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http://dx.doi.org/10.15188/kjopp.2021.02.35.1.8

Sleep-promoting and Anti-anxiety Effects of Shihogayonggolmoryo-tang in Mice  

Lim, Junsik (College of Korean Medicine, Semyung University)
Leem, Kanghyun (College of Korean Medicine, Semyung University)
Kim, Taeyeon (College of Korean Medicine, Semyung University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.35, no.1, 2021 , pp. 8-14 More about this Journal
Abstract
Shihogayonggolmoryo-tang (ST) is a Korean medical herb cocktail which has been used to treat anxiety induced insomnia. In this study, we will examine sleep-promoting and anti-anxiety effects of ST, and investigate its mechanism. ICR mice were divided into three groups for the first examination : control group (n=11), ST50 group (50 mg/kg, po, n=11), ST200 group (200 mg/kg, po, n=11). Sleep-promoting effect was confirmed by measuring the sleeping duration time and sleeping onset time after thiopental sodium treatment (50 mg/kg, ip). ICR mice were divided into five groups for the second examination : control group (n=11), ST200 group (200 mg/kg, po, n=11), ST200+Flumazenil group (ST 200 mg/kg, po, flumazenil 0.3 mg/kg, ip, n=11), diazepam group (1 mg/kg, ip, n=11), diazepam+flumazenil group (diazepam 1 mg/kg, ip, Flumazenil 0.3 mg/kg, ip, n=11). Anxiety behavior and sleep-promoting effect was confirmed by open field test and measuring the sleeping duration time and sleeping onset time. Expression levels of c-fos in tuberomammillary nucleus (TMN) and ventrolateral preoptic nucleus (VLPO) were analyzed by immunohistochemistry. ST treated group showed significantly decreased anxiety behavior and enhanced sleeping duration time and sleeping onset time concentration dependently. The expression of c-fos was significantly upregulated in VLPO as sleep-inducing center and TMN as downregulated in arousal center by ST treatment. In addition, all effects of ST were reversed by flumazenil. Our results suggest that ST has sleep-promoting and anti-anxiety effects through regulating arousal center (TMN) and sleep-inducing center (VLPO).
Keywords
Shihogayonggolmoryo-tang; Sleep disorder; Sleep latency; Seep onset; Thiopental sodium;
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1 Lee BY, Lee HJ, Kim JW, Choi HY, Kim YJ, Cho SY, Park SU, Ko CN, Park JM. The Efficacy of Shihogayonggolmoryo-tang in Combating Excessive Sweating in Heart Disease Syndrome, Called 心病 in Korean Medicine. J. Int. Korean Med. 2016;37(4):601-8.   DOI
2 Choi WC, Park DM, Kang WC, Lee SR, Jung IC. Interim Report about The Effect of Sihogayonggolmoryeo-tang on the Anxiety of Hwa-byung. J of Oriental Neuropsychiatry 2012:23(4):133-52.   DOI
3 Lee YH. 5 Cases Report of Climacteric Symptoms with Shihogayonggolmoryo-tang. The journal of oriental obstetrics & gynecology. 2013;26(1):121-34.   DOI
4 Kim KS, Kim WC, Kim KO. A Study about trends of using Shihogayong-golm-oryo-tang granule Shihogayonggolmoryo-tang, Herbal extracts, non-prescription medicine, prescription medicine. The Korean journal of oriental medical prescription. 2013;20(1):159-66.
5 Oh DR, Kim Y, Jo A, Im S, Kim CE, Jung MA, Shin J, Kang H, Choi EJ, Kim J, Choi C. Antidepressant-like and Hypnotic Effects of the Herbal Extract Combination of Stauntonia hexaphylla and Vaccinium bracteatum Fruit in Mice. J Physiol & Pathol Korean Med. 2020;34(2):88-96.   DOI
6 Kim HR, Kwon YK, Choi BK, Jung HJ, Baek DG. Effect of Mixture of Atractylodes macrocephala and Amomum villosum Extracts on Body Weight and Lipid Metabolism in High Fat Diet-Induced Obesity Model. J Physiol & Pathol Korean Med. 2020;34(2):75-80.   DOI
7 Jeong HY, Kwon YJ, Park IJ, Kwon JT, Han BH, Lee SP. Effects of the Sanjoin on the Rat Brain. Korean Journal of Biological Psychiatry. 1997;4(1):54-9.
8 Hua R, Yu S, Liu M and Li H. A PCR-Based Method for RNA Probes and Applications in Neuroscience. Front. Neurosci. 2018;12:266.   DOI
9 Koprdova R, Osacka J, Mach M and Kiss A. Acute Impact of Selected Pyridoindole Derivatives on Fos Expression in Different Structures of the Rat Brain. Cellular and Molecular Neurobiology. 2018;38:171-80.   DOI
10 Shin YI, Kim SH. GuksiShanghanlun. Seoul:Jumin Publisher. 2006:133-6.
11 Lobo IA, Harris RA. GABAA receptors and alcohol. Pharmacol Biochem Behav. 2008;90(1):90-4.   DOI
12 Malenka RC, Nestler EJ, Hyman SE. "Chapter 6: Widely Projecting Systems: Monoamines, Acetylcholine, and Orexin". In Sydor A, Brown RY (eds.). Molecular Neuropharmacology: A Foundation for Clinical Neuroscience. 2nd ed. New York:McGraw-Hill Medical. 2009: 175-6.
13 Yang CK. Pharmacological Treatment of Insomnia. J Korean Society of Biological therapes in Psychiatry. 4, 1998;p. 106.
14 Zisapel N. Drugs for insomnia. Expert Opin Emerg Drugs. 2012;17:299-317.   DOI
15 Kim L, Choi YK, Suh KY. The Knowledge and Practice of Sleep Hygiene in Residents of Seoul. J Korean Neuropsychiatr Assoc. 1997;36:712.
16 Zeitzer JM, Dijk DJ, Kronauer R, Brown E, Czeisler C. Sensitivity of the human circadian pacemaker to nocturnal light: melatonin phase resetting and suppression. J Physiol. 2000;526:695-702.   DOI
17 Greenblatt DJ and Roth T. Zolpidem for insomnia. Expert Opin Pharmacother. 2012;13:879-93.   DOI
18 Besnard S, Masse F, Verdaguer M, Cappelin B, Meurice JC, and Gestreau C. Time- and dose-related effects of three 5-HT receptor ligands on the genioglossus activity in anesthetized and conscious rats. Sleep Breath. 2007;11:275-84.   DOI
19 Morin AK. Strategies for treating chronic insomnia. Am J Manag Care. 2006;12:S230-45.
20 Haefely W. Benzodiazepine interactions with GABA receptors. Neuroscience Letters. 1984;47;201-6.   DOI
21 Zammit G. Comparative tolerability of newer agents for insomnia. Drug Saf 2009;32:735-48.   DOI
22 Moon JJ, An GS, Kim JB, Park WS, Park JH, Kim DH et al. Precise Interpretation of 'Treatise on Cold Damage Diseases'. Yongin:Hanuimunhwasa. 2017:p. 239-40.
23 Kim SO. The Effect of Shiho Ka Yongol MoryoTang on the Contents of Total Cholesterol, Triglyceride and Phospholipid in the Serum of Rabbit. Kyunghee College of Pharmacy collection of dissertations. 1978;6(1):17-22.
24 Kim HY, Lee TH. Effect of Sihogayonggolmoryeotang on SPS-induced PTSD in Rats. Herbal formula science. 2019;27(2):121-36.   DOI
25 Song U, Kim SH. The Effects of Kakamsihokayongkolmoryeotang on the Hyperlipidemia. The Journals of the Korean Oriental Medical Society. 1993;26:332-47.
26 Lee SJ. The operation of Shihogayonggolmoryo-tang on the nervous disease. The Journals of the Korean Oriental Medical Society. 1976;13(1):86-8.
27 Seo SH, Yim HJ, Jung IC, Lee SR. The Clinical study on 1 case of Patient with Tremor by Bok-Jin. Institute of Oriental Medinine Daejeon University collection of dissertations. 2007;16(1):69-79.
28 Lee JH, Lee JS, Chung SH. A Clinical Report about Meralgia paresthtica Treated with Sihogayonggolmoryo-tang. The Journals of Oriental Rehab Med. 2002;12(3):157-67.