was exclusively observed in the cytoplasm of hepatocytes, indicating that Fas expressed in rat hepatocytes is a soluble form. Hepatocyte apoptosis was associated with Bax expression, which showed a peak at day 3 and decreased over time gradually. Immnunostaining of Bax was observed in hepatocytes and bile duct epithelial cells (BEC) of control and BDL rats. Bcl-2 was increased over time in BDL rats. These results suggest that apoptosis of hepatocytes in BDL rats is independent of Fas and controlled by Bax expression.

Keywords

Bile duct ligation; Apoptosis; Hepatocyte; Fas; Bax;

Citations & Related Records

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