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http://dx.doi.org/10.15188/kjopp.2016.08.30.4.242

Effects of Prunellae Herba on the H2O2-Treated LLC-PK₁ Cell's Redox Status and NF-κB Signaling  

Son, Jong Seok (Department of Internal Medicine, Colleage of Korean Medicine, Dongguk University)
Jeong, Ji Cheon (Department of Internal Medicine, Colleage of Korean Medicine, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.30, no.4, 2016 , pp. 242-249 More about this Journal
Abstract
This study was to investigate the anti-inflammatory effects of Prunellae Herba(PH). The generation of superoxide anion radical (․O2-), nitric oxide (NO), peroxynitrite (ONOO-) and Prostaglandin E₂(PGE2) were measured in the H2O2-Treated renal epithelial cells(LLC-PK1 cell) of mouse. And the effects of Prunellae Spica on the expression of NF-κB (p50, p65), IKK-α, phospho-IκB-α and inflammation-related proteins, COX-2, iNOS, IL-1β and VCAM-1, were examined by western blot. The fluorescent probes, 2',7'-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihyldrorhodamine 123 (DHR 123) were used to estimate the scavenging effect of Prunellae Spica on ․O2-, NO, ONOO-. Western blot was conducted to assess the protein expression levels of NF-κB (p50, p65), IKK-α, phospho-IκB-α, inflammation-related proteins, COX-2, iNOS, IL-1β, VCAM-1. PH inhibited H2O2-treated cell death dose-dependently. It reduced the generation of ·O2-, NO, ONOO- and PGE₂ in the H2O2-treated renal epitheial cells(LLC-PK1 cell) of mouse in vitro. PH reduced the expression of NF-κB, IKK-α, phospho-IκB-α, COX-2, iNOS, IL-1β and VCAM-1 genes through means of decreasing activation of NF-κB signaling as well. According to these results, PH has an antioxidative activity and anti-inflammatory effect by regulating the NF-κB pathway. This suggest that PH is expected to be used to regulating inflammatory process and treating inflammation-related disease.
Keywords
Prunellae Herba; Reactive oxygen species; Reactive nitrogen species; NF-κB; Redox Status; anti-inflammatory;
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1 Osborn, L., Hession, C., Lizard, R., Vassallo, C., Luhowskyj, S., Chi-Rosso, G., et al. Direct Expression Cloning of Vascular Cell Adhesion Molecule 1, a Cytokine-Induced Endothelial Protein That Binds to Lymphocytes. Cell. 59: 1203-1211, 1989.   DOI
2 Chung, H.Y., Kim, H.j., Kim, J.W. The inflammation hypothesis of aging: Molecular modulation by calorie restriction. Ann N Y Acad Sci. 928: 327-335, 2001.   DOI
3 Kim, H.J., Kim, K.W., Yu, B.P., Chung, H.Y. The effect of age on cyclooxygenase-2 gene expression: NF-κB activation and IκBα degradation. Free Radic Biol Med. 28(5):683-692, 2000.   DOI
4 Crow, J.P., Beckman, J.S., McCord, J.M. Sensitivity of the essential zinc-thiolate moiety of yeast alchol dehydrogenase to hypochlorite and peroxynitrite. Biochemistry. 34(11):3533-3552, 1995.   DOI
5 DiDonato, J.A., Hayakawa, M., Rothwarf, D.M., Zandim E., Karin, M. A cytokine-responsive IkB kinase that activates the transcriptional factor NF-kB. Nature. 388: 548-554, 1997.   DOI
6 Lin, A., Karin, M. NF-kB in cancer: a marked target. Semin Cancer Biol. 13: 107-114, 2003.   DOI
7 Li, Q., Van Antwerp, D., Mercurio, F., Lee, K.F., Verma, I.M. Severe liver degeneration in mice lacking the IkappaB kinase 2 gene. Science. 284: 321-325, 1999.   DOI
8 Greten, F.R., Eckmann, L., Greten, T.F., Park, J.M., Li, J.W., Egan, L.J., et al. IKKbeta links inflammation and tumorigenesis in a mouse model of colitis-associated cancer. Cell. 118: 285-296, 2004.   DOI
9 Duyao, M.P., Kessler, D.J., Spicer, D.B., Bartholomew, C., Cleveland, J.L., Siekevitz, M., et al. Transactivation of the c-myc promoter by human T cell leukemia virus type 1 tax is mediated by NF-κB. J Biol Chem. 267: 16288-16291, 1992.
10 Chung, H.Y., Kim, H.J., Kim, K.W., Choi, J.S., Yu, B.P. Molecular inflammation hypothesis of aging based on the anti-aging mechanism of calorie restriction. Micro Res Techinoq. 59: 264-272, 2002.   DOI
11 Baeuerle, P.A., Henkei, T. Function and activation of NF-κB in the immune system. Annu Rev Immunol. 12: 141-179, 1994.   DOI
12 Cha, J.H., Kim, Y.S., Lee, E.M. Effects of Prunellae Spica Water Extract on Immune Response in Macrophage Cells. J Korean Obstet Gyneco. 23(3):91-100, 2010.
13 Kim, N.S., Shon, M.S., Kim, G.N., Hwang, Y.I. Anti-obese and Antioxidant activities of Spica prunellae Extract in 3T3-L1 and HepG2 cells. Food Eng. Prog. 18(4):413-418, 2014.   DOI
14 Hong, E.S., Ahn, G.W., Jo, B.K. The Study on the Potential Anti-aging Properties of Prunella vulgaris Extract In Vitro and In Vivo. J. Soc. Cosmet. Scientists Korea. 34(2):129-135, 2008.
15 Tada, H., Shiho, O., Kuroshima, K., Koyama, M., Tsukamoto, K. An improved colorimetric assay for interleukin 2. J Immunol Methods. 93(2):157-165, 1986.   DOI
16 Nagata, N., Momose, K., Ishida, Y. Inhibitory effects of catecholamines and anti-oxidants on the fluorescence reaction of 4,5-diaminofluorescein, DAF-2, a novel indicator of nitric oxide. J Biochem Tokyo. 125: 658-661, 1999.   DOI
17 Halliwell, B., Gutteridge, J.M.C. Free radicals in Biology and Medicine 3rd edition. Oxford. pp 246-350, 1999.
18 Ryu, S.N., Han, S.J., Park, S.Z., Kim, H.Y. Antioxidative activity and varietal difference of cyanidin 3-glucoside and peonidin 3-glucoside contents in pigmented rice. Kor J. Crop Sci. 45(4):257-260, 2000.
19 Alfadda, A.A., Sallam, R.M. Reactive oxygen species in health and disease. J Biomed Biotechnol. 2012: 936486, 2012.   DOI
20 Cathcart, R., Schwiers, E., Ames, B.N. Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein fluorescent assay. Anal Biochem. 134: 111-116, 1983.   DOI
21 Kooy, N.W., Royall, J.A., Ischiropoulos, H., Beckman, J.S. Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic Res Commun. 16: 149-156, 1994.   DOI
22 Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. Protein measurement with the Folin phenol reagent. J Biol Chem. 193(1):265-275, 1951.
23 Rubin, E., Reisner, H.M. Essentials of Rubin's Pathology. KOONJA. pp 7-8, 2013.
24 Carr, A.C. Oxidation of LDL by myeloperoxidase and reactive nitrogen species: reaction pathways and antioxidant protection. Arterioscler Thromb Vasc Biol. 20: 1716-1723, 2000.   DOI
25 Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T., Mazur, M., Telser, J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39: 44-84, 2007.   DOI
26 Chae, S.W. Function and Activation of NF-κB in Immune System. Korean J Otolaryngol 48: 284-288, 2005.
27 Golan, D.E., Tashjian, A.H., Armstrong, E.J., Armstrong, A,W. Principles of Pharmacology: The Pathophysiologic Basis of Drug Theraphy, Second Edition. EPUBLIC. pp 743-750, 2008.
28 Lee, M.S., Kim, K.A. NF-κB Pathway in Metabolic/ endocrine Diseases. Endocrinol Metab. 21(5):352-363, 2006.
29 Seo, B.I., Kim, S.H. The effects of Prunellae Spica on immune response and anti-allergic reaction. J. of Herbology. 12(1):7-24, 1997.
30 University of Korean Medicine Joint Publication Committee. Bonchohak. Yeongnimsa. pp 209-210, 2007.
31 Chang, H.J., Park, S.J., Lee, J.R., Kim, S.C. Inhibitory Effects of Water Extracy of Prunellae Spica on the Production of Pro-inflammatory Mediator in LPS-activated Raw 264.7 Cells. Korean J. Oriental Physiology & Pathology. 23(3):599-607, 2009.
32 Heo, J.K., Lee, J.M., Lee, C.H., Cho, J.H., Lee, K.S. Effect of Prunellae Spica Extracts on Anti-tumor Metastasis by Immune Activity. J Korean Obstet Gyneco. 22(1):41-52, 2009.
33 Im, S.B.N., Lee, H.J. Effects of Labiatae Herba Prunellae Aqua-acupuncture in Hyperthyroidism Rats. The Journal of Korean Oriental Medical Society. 18(1):399-410, 1997.
34 Heo, J.K., Hwang, D.S., Lee, J.M., Lee, C.H., Jang, J.B., Lee, K.S. Inhibitory Effects of Ursolic Acid from Prunella Vulgaris on Osteoclast Differentiation. J Korean Obstet Gyneco. 27(2):59-70, 2014.   DOI