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http://dx.doi.org/10.15188/kjopp.2019.10.33.5.267

Study of ShengmaisanJiaweifang Extracts on the Inhibitory Effects of Melanin Synthesis and Superoxide Dismutase Activity  

Jeong, Hyun Woo (College of Korean Medicine, Dongshin University)
Choi, Chan Hen (College of Korean Medicine, Dongshin University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.33, no.5, 2019 , pp. 267-274 More about this Journal
Abstract
This study aims to evaluate the effects of Shengmaisan (SMS) and three types of ShengmaisanJiaweifang on the inhibitory effect of melanin synthesis in B16F10 cells, the mechanism of action through tyrosinase, and the antioxidant effect through superoxide dismutase (SOD) activity. In this study, we used ShengmaisanJiaweifangs (SMS, SMSRR, SMSAD, SMSAR) to research the whitening effects in B16F10 cell lines. Shengmaisan (SMS) was a herbal medicine composed of Ginseng Radix, Liriopis Tuber, and Schisandrae Fructus. ShengmaisanJiaweifangs included SMSRR (SMS added with Rehmanniae Radix), SMSAD (SMS added with Asparagi Radix) and SMSAR (SMS added with Astragali Radix). We measured the cell viability, the inhibition rate of the melanin biosynthesis, and the activity of tyrosinase and SOD in malignant melanoma, B16F10 cells, to survey the whitening effect and the mechanism of the impact on the sample. As a result, SMSRR significantly suppressed the cell viability of B16F10 at more than $500{\mu}g/m{\ell}$ and significantly inhibited the generation of melanin induced by ${\alpha}$-MSH at more than $250{\mu}g/m{\ell}$. SMSRR ($500{\mu}g/m{\ell}$) decreased the activity of tyrosinase while increased the activity of SOD. Therefore, we considered that the SMSRR would be able to produce high value-added products more SMS if used as a commercial.
Keywords
Shengmaisanjiaweifang; Rehmanniae Radix; Melanin synthesis; Tyrosinase; Superoxide dismutase;
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Times Cited By KSCI : 10  (Citation Analysis)
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