Browse > Article
http://dx.doi.org/10.15188/kjopp.2019.02.33.1.39

Comparison of Ingredient and Efficacy of Galgeun-tang (Gegen-tang) Mix Extract Powder and Decoction  

Kim, Jung Ok (National Development Institute of Korean Medicine(NIKOM))
Kim, Se Jin (National Development Institute of Korean Medicine(NIKOM))
Kim, Jong Beom (National Development Institute of Korean Medicine(NIKOM))
Nam, Won Hee (National Development Institute of Korean Medicine(NIKOM))
Lee, Ji Beom (National Development Institute of Korean Medicine(NIKOM))
Lee, Hwa Dong (National Development Institute of Korean Medicine(NIKOM))
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.33, no.1, 2019 , pp. 39-47 More about this Journal
Abstract
Galgeun-tang (GGT) is a traditional herbal medicine generally used to treat cold, fever, headache, migraine and convulsion. To investigate the difference of ingredient and efficacy of GGT according to production method, mix extract powder and decoction, the quantities of index components, antioxidant and anti-inflammatory effects of two kinds of GGT formulation were compared. The contents of ten index components were simultaneously analyzed with HPLC. A HPLC method was developed and validated for the simultaneous determination. The GGT mix extract powder contained higher puerarin and daidzin content than the decoction, while the GGT decoction contained higher cinnamic acid and glycyrrhizic acid content than the mix extract powder. But ingredient of GGT mix extract powder and decoction was statistically very similar (r=0.8335). GGT decoction showed higher antioxidant effect than GGT mix extract powder, but the difference was low. The patterns of productions for NO, iNOS, COX-2, TNF-${\alpha}$, IL-$1{\beta}$ and IL-6 were decreased along the increasing dose of GGT formulation in LPS-induced RAW 264.7 macrophages. The inhibitory effects on NO, iNOS, COX-2, IL-$1{\beta}$ and IL-6 in Raw 264.7 cell were very similar to each other. The results obtained in this study could be used as fundamental data for verifying the equivalence and effectiveness of the herbal medicines.
Keywords
Galgeun-tang; Simultaneous determination; Antioxidant effect; Anti-inflammatory effect;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Kang KH, Chae BY. The effect of Sagan-tang applied to conjunctivitis on hepatic damage in rats. Kyunghee Univ Oriental Med J. 1985;8(1):185-94.
2 Lee SD, Bai HS, Koo BH. The effects of Samhwangsasimtang on the blood pressure and serum in S.H.R. Kyunghee Univ Oriental Med J. 1984;7(1):181-91.
3 Park HM, Choi KH, Jung JY, Lee SD. Metal exposure through consumption of herbal medicine, and estimation of health risk among Korean population. Kor J Env Health. 2006;32(2):186-91.
4 Kim EJ, Park HJ, Kim HJ, Kim JH, Ann JY, Lee JH, Kim YK. A monitoring study of marker contents in the Hwangnyeonhaedok-tang ex preparations on the market. Korean J Oriental Med Prescrip. 2008;16(1):95-107.
5 Kim JH, Seo CS, Shin HK. The comparative study on decoctions of Ssanghwa-tang (Shuanghe-tang) extracted by different extraction conditions. Korean J Oriental Med Prescrip. 2010;18(2):125-34.
6 Bansky D, Barolet R. Chinese Herbal Medicine Formulas and Strategies. Eastland Press, Seattle; 1990. p. 7-8.
7 Bi Z, Guo J, Zhang S, Li P. Study on the variation of the main constituents in Gardeniae Fructus after compatibility with Scutellariae Radix. Pharm Clini Res. 2011;19:29-31.   DOI
8 Kim NM, Kim DH. Quality change of cinnamon extract prepared with various drying methods. Korean J Food & Nutr. 2000;13:152-7.
9 Kim NM, Sung HS, Kim WJ. Effects of solvents and some extraction conditions on antioxidant activity in cinnamon extracts. Korean J Food Sci Technol. 1993;25(3):204-9.
10 Korea Food and Drug Administration. The Korean Herbal Pharmacopoeia 11th ed; 2016.
11 Blois MS. Antioxidant determinations by the use a stable free radical. Nature. 1958;26:1199-200.   DOI
12 Yang TK, Kim YB, Chae BY. An Experimental study on the anti-allergic effects, anti-inflammatory action, anti-pyretic action and analgesic action of Galgeun-tang, Gamigalgeun-tang and Geomahwanggalgeun-tang. J Orient Med Surgery, Ophthalmology & Otolaryngology. 2002;15(1):76-95.
13 Korea Food and Drug Administration. Regulation on Approval and Notification of Herbal (crude) Medicinal Preparations, Etc. KFDA Notification No. 2016-112; 2016.
14 Korea Food and Drug Administration. Analytical method guideline about validation of drug and etc; 2015.
15 Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid content in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999;64(4):555-9.   DOI
16 Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic -phosphotungstic acid reagents. Am J Enol Vitic. 1965;16(3):144-58.
17 Lee SM, The change of ginsenosides composition from decocted ginseng with citric acid and Schisandrae Fructus. Kor. J. Pharmacogn. 2014;45(2):121-6.
18 Kim SD, Um YR, Yang MC, Yun NY, Jung SW, Lee JH, Ma JY. Equivalence of mixed and individual preparation of Galgeun-tang in terms of polyphenol contents and DPPH radical scavenging activity. Yakhak Hoeji. 2011;55(1):45-8.
19 Yun YG. East Medical Traditional Medical Formulae and prescription Commentary. Seoul: Euiseongdang. 1998. p. 145.
20 Choi SM, Kim BW. Theoretical chemical reaction of herb medicine. J Pharmacopunct. 2002;5(2):116-9.   DOI
21 Lee JK, Kim JH, Shin HK. Systematic Studies on Galgeun-tang (gegen-tang, kakkon-to) for Establishment of Evidence Based Medicine. Kor J Oriental Med Prescrip. 2011;19(1):103-11.
22 Ha HK, Lee JK, Lee MY, Lim HS, Shin HK. Galgeun-tang, an Herbal Formula, Ameliorates Atopic Dermatitis Responses in Dust Mite Extract-treated. NC/Nga Mice. J Korean Med. 2013;34(4):1-11.   DOI
23 Kim YJ, Kim OS, Seo CS, Lim HS, Yoo SR, Jeon WY, Jin SE, Shin IS, Kim JH, Shin NR, Kim SS, Lee MY, Jeong SJ, Ha HK, Sin HK. Study on Comparison of Ingredient Quantities and Biological Activities of Galgeun-tnag according to Extraction Solvant. Kor. J. Oriental Phys & Path. 2012;26(6):908-14.
24 Shin NR, Kim C, Seo CS, Ko JW, Cho YK, Shin HK, Kim JS. Galgeun-tang Attenuates Cigarette Smoke and Lipopolysaccharide Induced Pulmonary Inflammation via $I{\kappa}-B{\alpha}/NF-{\kappa}B$ Signaling. molecules. 2018;23(10):2489-99   DOI
25 Wynn TA, Chawla A, Pollard JW. Macrophage biology in development homeostasis and disease. Nature. 2013;496(7446):445-55.   DOI
26 Kim OS, Seo CS Seo, Kim YJ, Shin HK Shin. Comparative study on chemical composition and biological activities of Samchulkunbi-tang decoction and commercial herbal medicine. Formula Science. 2012;20(2):1-11.