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http://dx.doi.org/10.15188/kjopp.2020.04.34.2.67

Study on Skin Whitening and Antioxidant Effect of Anemarrhenae Rhizoma Extract  

Choi, Chanhun (College of Korean Medicine, Dongshin University)
Jeong, Hyun Woo (College of Korean Medicine, Dongshin University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.34, no.2, 2020 , pp. 67-74 More about this Journal
Abstract
The objective of this study is to investigate the skin whitening and antioxidant effects of the Anemarrhenae Rhizoma extract (ARE). Following the previously studied method, we examined the inhibitory effects of melanin synthesis and tyrosinase activity by using B16F10 cells. First, we measured the Diphenylpicrylhydrazyl (DPPH) assay, nitrite scavenging activity, and superoxide dismutase-like activity to verifying antioxidant efficacy according to skin whitening. In addition, we confirmed the skin whitening efficacy of ARE by measuring gene expression associated with a skin whitening by the Reverse transcription polymerase chain reaction (RT-PCR) method in B16F10 cells. In this study, we confirmed that ARE has skin whitening and antioxidant effects at high concentrations. In particular, ARE at a concentration of 500 ㎍/ml inhibited the expression of Tyrosinase, TRP-2 (tyrosinase-related protein), and MITF (microphthalmia transcription factor) genes better than Arbutin. In conclusion, our results confirmed that ARE has the potential for development as a skin whitening efficacy substance.
Keywords
Anemarrhenae Rhizoma; Melanin; Tyrosinase; Skin whitening; Antioxidant;
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Times Cited By KSCI : 5  (Citation Analysis)
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