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http://dx.doi.org/10.15188/kjopp.2018.10.32.5.333

Comparison of Marker Components and Biological Activities of Gamiguibi-tang(Jiaweiguipi-tang) Decoction and Commercial Extract Granules  

Kim, Jung Ok (Traditional Korean Medicine Technology Division, National Development Institute of Korean Medicine)
Baek, Ka Yeon (Traditional Korean Medicine Technology Division, National Development Institute of Korean Medicine)
Lee, Hwa Dong (Traditional Korean Medicine Technology Division, National Development Institute of Korean Medicine)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.32, no.5, 2018 , pp. 333-340 More about this Journal
Abstract
Gamiguibi-tang (GGBT) is a traditional herbal medicine generally used to treat anemia, insomnia, anxiety, and nervousness. GGBT is being commercially produced in the form of extract granule and the quality control methods are specified in the Korean Herbal Pharmacopeia (KHP). However, there is no method to simultaneously analyze compound preparations. In this study, a HPLC method was developed and validated for the simultaneous determination of marker compounds in GGBT. And the contents of marker components and biological activities of the commercial GGBT extract granules (GGBT-2 and GGBT-3) were compared with those of the GGBT decoction (GGBT-1). We confirmed the robustness of simultaneous analytical method by monitoring the contents of the commercial GGBT products and carrying out validation. The marker components of GGBT were geniposide ($8.03{\sim}12.70{\mu}g/mL$), paeoniflorin ($2.79{\sim}4.25{\mu}g/mL$) and glycyrrhizic acid ($5.06{\sim}6.30{\mu}g/mL$). DPPH and ABTS radical scavenging activities were 47.34~63.17% and 21.52~33.61% in the GGBT products concentration of $1,000{\mu}g/mL$, respectively. The GGBT products significantly decreased NO, iNOS and COX-2 production in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages in a concentration-dependent manner. The GGBT-2 had higher contents of marker components and biological activities than GGBT-1 and GGBT-3. The research suggest that be used in developing quality control methods for enhancing the quality of herbal medicines.
Keywords
Gamiguibitang(GGBT); Simultaneous determination; Antioxidant effect; Anti-inflammatory effect;
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