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http://dx.doi.org/10.15188/kjopp.2019.12.33.6.356

Effects of Fermented Scutellaria Baicalensis Extract on H2O2 - Induced Impairment of Long-term Potentiation in Hippocampal CA1 Area of Rats  

Heo, Jun Ho (Department of Emergency Medicine, Wonkwang University School of Medicine)
Rong, Zhang Xiao (Department of Physiology, Wonkwang University School of Medicine & Brain Science Institute at Wonkwang University)
Kim, Min Sun (Department of Physiology, Wonkwang University School of Medicine & Brain Science Institute at Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.33, no.6, 2019 , pp. 356-362 More about this Journal
Abstract
Scutellaria baicalensis (SB) has widely used in the treatment for various brain diseases in the field of Oriental medicine. Biofermantation of SB can make major chemical constituents of SB to pass blood-brain barrier easily and to have more potent anti-oxidant ability. There is a little information about the contribution of fermented SB (FSB) to the formation or maintenance of the neural plasticity in the hippocampus. The purpose of this study was to evaluate effects of FSB extract on hydrogen peroxide (H2O2) - induced impairments of the induction and maintenance of long-term potentiation (LTP), an electrophysiological marker for the neural plasticity in the hippocampus. From hippocampal slices of rats, the field excitatory postsynaptic potentials (fEPSPs) were evoked by the electrical stimulation to the Schaffer collaterals - commissural fibers in the CA1 areas and LTP by theta-burst stimulation by using 64 - channels in vitro multi-extracellular recording system. In order to induce oxidative stress to hippocampal slices two different concentrations (200, 400 μM) of H2O2 were given to the perfused aCSF before and after the LTP induction, respectively. The ethanol extract of FBS with concentration of 25 ㎍/ml, 50 ㎍/ml was diluted in perfused aCSF that had 200 μM H2O2, respectively. Oxidative stress by the treatment of H2O2 resulted in decrease of the induction rate of LTP in the CA1 area with a dose - dependent manner. However, the ethanol extract of FSB prevented the reduction of the induction rate of LTP caused by H2O2 - induced oxidative stress with a dose - dependent manner. These results may support a potential application of FSB to ameliorate impairments of hippocampal dependent neural plasticity or memory caused by oxidative stress.
Keywords
Scutellaria baicalensis; Fermentation; Oxidative stress; Hippocampus; Long - term potentiation;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Wang W, Wang F, Yang YJ, Hu ZL, Long LH, Fu H, et al., The flavonoid baicalein promotes NMDA receptor-dependent long-term potentiation and enhances memory, British journal of pharmacology. 2011;162:1364-79.   DOI
2 Lee GW, Kim JH, Kim MS, Reduction of long-term potentiation at Schaffer collateral-CA1 synapses in the rat hippocampus at the acute stage of vestibular compensation, The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology. 2017;21:423-8.   DOI
3 Impey S, Mark M, Villacres EC, Poser S, Chavkin C, Storm DR, Induction of CRE-mediated gene expression by stimuli that generate long-lasting LTP in area CA1 of the hippocampus, Neuron. 1996;16:973-82.   DOI
4 Bekinschtein P, Cammarota M, Izquierdo I, Medina JH, BDNF and memory formation and storage, The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry. 2008;14:147-56.   DOI
5 Chen SD, Wu CL, Hwang WC, Yang DI, More Insight into BDNF against Neurodegeneration: Anti-Apoptosis, Anti-Oxidation, and Suppression of Autophagy, International journal of molecular sciences. 2017;18.
6 Kamsler A, Segal M, Hydrogen peroxide as a diffusible signal molecule in synaptic plasticity, Molecular neurobiology. 2004;29:167-78.   DOI
7 Serrano F, Klann E, Reactive oxygen species and synaptic plasticity in the aging hippocampus, Ageing research reviews. 2004;3:431-43.   DOI
8 Klann E, Thiels E, Modulation of protein kinases and protein phosphatases by reactive oxygen species: implications for hippocampal synaptic plasticity, Progress in neuro-psychopharmacology & biological psychiatry. 1999;23:359-76.   DOI
9 Fong SYK, Li C, Ho YC, Li R, Wang Q, Wong YC, Brain Uptake of Bioactive Flavones in Scutellariae Radix and Its Relationship to Anxiolytic Effect in Mice, 2017;14:2908-16.   DOI
10 Hamada H, Hiramatsu M, Edamatsu R, Mori A, Free radical scavenging action of baicalein, Archives of biochemistry and biophysics. 1993;306:261-6.   DOI
11 Jeon SJ, Rhee SY, Seo JE, Bak HR, Lee SH, Ryu JH, et al., Oroxylin A increases BDNF production by activation of MAPK-CREB pathway in rat primary cortical neuronal culture, Neuroscience research. 2011;69:214-22.   DOI
12 Xiong Z, Jiang B, Wu PF, Tian J, Shi LL, Gu J, et al., Antidepressant effects of a plant-derived flavonoid baicalein involving extracellular signal-regulated kinases cascade, Biological & pharmaceutical bulletin. 2011;34:253-9.   DOI
13 Kim DH, Jeon SJ, Son KH, Jung JW, Lee S, Yoon BH, et al., Effect of the flavonoid, oroxylin A, on transient cerebral hypoperfusion-induced memory impairment in mice, Pharmacology, biochemistry, and behavior. 2006;85:658-68.   DOI
14 Kim DH, Jeon SJ, Son KH, Jung JW, Lee S, Yoon BH, et al., The ameliorating effect of oroxylin A on scopolamine-induced memory impairment in mice, Neurobiology of learning and memory. 2007;87:536-46.   DOI
15 Jeon SJ, Bak H, Seo J, Han SM, Lee SH, Han SH, et al., Oroxylin A Induces BDNF Expression on Cortical Neurons through Adenosine A2A Receptor Stimulation: A Possible Role in Neuroprotection, Biomolecules & therapeutics. 2012;20:27-35.   DOI
16 Xie L, Zheng Y, Ma Z, Sun Q, Li X, Wang ZL, et al., A comprehensive review on phytochemistry, pharmacology, and flavonoid biosynthesis of Scutellaria baicalensis, The Journal of pharmacy and pharmacology. 2018;56:465-84.
17 Salim S, Oxidative Stress and the Central Nervous System, The Journal of pharmacology and experimental therapeutics. 2017;360:201-5.   DOI
18 Cobley JN, Fiorello ML, Bailey DM, 13 reasons why the brain is susceptible to oxidative stress, Redox biology. 2018;15:490-503.   DOI
19 Carvalho AN, Firuzi O, Gama MJ, Horssen JV, Saso L, Oxidative Stress and Antioxidants in Neurological Diseases: Is There Still Hope?, Current drug targets. 2017;18:705-18.   DOI
20 Zhao T, Tang H, Scutellaria baicalensis Georgi. (Lamiaceae): a review of its traditional uses, botany, phytochemistry, pharmacology and toxicology, 2019;71:1353-69.   DOI
21 Gaire BP, Moon SK, Kim H, Scutellaria baicalensis in stroke management: nature's blessing in traditional Eastern medicine, Chinese journal of integrative medicine. 2014;20:712-20.   DOI
22 Xu C, Ji GE, Bioconversion of flavones during fermentation in milk containing Scutellaria baicalensis extract by Lactobacillus brevis, Journal of microbiology and biotechnology. 2013;23:1422-7.   DOI
23 Gustafsson B, Wigstrom H, Long-term potentiation in the hippocampal CA1 region: its induction and early temporal development, Progress in brain research. 1990;83:223-32.   DOI
24 Fong SY, Wong YC, Zuo Z, Development of a SPE-LC/MS/MS method for simultaneous quantification of baicalein, wogonin, oroxylin A and their glucuronides baicalin, wogonoside and oroxyloside in rats and its application to brain uptake and plasma pharmacokinetic studies, Journal of pharmaceutical and biomedical analysis. 2014;97:9-23.   DOI
25 Sowndhararajan K, Deepa P, Kim M, Park SJ, Kim S, Baicalein as a potent neuroprotective agent: A review, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2017;95:1021-32.   DOI
26 Kim DH, Lee Y, Lee HE, Park SJ, Jeon SJ, Jeon SJ, et al., Oroxylin A enhances memory consolidation through the brain-derived neurotrophic factor in mice, Brain research bulletin. 2014;108:67-73.   DOI
27 Gu XH, Xu LJ, Liu ZQ, Wei B, Yang YJ, Xu GG, et al., The flavonoid baicalein rescues synaptic plasticity and memory deficits in a mouse model of Alzheimer's disease, Behavioural brain research. 2016;311:309-21.   DOI
28 Kim MS, Yang JH, Kong BM, Manufacture Method of combination of fermented Scutellaria baicalensis with Gastrodia elata for prevention and treatment of central nerous system disease. Korean Intellectual Property Office. 2015;10-2015-0062531.
29 Chen L, Liu B, Relationships between Stress Granules, Oxidative Stress, and Neurodegenerative Diseases, 2017;2017:1809592.
30 Winder DG, Sweatt JD, Roles of serine/threonine phosphatases in hippocampal synaptic plasticity, Nature reviews. Neuroscience. 2001;2:461-74.
31 Bliss TV, Collingridge GL, A synaptic model of memory: long-term potentiation in the hippocampus, Nature. 1993;361:31-9.   DOI
32 Ocampo AC, Squire LR, Clark RE, Hippocampal area CA1 and remote memory in rats, Learning & memory (Cold Spring Harbor, N.Y.). 2017;24:563-8.   DOI
33 Sen A, Hongpaisan J, Hippocampal microvasculature changes in association with oxidative stress in Alzheimer's disease, Free radical biology & medicine. 2018;120:192-203.   DOI
34 Shao ZH, Li CQ, Vanden Hoek TL, Becker LB, Schumacker PT, Wu JA, et al., Extract from Scutellaria baicalensis Georgi attenuates oxidant stress in cardiomyocytes, Journal of molecular and cellular cardiology. 1999;31:1885-95.   DOI
35 Yoon JJ, Jeong JW, Choi EO, Kim MJ, Hwang-Bo H, Kim HJ, et al., Protective effects of Scutellaria baicalensis Georgi against hydrogen peroxide-induced DNA damage and apoptosis in HaCaT human skin keratinocytes, EXCLI journal. 2017;16:426-38.   DOI
36 Li Y, Zhao J, Holscher C, Therapeutic Potential of Baicalein in Alzheimer's Disease and Parkinson's Disease, CNS drugs. 2017;31:639-52.   DOI
37 Chon H, Kim G, Kim S, Comparison of aqueous plant extracts before and after fermentation with Lactobacillus paracasei LS-2 on cytokine induction and antioxidant activity, Natural product communications. 2010;5:1277-82.