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http://dx.doi.org/10.15188/kjopp.2016.04.30.2.101

Mechanism of Corni Fructus Induced Vasorelaxation in Rabbit Carotid Artery  

Kim, Hyung Jun (Department of Gynecology, College of Korean Medicine, Semyung University)
Park, Sun Young (Department of Physiology, College of Korean Medicine, Semyung University)
Kim, Tae Yeon (Department of Preventive Medicine, College of Korean Medicine, Semyung University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.30, no.2, 2016 , pp. 101-108 More about this Journal
Abstract
This study is conducted to investigate vasorelaxant effect of Corni Fructus(CF) on rabbit carotid artery. To determine vasorelaxant effect of CF on rabbit carotid artery, arterial sections with intact or removed endothelium were used in this organ bath study. After being contracted by phenylephrine(PE), arterial sections were treated with CF extract in a dose-dependent manner. To identity its mechanism, the contracted arterial sections by PE were pretreated with indomethacin(IM), tetraethylammonium chloride(TEA), Nω-nitro-L-arginine(L-NNA) or methylene blue(MB) and 1.0 ㎎/㎖ CF extract. We also studied to confirm the effect on influx of extracellular calcium chloride(Ca2+) of the CF extract in rabbit carotid artery. To measure the cytotoxicity of the CF extract, cell viability of human umbilical vein endothelial cell(HUVEC) was measured by MTT assay. Generation of nitric oxide(NO) was also measured by Griess reagent. The arterial sections with intact endothelium were relaxed significantly by CF extract, but this effect was inhibited in the arterial sections with damaged endothelium. The vasorelaxant effect was inhibited significantly when arterial sections were pretreated with IM, TEA, L-NNA, MB. In Ca2+-free krebs solution, increasing of arterial contraction by Ca2+ was also inhibited by CF significantly. The treatment of CF extract increased NO concentration in HUVEC. This study suggested that the vasorelaxant effect of CF extract would be related with endothelium derived relaxing factor(EDRF) such as NO, prostacyclin(PGI2), endothelium derived hyperpolarization factor(EDHF).
Keywords
Corni Fructus(CF); Vasorelaxant effect; Organ bath study; Nitric oxide(NO);
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