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http://dx.doi.org/10.15188/kjopp.2020.12.34.6.326

Changes in Cerebral Blood flow Following Fermented Garlic Extract Solution with High Content of Nitrite  

Yu, Hyeok (Department of Emergency Medicine, Wonkwang University School of Medicine)
Rong, Zhang Xiao (Center for Nitric Oxide Metabolite at Wonkwang University & Department of Physiology, Wonkwang University)
Koo, Ho (Center for Nitric Oxide Metabolite at Wonkwang University & Department of Physiology, Wonkwang University)
Chun, Hyun Soo (Humanenos Co. LTD)
Yoo, Su Jin (Department of Emergency Medicine, Wonkwang University School of Medicine)
Kim, Min Sun (Center for Nitric Oxide Metabolite at Wonkwang University & Department of Physiology, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.34, no.6, 2020 , pp. 326-333 More about this Journal
Abstract
Nitrate-nitrite-nitric oxide (NO) pathway is a major alternative source of NO and is essential for NO - dependent physiological functions in body. Food supplements having nitrate/nitrite can improve metabolic syndromes including hypertension through antioxidant activity or vasodilation. The purpose of this study was to observe the effects of fermented garlic (F. garlic) having high concentration of NO2- on changes in blood flow and nitric oxide synthesis in the cerebral cortex of rodents. The generation of nitric oxide detected by a chemi-luminescence detector was higher in F. Garlic compared with NaNO2 solution under artificial gastric juice with pH 2.0. Ether F. garlic or NaNO2 diluted with artificial cerebrospinal fluid was directly applied into around the needle probe of laser Doppler flow meter that was located on epidural surface of the cortex. Direct application of F. garlic resulted in increase of cerebral blood flow detected by a laser Doppler flow meter with a dose-dependent manner. Compared with NaNO2 solution, F. garlic produced changes in cerebral blood flow at lower concentration of NO2-. Pretreatment of methylene blue, a guanylyl cyclase inhibitor prevented upregulation of cerebral blood flow by the treatment of F. garlic. In addition, the application of F. garlic with 250, 500ppm of NO2- caused significantly the production of NO in the cortical tissue but NaNO2 solution with 500ppm of NO2- did not. In summary, these results suggested that F. garlic with high content of NO2- induce increase in cerebral blood flow through nitric oxide-dependent signal pathway.
Keywords
Nitrite; Nitric oxide; Cerebral blood flow; Garlic; Fermentation;
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