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http://dx.doi.org/10.15188/kjopp.2022.10.36.5.187

Effects of Herbal Medicine Complex on Skin Inflammation and Atopic Dermatitis  

Ji-Hee, Choi (Department of Pathology, College of Korean Medicine, Daejeon University)
In-Hwan, Joo (Department of Pathology, College of Korean Medicine, Daejeon University)
Jong-Min, Park (Department of Pathology, College of Korean Medicine, Daejeon University)
Dong-Hee, Kim (Department of Pathology, College of Korean Medicine, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.36, no.5, 2022 , pp. 187-192 More about this Journal
Abstract
The purpose of this study is to examine the effect of herbal medicine complex (HMC) containing Camellia sinensis L., Duchoesna chrysantha, Houttuynia cordata Thunberg, Poncirus trifoliata Rafinesque on skin inflammation and atopic dermatitis. First, we examined the anti-inflammatory effect of HMC in TNF-α induced human keratinocytes (HaCaT cell). Real-time PCR and western blotting were performed to evaluate the expression of inflammatory cytokines (e.g., iNOS, COX-2, IL-6, IL-8) mRNA and protein. Four-weeks old male NC/Nga mice were treated with 1% 2,4-dinitrochlorobenzene (DNCB) solution and used as an atopic dermatitis mice model. And, HMC (200 mg/kg or 400 mg/kg) was administered directly into the stomach of mice for 4 weeks, and blood or serum analysis, tissue staining were performed after oral gavage. As a result HMC inhibited the mRNA expression of iNOS, COX-2, IL-6, and IL-8, which had been increased by TNF-α in HaCaT cells. In addition, the protein expression was also significantly suppressed in the same way as the mRNA expression results. The in vivo experiment results showed that, HMC administration reduced thickening of the epidermis and infiltration of eosinophil into the skin stratum basale compared to DNCB treatment. In addition, HMC administration significantly reduced the inflammatory cytokines (IL-4, IL-5, IL-6, and IL-13) production and immunocyte (white blood cell, lymphocyte, neutrophil, and eosinophil) count compared to DNCB treatment. Moreover, the serum IgE and histamine level was decreased by HMC administration. These results suggest that HMC can be used as effective herbal medicine extract for skin inflammation and atopic dermatitis. And this study may contribute to the development of the herbal medicine-based drug for the treatment of skin inflammation and atopic dermatitis.
Keywords
Herbal medicine complex; HaCaT; Skin inflammation; NC/Nga; Atopic dermatitis;
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Times Cited By KSCI : 7  (Citation Analysis)
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1 Caesar LK, Cech NB. Synergy and antagonism in natural product extracts: when 1+ 1 does not equal 2. Natural product reports. 2019;36(6):869-88.   DOI
2 Williamson EM. Synergy and other interactions in phytomedicines. Phytomedicine. 2001;8(5):401-9.   DOI
3 Kim TH, Ha SY, Yang J-K. Inhibitory Effects of Camellia sinensis Extract on the Development of Atopic Dermatitis-like Lesions in NC/Nga Mice. Journal of the Korean Wood Science and Technology. 2014;42(5):579-89.   DOI
4 Gramza A, Korczak J. Tea constituents (Camellia sinensis L.) as antioxidants in lipid systems. Trends in Food Science & Technology. 2005;16(8):351-8.   DOI
5 Lee JS, Kim IS, Ryu JS, Kim JH, Kim JS, Kim DH, et al. The Inhibitory Effect of Duchesnea chrysantha Extract on the Development of Atopic Dermatitis-like Lesions by Regulating IgE and Cytokine Production in Nc/Nga Mice. Phytotherapy Research. 2012;26(2):284-90.   DOI
6 Lee D-J, Jeon I-H, Kim H-S, Cho I-Y, Jang S-I. Antioxidative and anti-inflammatory effect of ethanol extract from Duchesnea chrysantha. Journal of Physiology & Pathology in Korean Medicine. 2012;26(1):59-66.
7 Cha K-J, Kashif A, Hong MH, Kim G, Lee J-S, Kim IS. Poncirus Trifoliata (L.) Raf. Extract Inhibits the Development of Atopic Dermatitis-like Lesions in Human Keratinocytes and NC/Nga mice. International Journal of Medical Sciences. 2019;16(8):1116.
8 Zhou HY, Shin EM, Guo LY, Zou LB, Xu GH, Lee S-H, et al. Anti-inflammatory activity of 21 (α, β)-methylmelianodiols, novel compounds from Poncirus trifoliata Rafinesque. European journal of pharmacology. 2007;572(2-3):239-48.   DOI
9 Lee HJ, Seo HS, Kim GJ, Jeon CY, Park JH, Jang BH, et al. Houttuynia cordata Thunb inhibits the production of pro-inflammatory cytokines through inhibition of the NFκB signaling pathway in HMC-1 human mast cells. Molecular medicine reports. 2013;8(3):731-6.   DOI
10 Li J, Zhao F. Anti-inflammatory functions of Houttuynia cordata Thunb. and its compounds: A perspective on its potential role in rheumatoid arthritis. Experimental and Therapeutic Medicine. 2015;10(1):3-6.   DOI
11 Hong SC, Eo H-J, Song H-M, Woo S-H, Kim M-K, Lee J-W, et al. Houttuynia cordata Thunberg exhibits anti-tumorigenic activity in human gastric cancer cells. The Korea Journal of Herbology. 2013;28(6):155-60.   DOI
12 Bellanti JA, Settipane RA. The atopic disorders and atopy ... "strange diseases" now better defined! Allergy and asthma proceedings. 2017;38(4):241-2.   DOI
13 Lee JH, Park YM. Proper use of topical corticosteroids. Journal of the Korean Medical Association. 2018;61(10):632-6.   DOI
14 Parhiz H, Roohbakhsh A, Soltani F, Rezaee R, Iranshahi M. Antioxidant and anti-inflammatory properties of the citrus flavonoids hesperidin and hesperetin: an updated review of their molecular mechanisms and experimental models. Phytotherapy Research. 2015;29(3):323-31.   DOI
15 Lucetti DL, Lucetti EC, Bandeira MAM, Veras HN, Silva AH, Leal LKA, et al. Anti-inflammatory effects and possible mechanism of action of lupeol acetate isolated from Himatanthus drasticus (Mart.) Plumel. Journal of Inflammation. 2010;7(1):1-11.   DOI
16 Liu KY, Hu S, Chan BC, Wat EC, Lau CB, Hon KL, et al. Anti-inflammatory and anti-allergic activities of Pentaherb formula, Moutan Cortex (Danpi) and gallic acid. Molecules. 2013;18(3):2483-500.   DOI
17 Boudreau RT, Hoskin DW, Lin TJ. Phosphatase inhibition potentiates IL-6 production by mast cells in response to FcɛRI-mediated activation: involvement of p38 MAPK. Journal of leukocyte biology. 2004;76(5):1075-81.   DOI
18 Dong F, Tan J, Zheng Y. Chlorogenic acid alleviates allergic inflammatory responses through regulating Th1/Th2 balance in ovalbumin-induced allergic rhinitis mice. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research. 2020;26:e923358-1.
19 Hossen MA, Inoue T, Shinmei Y, Minami K, Fujii Y, Kamei C. Caffeic acid inhibits compound 48/80-induced allergic symptoms in mice. Biological and Pharmaceutical Bulletin. 2006;29(1):64-6.   DOI
20 Choi YS, Bae CH, Song S-Y, Kim Y-D. The effect of Epigallocatechin-3-gallate in allergic airway inflammation. Rhinology. 2014;52(4):406-12.   DOI
21 Salamon P, Shoham N, Gavrieli R, Wolach B, Mekori Y. Human mast cells release Interleukin-8 and induce neutrophil chemotaxis on contact with activated T cells. Allergy. 2005;60(10):1316-9.   DOI
22 Surh Y-J, Chun K-S, Cha H-H, Han SS, Keum Y-S, Park K-K, et al. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-κB activation. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2001;480:243-68.
23 Soufli I, Toumi R, Rafa H, Touil-Boukoffa C. Overview of cytokines and nitric oxide involvement in immuno-pathogenesis of inflammatory bowel diseases. World journal of gastrointestinal pharmacology and therapeutics. 2016;7(3):353.
24 Gandhi J, Khera L, Gaur N, Paul C, Kaul R. Role of modulator of inflammation cyclooxygenase-2 in gammaherpesvirus mediated tumorigenesis. Frontiers in microbiology. 2017;8:538.
25 Homey B, Steinhoff M, Ruzicka T, Leung DY. Cytokines and chemokines orchestrate atopic skin inflammation. Journal of Allergy and Clinical Immunology. 2006;118(1):178-89.   DOI
26 Cho W, Choe J. Prostaglandin E2 stimulates COX-2 expression via mitogen-activated protein kinase p38 but not ERK in human follicular dendritic cell-like cells. BMC immunology. 2020;21(1):1-8.   DOI
27 Bryan SA, O'Connor BJ, Matti S, Leckie MJ, Kanabar V, Khan J, et al. Effects of recombinant human interleukin-12 on eosinophils, airway hyper-responsiveness, and the late asthmatic response. The Lancet. 2000;356(9248):2149-53.   DOI
28 Brandt EB, Sivaprasad U. Th2 cytokines and atopic dermatitis. Journal of clinical & cellular immunology. 2011;2(3).
29 Jun J-H, Kang Y-H. The Effects of Scutellaria baicalensis GEORGI on Allergic contact dermatitis induced by DNCB in Mice. The Journal of Dong Guk Oriental Medicine. 1998;7(1):119-33.
30 HanifIn JM. Diagnostic features of atopic dermatitis. Acta Derm Venereol. 1980;92:44-7.   DOI
31 Elias P. The skin as an organ of protection. Dermatology in general medicine. 2003:107-18.
32 Grize L, Gassner M, Wuthrich B, Bringolf-Isler B, Takken-Sahli K, Sennhauser F, et al. Trends in prevalence of asthma, allergic rhinitis and atopic dermatitis in 5-7-year old Swiss children from 1992 to 2001. Allergy. 2006;61(5):556-62.   DOI
33 Correale CE, Walker C, Murphy L, Craig TJ. Atopic dermatitis: a review of diagnosis and treatment. American family physician. 1999;60(4):1191.
34 Ikoma A. Analysis of the mechanism for the development of allergic skin inflammation and the application for its treatment: mechanisms and management of itch in atopic dermatitis. Journal of pharmacological sciences. 2009;110(3):265-9.   DOI
35 Irvine AD, McLean WI, Leung DY. Filaggrin mutations associated with skin and allergic diseases. New England Journal of Medicine. 2011;365(14):1315-27.   DOI
36 Leung DY. Atopic dermatitis: new insights and opportunities for therapeutic intervention. Journal of Allergy and Clinical Immunology. 2000;105(5):860-76.   DOI
37 Whitcup SM, Robinson MR. Development of a dexamethasone intravitreal implant for the treatment of noninfectious posterior segment uveitis. Annals of the New York Academy of Sciences. 2015;1358(1):1-12.   DOI
38 Jackson S, Gilchrist H, Nesbitt Jr LT. Update on the dermatologic use of systemic glucocorticosteroids. Dermatologic therapy. 2007;20(4):187-205.   DOI
39 Lee Y-B. Translational Study on a Chapter of Taeeum-Disease [太陰病篇] in. Journal of Korean Medical classics. 2010;23(2):33-62.
40 Zhou X, Seto SW, Chang D, Kiat H, Razmovski-Naumovski V, Chan K, et al. Synergistic effects of Chinese herbal medicine: a comprehensive review of methodology and current research. Frontiers in Pharmacology. 2016;7:201.