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http://dx.doi.org/10.15188/kjopp.2021.02.35.2.56

Study of Anti-inflammatory Activity of 7 Herbal Prescription Effective for Sleep Disorders and Atopic Dermatitis  

Choi, Min-Jin (Department of Physiology, College of Korean Medicine, Dongguk University)
Nguyen, Ly Thi Huong (Department of Physiology, College of Korean Medicine, Dongguk University)
Jeong, Jeonghwa (Dongje Medical)
Park, Minyoung (College of Korean Medicine, Dongguk University)
Yoon, Joohee (College of Korean Medicine, Dongguk University)
Lee, Byung-Wook (Dongje Medical)
Yang, In-Jun (Department of Physiology, College of Korean Medicine, Dongguk University)
Shin, Heung-Mook (Department of Physiology, College of Korean Medicine, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.35, no.2, 2021 , pp. 56-63 More about this Journal
Abstract
This study was conducted to find a candidate prescription with anti-inflammatory efficacy of 7 herbal prescriptions known to be effective in atopic dermatitis and sleep disorders in Korean medicine. The anti-inflammatory of the 7 herbal prescriptions extracts were evaluated by ELISA assay and Western blot assay. 7 herbal prescriptions, Seongyutang(SU), Danseontoetang(DST), Sotosajahwan(ST), Jisiljagyagsan(JJ), Seokchangpo(SCP), Wiryeongseon(WLS), Gogojohwan(GGJ) 30 % EtoH extract was used for in vitro experiments. In TNF-α + IFN-γ(TI) stimulated HaCaT cells, all 7 herbal prescriptions reduced TARC and MDC production, and JJ strongly reduced TARC and MDC production at 100 ㎍/ml concentration. SCP strongly reduced MDC production at 10 ㎍/ml and 100 ㎍/ml concentration. In addition, in substance P(SP)/CRH stimulated HMC-1 cells, JJ strongly inhibited VEGF production at both 10 and 100 ㎍/ml concentrations. In LPS/CRH stimulated Raw264.7 cells, all 7 herbal prescriptions significantly inhibited TNF-α. PMA + Ionomycin(PI)/CRH stimulated EL4 cells, SU significantly reduced IL-4 production at both concentrations of 10 and 100 ㎍/ml. WLS significantly reduced IL-17 production at both concentrations of 10 and 100㎍/ml. This suggests that 7 herbal prescriptions have anti-inflammatory effects by reducing inflammatory cytokines in keratinocytes, mast cells and macrophages caused by inflammation. Therefore, it is expected that 7 herbal prescriptions that are effective for sleep disorders and atopic dermatitis can be used as therapeutic materials for sleep disorders and diseases associated with atopic dermatitis.
Keywords
Atopic dermatitis; Sleep disturbance; Herbal prescriptions; Comorbidity; Stress;
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