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http://dx.doi.org/10.15188/kjopp.2016.02.30.1.20

Effect of Prunellae Spica on Oxidative Stress and Mitochondrial Dysfunction in the Hepatocyte  

Jang, Mihee (Medical Research Center (MRC-GHF), College of Korean Medicine, Daegu Haany University)
Seo, Hye Lim (Medical Research Center (MRC-GHF), College of Korean Medicine, Daegu Haany University)
Kim, Sang Chan (Medical Research Center (MRC-GHF), College of Korean Medicine, Daegu Haany University)
Kim, Young Woo (Medical Research Center (MRC-GHF), College of Korean Medicine, Daegu Haany University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.30, no.1, 2016 , pp. 20-26 More about this Journal
Abstract
Prunellae Spica, the herbaceous plant in the genus Prunella, is a traditional herbal medicine and has been reported to have diuretic, anti-bacterial and anti-oxidant effects. However, the mechanism of its action was not clearly identified. In the present study, we investigated the hepatoprotective effect of Prunellae Spica extract (PSE) against the damage of mitochondria and death in hepatocyte induced by oxidative stress. Treatment of arachidonic acid (AA)+iron significantly induced oxidative stress and apoptosis in the hepatocytes. However, PSE protected cells and inhibited apoptosis by altering the protein levels such as poly(ADP-ribose) polymerase and pro-caspase 3. Moreover, AA+iron induced reactive oxygen species production and mitochondrial dysfunction, and Both of them were inhibited by PSE treatment. PSE markedly activated AMP-activated protein kinase (AMPK), an important regulator in cell survival. Furthermore, this activation by PSE was mediated with liver kinase B1, a major upstream kinase that phosphorylates Thr 172 of AMPKα, and this activation was associated with its cell protection, as assessed by an experiment of a chemical inhibitor. In conclusion, this study demonstrate that PSE protects hepatocytes against oxidative stress as mediated with activation of LKB1-dependent AMPK pathway.
Keywords
Prunellae Spica; Oxidative Stress; Mitochondria; Liver; AMPK;
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