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Cyclooxygenase-2 promotes cell proliferation, migration and invasion in U2OS human osteosarcoma cells  

Lee, Eun-Jeong (Department of Biochemistry and Molecular Biology Kangwon National University College of Medicine)
Choi, Eun-Mi (Department of Biochemistry and Molecular Biology Kangwon National University College of Medicine)
Kim, So-Ra (Department of Biochemistry and Molecular Biology Kangwon National University College of Medicine)
Park, Jung-Hea (Department of Biochemistry and Molecular Biology Kangwon National University College of Medicine)
Kim, Hyun-Sook (Department of Plant Biotechnology School of Bioscience and Biotechnology Kangwon National University)
Ha, Kwon-Soo (Department of Biochemistry and Molecular Biology Kangwon National University College of Medicine)
Kim, Young-Myeong (Department of Biochemistry and Molecular Biology Kangwon National University College of Medicine)
Kim, Sung-Soo (Department of Pharmacology Kangwon National University College of Medicine)
Choe, Myeon (Department of Plant Biotechnology School of Bioscience and Biotechnology Kangwon National University)
Kim, Jong-Il (Department of Biochemistry and Molecular Biology Seoul National University College of Medicine)
Han, Jeong-A (Department of Biochemistry and Molecular Biology Kangwon National University College of Medicine)
Publication Information
Experimental and Molecular Medicine / v.39, no.4, 2007 , pp. 469-476 More about this Journal
Abstract
Osteosarcoma is the most comon primary bone tu-mor, but the pathogenesis is not well understood. While cyclooxygeanse-2 (COX-2) is known to be close-ly associated with tumor growth and metastasis in sev-eral kinds of human tumors, the function of COX-2 in osteosarcoma is unclear. Therefore, to investigate the function of COX-2 in osteosarcoma, we established stable cell lines overexpressing COX-2 in U2OS human osteosarcoma cells. COX-2 overexpression as well as prostaglandin E2 treatment promoted proliferation of U2OS cells. In addition, COX-2 overexpression enhanced mobility and invasiveness of U2OS cells, which was accompanied by increases of matrix metalloproteinase- 2 and -9 (MMP-2 and -9) activities. Selective COX-2 inhibitors, NS-398 and celecoxib, inhibited cell proliferation and abrogated the enhanced mobility, invasiveness and MMP activities induced by COX-2 overexpression. These results suggest that COX-2 is directly associated with cell proliferation, migrationand invasion in human osteosarcoma cells, and the therapeutic value of COX-2 inhibitors should be evaluated continuously.
Keywords
cell movement; cell proliferation; cyclooxygenase-2; matrix metalloproteinases; osteosarcoma;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 24  (Related Records In Web of Science)
Times Cited By SCOPUS : 21
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