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http://dx.doi.org/10.15188/kjopp.2018.02.32.1.13

Antioxidant and Anti-dyslipidemic Effect of Artemisiae iwayomogii Herba, Curcumae longae Radix, and Plantaginis Semen Complex Extract(ACP) on HepG2 Cells  

Jung, Eunsun (Department of Circulatory Internal Medicine, College of Oriental Medicine, Daejeon University)
Cho, Hyun Kyoung (Department of Circulatory Internal Medicine, College of Oriental Medicine, Daejeon University)
Kim, Yoon Sik (Department of Circulatory Internal Medicine, College of Oriental Medicine, Daejeon University)
Yoo, Ho Ryong (Department of Circulatory Internal Medicine, College of Oriental Medicine, Daejeon University)
Seol, In Chan (Department of Circulatory Internal Medicine, College of Oriental Medicine, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.32, no.1, 2018 , pp. 13-23 More about this Journal
Abstract
This study was performed to investigate the antioxidant and antidyslipidemic effects of Artemisiae iwayomogii Herba, Curcumae longae Radix and Plantaginis Semen complex extract(ACP) on HepG2 cells. We measured total polyphenols, total flavonoids, radical scavenging activity, and ABTS radical scavenging activity of ACP to evaluate its antioxidant activity. HepG2 cells were treated with ACP. Then, we evaluated ROS production; intracellular GSH content; GPx, GR, SOD, and catalase activities; free fatty acids and MDA levels; and mRNA expression levels of ACAT1 and HMG-CoA reductase. Results: ACP contains polyphenols and flavonoids and increased the DPPH and ABTS radical scavenging activities in HepG2 cells in a dose dependent manner. Also, ACP significantly reduced ROS production in HepG2 cells compared to the control group and significantly increased the GSH content, and elevated the enzyme activities of GPx, GR, and catalase in HepG2 cells compared to the control group. In addition, ACP reduced the mRNA expression of ACAT1 and HMG-CoA reductase in HepG2 cells compared to that in the control group. Conclusion: These results suggest that ACP has an antioxidant effect and may suppress the expression of dyslipidemia - associated genes and thus may be useful for the improvement of dyslipidemia.
Keywords
Dyslipidemia; Anti-oxidant; Artemisiae iwayomogii Herba; Curcumae longae Radix; Plantaginis Semen;
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