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http://dx.doi.org/10.15188/kjopp.2020.08.34.4.170

Palmijihwang-tang Alleviates Cisplatin-induced Nephrotoxicity through Inhibiting ROS Production and p53 Activation  

Ju, Sung-Min (Department of Pathology, College of Korean Medicine, Wonkwnag University)
Park, Seo-Hee (Department of Preventive Medicine, College of Korean Medicine, Wonkwnag University)
Chong, Myong-Soo (Department of Preventive Medicine, College of Korean Medicine, Wonkwnag University)
Jeon, Byung-Hun (Department of Pathology, College of Korean Medicine, Wonkwnag University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.34, no.4, 2020 , pp. 170-176 More about this Journal
Abstract
Palmijihwang-tang is an herbal formula frequently used to treat many symptoms, such as lumbago, pollakiuria, cold hands and feet, nephritis, sterilitas virilis, and prostatic disorders. The aim of this study was to investigate the effects of Palmijihwang-tang on cisplatin-induced nephrotoxicity in rat kidney proximal tubular NRK-52E cells. NRK-52E cells were treated with Palmijihwang-tang in absence or presence of 30 µM cisplatin for 12 or 24 h. Palmijihwang-tang at concentrations of 50-800 ㎍/ml did not change the cell viability in NRK-52E cells, and showed no significant toxicity. Palmijihwang-tang at concentrations of 400 and 800 ㎍/ml significantly increased the cell viability and reduced apoptotic cells in NRK-52E cells exposed to cisplatin. Also, Palmijihwang-tang markedly inhibited cisplatin-induced caspase-3 activation, PARP cleavage, ROS production and p53 activation in NRK-52E cells. Furthermore, Palmijihwang-tang did not interfere with the antitumor activity of cisplatin in AGS and A549 cancer cells. Particularly, Palmijihwang-tang enhanced antitumor activity of cisplatin in A549 cells. Taken together, these results suggest that Palmijihwang-tang ameliorated cisplatin-induced nephrotoxicity through reduction of ROS production and p53 activation, and did not interrupt antitumor efficacy of cisplatin against cancer cells.
Keywords
A549; AGS; Antitumor activity; Cisplatin; Nephrotoxicity; NRK-52E; Palmijihwang-tang;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Lee MH, Son IC. Effect of Aqua-Acupuncture of Yukmijihwangtang and Palmijihwangtang Water Extracts on the Renal Function. J Acupunct Res. 1998;15(2):255-77.
2 Sakamoto S, Kudo H, Kawasaki T, Kasahara N, Okamoto R. Effect of Ba-Wei-Di-Huang-Wan (Hachimi-Jio-Gan) on Thymidine Kinase and Its Isozyme Activities in the Prostate Glands in Rats. 1988;16(1-2):29-36.   DOI
3 Usuki S. Hachimijiogan Produces Testosterone in Adult Rat Testes. Am J Chin Med. 1988;16(3-4):93-105.   DOI
4 Kim HY, Yokozawa T, Cho EJ, Yamabe N. Protective Effects of the Chinese Prescription Hachimi-jio-gan Against Diabetic Oxidative Stress. J Pharm Pharmacol. 2004;56(10):1299-305.   DOI
5 Furuya Y, Kawakita T, Nomoto K. Immunomodulating Effect of a Traditional Japanese Medicine, Hachimi-Jio-Gan (Ba-Wei-Di-Huang-Wan), on Th1 Predominance in Autoimmune MRL/MP-lpr/lpr Mice. Int Immunopharmacol. 2001;1(3):551-9.   DOI
6 Oka H, Goto H, Koizumi K, Nakamura S, Tsuneyama K, Zhou Y, Jo M, Fujimoto T, Sakurai H, Shibahara N, Saiki I, Shimada Y. Effect of Hachimijiogan Against Renal Dysfunction and Involvement of Hypoxia-Inducible Factor-1α in the Remnant Kidney Model. Evid Based Complement Alternat Med 2011;2011:348686.
7 Jiang M, Wei Q, Pabla N, Dong G, Wang CY, Yang T, Smith SB, Dong Z. Effects of Hydroxyl Radical Scavenging on Cisplatin-Induced p53 Activation, Tubular Cell Apoptosis and Nephrotoxicity. Biochem Pharmacol. 2007;73(9):1499-510.   DOI
8 Ju SM, Pae HO, Kim WS, Kang DG, Lee HS, Jeon BH. Role of Reactive Oxygen Species in p53 Activation During Cisplatin-Induced Apoptosis of Rat Mesangial Cells. Eur Rev Med Pharmacol Sci. 2014;18(8):1135-41.
9 Wang D, Lippard SJ. Cellular Processing of Platinum Anticancer Drugs. Nat Rev Drug Discov. 2005;4(4):307-20.   DOI
10 Cohen SM, Lippard SJ. Cisplatin: From DNA Damage to Cancer Chemotherapy. Prog Nucleic Acid Res Mol Biol. 2001;67:93-130.   DOI
11 Casares C, Ramirez-Camacho R, Trinidad A, Roldan A, Jorge E, Garcia-Berrocal JR. Reactive Oxygen Species in Apoptosis Induced by Cisplatin: Review of Physiopathological Mechanisms in Animal Models. Eur Arch Otorhinolaryngol. 2012;269(12):2455-9.   DOI
12 Pabla N, Dong Z. Cisplatin Nephrotoxicity: Mechanisms and Renoprotective Strategies. Kidney Int. 2008;73(9):994-1007.   DOI
13 Kim TW, Kim YJ, Park SR, Seo CS, Ha H, Shin HK, Jung YJ. Chrysanthemum Indicum Attenuates Cisplatin-Induced Nephrotoxicity Both in Vivo and in Vitro. Nat Prod Commun. 2015;10(3):397-402.
14 Oh RS, Kim YH, Ahn BG. Study on The Effects of Parnijiwhanghan and Yukmijiwhanghan. Korean J Orient Med Pathol. 1996;10(2):12-7.
15 Nakagawa T, Yokozawa T, Yamabe N, Rhyn DY, Goto H, Shimada Y, Shibahara N. Long-term Treatment With Hachimi-jio-gan Attenuates Kidney Damage in Spontaneously Diabetic WBN/Kob Rats. J Pharm Pharmacol. 2005;57(9):1205-12.   DOI
16 Yamabe N, Yokozawa T, Kim HY, Cho EJ. Protective Effect of Hachimi-jio-gan Against Renal Failure in a Subtotal Nephrectomy Rat Model. J Pharm Pharmacol. 2005;57(12):1637-44.   DOI
17 Conklin KA. Dietary Antioxidants During Cancer Chemotherapy: Impact on Chemotherapeutic Effectiveness and Development of Side Effects. Nutr Cancer. 2000;37(1);1-18.   DOI
18 Bhatt K, Zhou L, Mi QS, Huang S, She JX, Dong Z. MicroRNA-34a Is Induced via p53 During Cisplatin Nephrotoxicity and Contributes to Cell Survival. Mol Med. 2010;16(9-10):409-16.   DOI
19 Rybak LP. Mechanisms of Cisplatin Ototoxicity and Progress in Otoprotection. Curr Opin Otolaryngol Head Neck Surg. 2007;15(5):364-9.   DOI
20 Barabas K, Milner R, Lurie D, Adin C. Cisplatin: A Review of Toxicities and Therapeutic Applications. Vet Comp Oncol. 2008;6(1):1-18.   DOI
21 Cubeddu LX. Mechanisms by Which Cancer Chemotherapeutic Drugs Induce Emesis. Semin Oncol. 1992;19(6 Suppl 15):2-13.
22 Hanigan MH, Devarajan P. Cisplatin Nephrotoxicity: Molecular Mechanisms. Cancer Ther. 2003;1:47-61.
23 McWhinney SR, Goldberg RM, McLeod HL. Platinum Neurotoxicity Pharmacogenetics. Mol Cancer Ther. 2009;8: 10-16.   DOI
24 Kintzel PE. Anticancer Drug-Induced Kidney Disorders. Drug Saf. 2001;24(1):19-38.   DOI
25 Han JH, Kim GY. Hanbangyakrihak, Seoul: Eui Seong Dang Publishing Co. p. 2008. p. 169.
26 Kim SK, Kwon DA, Lee HS, Kim HK, Kim WK. Preventive Effect of the Herbal Preparation, HemoHIM, on Cisplatin-Induced Immune Suppression. Evid Based Complement Alternat Med. 2019;2019:3494806.
27 Kelland L. The Resurgence of Platinum-Based Cancer Chemotherapy. Nat Rev Cancer. 2007;7(8):573-84.   DOI
28 Kim HJ, Lee JY, You BR, Soo DE, Kim MR. Antioxidant Activities of Rehmannia glutinosa by Traditional Methods. Korean J Medicinal Crop Sci. 2011;19(5):341-6.   DOI
29 Ju SM, Park JM, Jeon BJ, Yang HM, Hong JE, Kim IG, Kim WS, Jeon BH. Preventive Effect of Puerariae Radix and Rehmanniae Radix Preparata on Cisplatin-induced Rat Mesangial Cell Apoptosis. J Physiol & Pathol Korean Med. 2008;22(5):1140-6.
30 Seo SJ, Kim YH, Park RG, So HS, Jeon BH, Shin MK, Jung SY, Kim KY, You YO. Protective Effect of Rehmannia Radix Preparata Extract on the Cisplatin-induced Cytotoxicity of HEI-OC1 Cells via Scavenging of Free Radicals. J Physiol & Pathol Korean Med. 2005;19(5):1349-55.
31 Jun SA, Lee H. The Effects of Dokhwaljihwang-tang Intravenous Pharmacopuncture on Cisplatin-Induced Emesis and Gastrointestinal Mobility Disorder in Rats. J Acupunct Res. 2017;34(3):39-48.   DOI
32 Ahn BS, Kim SK, Kim HN, Lee JH, Lee JH, Hwang DS, Bae H, Min BI, Kim SK. Gyejigachulbu-Tang Relieves Oxaliplatin-Induced Neuropathic Cold and Mechanical Hypersensitivity in Rats via the Suppression of Spinal Glial Activation. Evid Based Complement Alternat Med. 2014;2014:436842.
33 Park YC, KIM JB, Kook YB, Lee SD. Pharmacological and Toxicological review of Yukmijihwang-tang (Hwan). Herb Formula Sci. 2012;20(1):13-24.   DOI
34 Zhang ZJ. Gumgueyolak. Beijing: Inminwisaeng publisher; 1989. p. 157.