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Subcutaneous Toxicity Evaluation of a Combination Vaccine against Hantaan and Puumala Viruses in Rats for 4 Weeks

Lee, Su-Hae (College of Veterinary Medicine, Chungbuk National University)
Jung, Eun-Yong (College of Veterinary Medicine, Chungbuk National University)
No, Kyong-Ok (College of Veterinary Medicine, Chungbuk National University)
Sin, Ji-Soon (College of Veterinary Medicine, Chungbuk National University)
Ahn, Chang-Nam (Research Institute, Green Cross Co. Ltd.)
Kim, Dae-Joong (College of Veterinary Medicine, Chungbuk National University)
Nam, Sang-Yoon (College of Veterinary Medicine, Chungbuk National University)
Kang, Jong-Koo (College of Veterinary Medicine, Chungbuk National University)

Publication Information

Toxicological Research / v.18, no.2, 2002 , pp. 139-147 More about this Journal

Abstract

Hantaan (HTNV) and Puumala (PUUV) viruses cause hemorrhagic fever with renal syndrome in human. In the present study, the repeated dose toxicity of the HTNV-PUUV combination vaccine was evaluated in Sprague-Dawley rats. Animals were injected subcutaneously for 28 days with dosages of 0, 0.017, 0.17 and 1.7 dose/kg body weight per day, respectively. No any significant changes of body weight, food and water consumptions were shown. There were no death and clinical findings during the experimental period. In both male and female rats, there were not significant changes in hematological and serum biochemical analysis, urinalysis, and ophthalmoscopic and histopathological examination. These results indicate that the HTNV-PUUV combination vaccine may have no toxic effects and no observed adverse effect level (NOAEL) may be over 1.7 dose/kg/day at subcutaneous route in rats.

Keywords

Repeated dose toxicity; HTNV-PUUV combination vaccine; Rats;

Citations & Related Records

Reference

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