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http://dx.doi.org/10.15188/kjopp.2016.10.30.5.308

Effects of Oja-Shingiwhan in Contracted Corpus Cavernosum Smooth Muscle  

Park, Jeong Su (National Evidence-based Healthcare Collaborating Agency)
Ahn, Sang Hyun (Department of Anatomy, College of Korean Medicine, Semyung University)
Park, Sun Young (Department of Physiology, College of Korean Medicine, Semyung University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.30, no.5, 2016 , pp. 308-313 More about this Journal
Abstract
The purpose of this study is to investigate the effects of Oja-Shingiwhan(OS) in contracted corpus cavernosum smooth muscle and its mechanism. To evaluate the relaxation of OS in contracted corpus cavernosum, OS was treated in strips which were precontracted with phenylephrine(PE). To examine its mechanism, OS was treated into corporal strips contracted by PE after pretreatment of Nω-nitro-L-arginine(L-NNA) and compared with non-pretreatment of L-NNA. In calcium chloride(Ca2+)-free krebs solution, Ca2+ 1 mM was treated into corporal strips contracted by PE after pretreatment of OS and compared with non-pretreatment of OS. action were measured by histochemical, immunohistochemical methods. OS significantly affected on the relaxation of corporal strips, and the relaxation effects were inhibited by pretreatment of L-NNA. Contractions induced by Ca2+ influx were inhibited by pretreatment of OS in Ca2+-free krebs solution. OS increased eNOS positive reaction in corpus cavernosum, but decreased PDE-5 positive reaction. These result suggest that the effect of OS in contracted corpus cavernosum smooth muscle are shown by suppressing extracellular Ca2+ influx and increase of eNOS, NO production and decrease of PDE-5.
Keywords
Oja-Shingiwhan; Corpus Cavernosum; Nitric oxide; eNOS; PDE5;
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