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Antisense bcl-2 Treatment in Human Lung Cancer Cell Lines  

김선미 (식품의약품안전청 생물의약품평가부 생물공학과)
정자영 (식품의약품안전청 생물의약품평가부 생물공학과)
오호정 (식품의약품안전청 생물의약품평가부 생물공학과)
손여원 (식품의약품안전청 생물의약품평가부 생물공학과)
Publication Information
Toxicological Research / v.18, no.4, 2002 , pp. 411-416 More about this Journal
Abstract
Apoptosis, or programmed cell death, is a genetically regulated pathway that is altered in many cancers. Overexpression of bcl-2 leads to resistance to apoptosis and promotes tumorigenesis. To determine the effect of bcl-2 antisense treatment in human lung cancer cell lines, a 20 mer full phosphorothioate oligonucleotide (ODN) targeted at the coding region of the bcl-2 mRNA was synthesized. Western blot analyses were used to examine bcl-2 protein level in five human non-small cell lung cancer (NSCLC) cell lines (NCI-H226, SK-MES-1 NCI-H358, NCI-H522 and NCI-Hl 299) and four human small cell lung cancer (SCLC) cell lines (NCI-H69, NCI-H4l7, HCC-2108 and SW2). Three out of five NSCLC (NCI-H226, SK-MES-1 and NCI-Hl 299) and all of SCLC cell lines expressed Bcl-2 protein. Treatment of these cell with antisense ODN for 48 hours reduced their viability and Bcl-2 protein level. As a conclusion, bcl-2 antisense treatment appears reduction of the Bcl-2 protein levels and cytotoxic effect including apoptosis in human lung cancer cell lines.
Keywords
Apoptoxis; Antisense bcl-2 ODN; NSCLC; SCLC;
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