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http://dx.doi.org/10.15188/kjopp.2019.06.33.3.169

Effects of Juglans regia Complex Extract on Osteoclast Differentiation from Bone Marrow Derived Macrophage  

Kong, Hae Jin (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Daejeon University)
Kang, Jae Hui (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Daejeon University)
Ryu, Hwa Yeon (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Daejeon University)
Lee, Hyun (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.33, no.3, 2019 , pp. 169-174 More about this Journal
Abstract
The purpose of this study was to evaluate the inhibitory effects of Juglans regia complex extract(JCE) consisted of Juglans regia, Eucommia ulmoides, Eleutherococcus senticosus and Zingiber officinale on osteoclast differentiation. Cell toxicity test by using CCK-8, TRAP activity and TRAP positive multi-nucleated cell counting were performed to evaluate inhibitory effect on differentiation of osteoclast from bone marrow derived macrophages(BMMs) induced by receptor activator of nuclear $factor-{\kappa}B$ ligand(RANKL). As a result, JCE inhibited RANKL-induced osteoclast differentiation in BMMs dose-dependently without cytotoxicity. These results suggest that JCE may have a potential role for treating bone lytic diseases such as osteoporosis.
Keywords
Juglans regia complex extract(JCE); Osteoporosis; Osteoclast; TRAP;
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1 National college of Korean medicine collaborative textbook compilation committee. Herbology. 2nd ed; Seoul: Younglimsa, 2011.
2 Papoutsi Z, Kassi E, Chinou I, Halalbalaki M, Skaltsounis LA, Moutsatsou P. Walnut extract(Juglans regia L.) and its component ellagic acid exhibit anti-inflammatory activity in human aorta endothelial cells and osteoblastic activity in the cell line KS483. British journal of nutrition. 2008;99(4):715-22.   DOI
3 Yim YK, Jeong YT. Effects of the Herbal-Acupuncture with Acanthopanacis cortex extract at KI10(Eumgok) on osteoporosis in ovariectomized DDY mice. Korean J Acupunct. 2008;25(1):165-96.
4 Tanaka Y, Nakayamada S, Okada Y. Osteoblasts and osteoclasts in bone remodeling and inflammation. Curr Drug Targets Inflamm Allergy. 2005;4:325-8.   DOI
5 So HY, Ahn SH, Song RY, Kim HL. Relationships among obesity, bone mineral density, and cardiovascular risks in postmenopausal women. Korean J Women Health Nurs. 2010;16(3):224-33.   DOI
6 Roodman GD. Regulation of osteoclast differentiation. Ann N Y Acad Sci. 2006;1068:100-9.   DOI
7 William JB, Scott S, David LL. Osteoclast differentiation and activation. Nature. 2003;423:337-42.   DOI
8 Khosla S, Burr D, Cauley J, David WD, Peter RE, Dieter F, et al. Bisphosphonates-associated osteonecrosis of the jaw: report of a task force of the american society for bone and mineral research. J Bone Miner Res. 2007;22:1479-91.   DOI
9 Khosla S, Bilezikian JP, Dempster DW, Lewiecki M, Miller PD, Neer RM, et al. Benefits and risks of bisphosphonate therapy for osteoporosis. J Clin Endocrinol Metab. 2012;97:2272-82.   DOI
10 Korean Endocrine Society. Osteoporosis Fact Sheet 2014. Endocrinol Metab [Internet]. 2014 [cited 2019 Mar 7]. Available from: http://www.endocrinology.or.kr/image/main/kor_Osteoporosis_Fact_Sheet2014.pdf.
11 Raggatt LJ, Partridge NC. Cellular and molecular mechanisms of bone remodeling. J Biol Chem. 2010;285:25103-8.   DOI
12 Kannel KA, Drake MT. Adverse effects of bisphosphonates: implications for osteoporosis management. Mayo Clin Proc. 2009;84:632-8.   DOI
13 Lips P, van Schoor NM. Quality of life in patients with osteoporosis. Osteoporosis International. 2005;16(5):447-55.   DOI
14 Shin JM, Park CK, Shin EJ, Jo TH, Hwang IK. Effects of Scutellaria radix extract on osteoblast differentiation and osteoclast formation. Korean J Food Sci Technol. 2008;6(40):674-9.
15 Jung YT, Choi YH, Song JH, Lee CH, Lee MS, Jang SJ, et al. Effect of water extract of Eucommiae cortex in RANKL-induced osteoclast differntiation. J Physiol & Pathol Korean Med. 2009;23(3):613-8.
16 Lee KS, Choi ES, Han SY, Kim YK. Inhibitory effect of water extracts of Aconiti Lateralis Preaparata Radix and Acanthopanacis Cortex on differntiation of bone marrow-derived adipocytes and osteoclasts. Herb Formula Sci. 2014;22(1):151-65.   DOI
17 Lin CC, Shieh DE. The anti-inflammatory activity of Scutellaria rivularis extracts and its active components, baicalin, baicalein and wogonin. Am J Chin Med. 1996;24:31-6.   DOI
18 Assoication of clinical research. Essential manual for clinical practice. 1st ed. Seoul: Euihakseowon; 2013.
19 The Korean orthopaedic association. Orthopaedics. 7th ed. Seoul: ChoishinEuihaksa; 2016.
20 Hong SB. Treatment and Diagnosis of Osteoporosis. J Korean Neurol Assoc. 2017;35(4):20-4.   DOI
21 Health Insurance Review & Assessment Service. [cited 2019 Mar 7]. Available from: http://opendata.hira.or.kr/op/opc/olap3thDsInfo.do.
22 Karst M, Gorny G, Galvin RJ, Oursler MJ. Roles of stromal cell RANKL, OPG, and M-CSF expression in biphasic $TGF-{\beta}$ regulation of osteoclast differentiation. J Cell Physiol. 2004;200:99-106.   DOI
23 Vaananen HK, Horton M. The osteoclast clear zone is a specialized cell extracellular matrix adhesion structure. J Cell Sci. 1995;108:2729-32.   DOI
24 Duplomb L, Dagouassat M, Jourdon P, Heymann D. Embryonic stem cells: new tool to study osteoblast and osteoclast differentiation. Stem Cells. 2007;25(3):544-52.   DOI