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http://dx.doi.org/10.15188/kjopp.2015.02.29.1.58

Protective Effect of Fermented Red Ginseng Extracts on Photoaging Skin of Induced by UVB in Hairless Mice  

Kim, Ho Il (Department of Anatomy, College of Korean Medicine, Woosuk University)
Oh, Mi Jin (College of Food & Biotechnology, Woosuk University)
Kim, Jong Seok (Department of Rehabilitation Studies, Woosuk University)
Lee, Sung Cheol (Department of Sports Medicine, Woosuk University)
Kwon, Jin (Department of Prosthetics and Orthotics, Korea National College of Welfare)
Lee, Chang Hyun (Department of Anatomy, College of Korean Medicine, Woosuk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.29, no.1, 2015 , pp. 58-65 More about this Journal
Abstract
This study is designed to investigate the protective effects of fermented Red Ginseng (FRG) against photoaging in vitro and in vivo. UVB was irradiated to the human keratinocyte HaCaT cell and dorsal skin of SKH-1 mice for the induction of photoaging. After treatment of non-fermented red ginseng (NRG), fermented red ginseng (FRG), and fortified fermented red ginseng (FFRG) to the UVB irradiated HaCaT cell, ROS production and activity of MMP-9 were examined by DCFC-DA assay and gelatin zymographic assay respectively. UVB irradiated SKH-1 mice were treated with NRG, FRG, and FFRG via oral(300 mg/Kg B.W./day) and topical($100{\mu}{\ell}/mouse/day$) route.All of NRG, FRG, and FFRG had significantly reduced the intracellular ROS production elicited by UVB, among them FRG slightly more reduced the ROS production than NRG and FFRG. FFRG had slightly more reduced the MMP-9 activity in UVB irradiated HaCaT cells than NRG and FFRG in high dose. Oral and topical treatment of NRG, FRG, and FFRG had decreased the expression of matrix metalloproteinase-2, -3, and -9 in dorsal skin of UVB irradiated mice. Among them, inhibitory effect of FRG on the expression of MMP-2 was apparent. We speculate that FRG has therapeutic potentials on the UVB irradiated photoaging.
Keywords
fermented red ginseng; photoaging skin; HaCaT cells; MMP; ROS;
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1 Lee, J., Jung, E., Lee, J., Sungran, H., Kim, J., Park, M., So, J., Ham, Y., Jung, K., Hyun, C.G., Kim, Y.S., Park, D., Panax ginseng induces human type I collagen synthesis through activation of Smad signaling. J Ethnopharmacol 109: 29-34, 2007.   DOI   ScienceOn
2 Masak, H., Atsumi, T., Sakurai, H. Detection of hydroxygen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation. Biochem Biophys Res Commun 206: 474-479, 1995.   DOI   ScienceOn
3 Pinkus, R., Weiner, L.M., Daniel, V. Role of oxidants and antioxidants in the induction of AP-1, NF-κB, and glutathione S-transferase gene expression. Biol Chem 271(23):13422-13429, 1996.   DOI   ScienceOn
4 Odani, T., Tanizawa, H., Takino, Y. Studies on the absorption, distribution, excretion and metabolism of ginseng saponins. II. The absorption, distribution and excretion of ginsenoside Rg1 in the rat. Chem Pharm Bull 31: 292-298, 1983.   DOI   ScienceOn
5 Shin, Y.S. Fermentation of Red Ginseng using CKDHC0801 and CKDHC0802. Korean J Food Cookery Sci 26: 469-474, 2010.
6 Birkedal-Hansen, H. Proteolytic remodeling of extracellular matrix. Curr Opin Cell Biol 7: 728-735, 1995.   DOI   ScienceOn
7 Kligman, A.M., Grove, G.L., Hirose, R., Leyden, J.J. Topical tretinoin for photoaged skin. J Am Acad Dermatol 15: 836-859, 1986.   DOI   ScienceOn
8 Reynolds, J.J. Collagenases and tissue inhibitors of metalloproteinases: a functional balance in tissue degradation. Oral Dis 2: 70-76, 1996.   DOI
9 Kim, Y.G., Sumiyoshi, M., Sakanaka, M., Kimura, Y.. Effects of ginseng saponins isolated from red ginseng on ultraviolet B-induced skin aging in hairless mice. Eur J Pharm 602:148-156, 2009.   DOI   ScienceOn
10 Kim, N.M., Koo, B.S., Lee, S.K., Hwang, E.I., So, S.H., Do, J.H. Effect of Korea red ginseng on collagen biosynthesis and MMP-1 activity in human dermal fibroblast. J Ginseng Res 31(2):86-92, 2007.   DOI   ScienceOn
11 Shindo, Y., Wit. E., Packer, L. Antioxidants defense mechanisms in murine epidermis and dermis and their responses to ultraviolet light. J Invest Dermatol 100: 260-265, 1993.   DOI   ScienceOn
12 Sander, C.S., Chang, H., Salzmann, S., Müller, C.S., Ekanayake-Mudiyanselage, S., Elsner, P., Thiele, J.J. Photoaging is associated with protein oxidation in human skin in vivo. J Invest Dermatol 118(4):618-625, 2002.   DOI   ScienceOn
13 Ha, K.T., Kim, J.K., Lee, Y.C., Kim, C.H. Inhibitory effect of Daesungki-Tang on the invasiveness potential of hepatocellular carcinoma through inhibition of matrix metalloproteinase-2 and -9 activities. Toxicol Appl Pharmacol 200(1):1-6. 2004.   DOI   ScienceOn
14 Landau, M. Exogenous factors in skin aging. Curr Probl Dermatol 35:1-13, 2007.
15 Berneburg, M., Plettenberg, H., Krutmann, J. Photoaging of human skin. Photodermatol Photoimmunol Photomed 16: 239-244, 2000.   DOI   ScienceOn
16 Kiss, I., Chen, S., Tramposch, K.M. The effect of high and low ultraviolet-B dose exposure on the degree of hairless mouse skin wrinkling. Photochem Photobiol 53: 109-112, 1991.   DOI   ScienceOn
17 Kim, D.H. Ginseng and health. Hyoil, 2005.
18 Kim, C.H., Shin, M.K., Ann, D.K., Lee, K.S. Dictionary of chinese herbal medicine, Chungdam, 1997.
19 Ann, D.K. Effects of Ginseng by properties-flavours theory. J Herbology 7(1):27-32, 1992.
20 Noh, S.H. Effects of Ginseng. J Herbology 7(1):53-60, 1992.
21 Kong, B.M., Park, M.J., Min, J.W., Kim, H.B., Kim, SH., Kim, S.Y., Yang, D.C. Physico-chemical characteristics of white fermented and red ginseng extracts. J Ginseng Res 32: 238-243, 2008.   DOI   ScienceOn
22 Lee, M.J., Won, C.H., Lee, S.R., Kim, J.S., Oh, I.G., Hwang, E.I., Kim, N.M., Kang, B.C., Chung, J.H. Oral administration of KTNG0345 prepared from red ginseng extracts reduces UVB-induced skin wrinkle formation in hairless mice. J Ginseng Res 32(1):48-56, 2008.   DOI   ScienceOn
23 Choi, Y.K., Su, J.U., Park, S.K., Yu, S.N., Kim, S.H., Rhee, M.S., Ahn, S.C., Shin, M.S. Research trends of fermented medicinal herbs - Based on their clinical efficacy and safety assessment. J Life Science 22: 1729-1739, 2012.   DOI   ScienceOn
24 Lee, J.H., Lee, B.S., Yang, M.S., Byun B.S., Kim, W.G., Kim, B.H., Lee, S.J. Prevention of photoaging and wrinkle formation in Hairless mice dorsal skin by APB-03. Food Science and Biotechnology 37(6):989-996, 2005.
25 Kang, T.H., Park, H.M., Kim, Y.B., Kim, H.A., Kim, N.M., Do, J.H., Kang, C.H., Cho, Y.H., Kim, S.Y. Effects of red ginseng extract on UVB irradiation-induced skin aging in hairless mice. J Ethnopharmacol 123: 446-451, 2009.   DOI   ScienceOn
26 Jeong, D.W., Hong, J.W., Shin, W.J., Park, Y.M., Jung, J.H., Kim, C.H., Min, I.K., Park, S.U., Jung, W.S., Park, J.M., Go, C.N., Cho, K.H., Moon, S.K. Effects of Korean ginseng, Korean red ginseng and fermented Korean red ginseng on cerebral blood flow, cerebrovascular reactivity, systemic blood pressure and pulse rate in humans. J Korean Orient Med 27: 38-50, 2006.
27 Meinhard, W., Iiana, T.B., Lale, N., Wenjian, M., Lars, A.S., Ziba, R.W., Jutta, S., Karin, S.K. Solar UV irradiation and dermal photoaging. J Photochem Photobiol 63: 41-51, 2001.   DOI   ScienceOn
28 Hsu, S.M., Raine, L., Fanger, H. Use of avidin-biotin-peroxidase complex(ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody(PAP) procedures. J Histochem Cytochem 29: 577-580, 1981.   DOI   ScienceOn
29 Kehrer, J.P. Free radicals as mediators of tissue injury and disease. CRC Crit Rev Toxicol 23: 21-28, 1993.   DOI
30 Aruoma, O.I. Nutrition and health aspects of free radicals and antioxidant. Food Chem Toxicol 62: 671-683, 1994.   DOI   ScienceOn
31 Lavker, R.M. Structural alterations in exposed and unexposed aged skin. J Invest Dermatol 73: 59-66, 1979.   DOI   ScienceOn
32 Gilchrest, B.A. Skin aging and photoaging: an overview. J Am Acad Dermatol 989(21):610-613, 1989.   DOI   ScienceOn
33 Griffiths, C.E.M. The clinical identification and quantification of photodamage. Br J Dermatol 127(Suppl. 41):37-42, 1992.   DOI   ScienceOn
34 Chung, J.H., Seo, J.Y., Choi, H.R., Lee, M.K., Youn, C.S., Rhie, G., Cho, K.H., Kim, K.H., Park, K.C., Eun, H.C. Modulation of skin collagen metabolism in aged and photoaged human skin in vivo. J Invest Dermatol 117: 1218-1224, 2001.   DOI   ScienceOn
35 Gilchrest, B.A. A review of skin ageing and its medical therapy. Br J Dermatol 135: 867-875, 1996.   DOI   ScienceOn
36 Herrmann, G., Wlaschek, M., Lange, T.S., Prenzel, K., Goerz, G., Scharffetter-Kochanek, K. UVA irradiation stimulates the synthesis of various matrix-metalloproteinases(MMPs) in cultured human fibroblasts. Exp Dermatol 2: 92-97, 1993.   DOI   ScienceOn
37 Jin, Y.R., Yu, J.Y., Lee, J.J., You, S.H., Chung, J.H., Noh, J.Y., Im, J.H., Han, X.H., Kim, T.J., Shin, K.S., Wee, J.J., Yun, Y.P. Antithrombotic and antiplatelet activities of Korean red ginseng extract. Basic Clin Pharmacol Toxicol 100: 170-175, 2007.   DOI   ScienceOn
38 Koivukangas, V., Kallioinen, M., Autio-Harmainen, H., Oikarinen, A. UV irradiation induces the expression of gelatinases in human skin in vivo. Acta Derm Venereol 74: 279-282, 1994.