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http://dx.doi.org/10.17208/kjopp.2014.12.28.6.621

Genotoxicity Study of GST Extract  

Lee, Chul Wha (Division of Respiratory System, Department of Internal Medicine, College of Korean Medicine, Daejeon University)
Han, Jong Min (Division of Respiratory System, Department of Internal Medicine, College of Korean Medicine, Daejeon University)
Lee, Mi Young (Biotoxtech)
Jung, In Chul (Department of Neuropsychology, College of Oriental Medicine, Daejeon University)
Jin, Mirim (Department of Pharmacology, College of Oriental Medicine, Daejeon University)
Kim, Seung Hyung (Institute of Traditional Medicine and Bioscience, Daejeon University)
Park, Yang Chun (Division of Respiratory System, Department of Internal Medicine, College of Korean Medicine, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.28, no.6, 2014 , pp. 621-629 More about this Journal
Abstract
This study aimed to evaluate the genotoxicity of GST (Gamisasangja-tang). For examining genotoxicity, we carried out bacterial reverse mutation assay, chromosome aberration assay, micronucleus induction test according to OECD guidelines. Bacterial reverse mutation assay: In GST treating group, regardless of existence S9 mix, revertant colonies counts appeared to be less than twice of negative control group and dose dependent increase. In positive control group, revertant colonies counts were shown to be more than twice of negative control croup. Chromosome aberration assay: All cell line showed repetition rate of abnormal chromosome aberration less than 5%, regardless of treating time, existence of S9 mix, and no significant change ($p{\succeq}0.05$) compared with negative control group. Micronucleus induction test: Micronucleated polychromatic erythrocytes (MNPCE) repetition rate of Polychromatic erythrocytes (PCE) showed no significant changes compared with negative control group ($p{\succeq}0.05$). PCE portion of total erythrocytes also showed no significant changes ($p{\succeq}0.05$). Our results showed that GST didn't induce any genotoxicity.
Keywords
Sasangja-tang (Shechuangzi-tang); genotoxicity; mutagenicity; chromosome aberration assay; micronucleus induction;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Bieber, T. Atopic dermatitis. N Engl J Med 358(14):1483-1494, 2008.   DOI   ScienceOn
2 Park, Y.M. Epidemiologic Study and Risk Factor of Atopic Dermatitis. Pediatric Allergy Respir Dis 21(2):74-77, 2011.   DOI
3 Kim, T.Y. Lectures of Medical Science: Treatment of Atopic Dermatitis: an Update and Review of the Literature. Pediatric Allergy Respir Dis 19(3):209-219, 2009.
4 Park, Y. Status of clinical practice on diagnosis and management of atopic dermatitis in Korea: a questionnaire survey of physicians. Allergy Asthma Respir Dis 1(3):257-265, 2013.   DOI
5 Chen, S. Wai-ke-zheng-zong. Beijing, People's medical publishing house. p 244, 1964.
6 Gam, B.S., Kim, I.R., Kim, H.C., Guk, Y.B., Park, Y.G., Seo, B.I., Seo, Y.B., Song, H.J., Shin, M.G., Lee, Y.J., Lim, G.H., Jo, S.I., Jung, J.G., Ju, Y.S., Choi, H.Y. Bon-cho-hak. Seoul, Younglimsa. pp 197, 227, 308, 517, 631-633, 1991.
7 Park, Y.C., Kim, S.H., Jin, M.R. Composition comprising the extract of complex herb an active ingredient for preventing and alleviating allergic or non-allergic skin disease and the use thereof. Korea Patent No. 10139796100000. 2014.
8 OECD. OECD guideline testing of chemicals No. 471 Bacterial Reverse Mutation Test. 2001.
9 OECD. OECD guideline testing of chemicals No. 473 in vitro Mammalian Chromosome Aberration Test. 2001.
10 OECD. OECD guideline testing of chemicals No. 474 Mammalian Erythrocyte Micronucleus Test. 2001.
11 Biotoxtech, Standard Operating Procedures (SOP), SOP/GNT/004; Manufacture of positive control material.
12 Sofuni, T. Data book of chromosomal aberration test invitro, Revised edition. Tokyo: Life-Science Information Center; 1998.
13 Fisher, R.A. On the interpretation of x2 from contingency tables, and the calculation of P. Journal of the Royal Statistical Society 85(1):87-94, 1922.   DOI   ScienceOn
14 Chungcheongbukdo Institute of Health Environment. Quality standard for drinking water, water quality criteria. [serial online] 2013 Nov-2014 Feb;[cited 2014 Feb 1] Availed from: URL:http://here.cb21.net/home/sub.do?menu_key=44.
15 Berchtold, W. Comparison of the Kastenbaum-Bowman test and Fisher's exact test. Arch Genet 48(2-3):151-157, 1975.
16 Bartlett, M.S. Properties of sufficiency and statistical test. Journal of the Royal Statistical Society A160:268-282, 1937.
17 Anscombe, F.J. The validity of Comparative Experiments. Journal of the Royal Statistical Society A111(3):181-211, 1948.   DOI
18 Hwang, S.Y., Lee, J.R., Kim, S.C., Jee, S.Y. A study on Genotoxicity Test of Hyeong-gae-yeon-gyo-tang extract. Kor. J. Herbology 22(4):287-300, 2007.
19 Ministry of Food and Drug Safety. Guidelines for toxicity tests of drugs and related materials notification. Chungbuk, MFDS. 2014.
20 Cho, J.B., Lee, J.H., Suh, J.M., Yu, J.S., Lee, H.S., Park, E.A., Kim, H.M., Chang, E.Y., Kim, J.H., Han, Y.S., Ahn, K.M. Change in Quality of Life according to the Change in Atopic Dermatitis Severity. Pediatric Allergy Respir Dis 22(1):86-99, 2012.   DOI
21 Han, J.M., Hong, J.H., Lee, H.Y., Jung, I.C., Jin, M.R., Kim, S.H., Park, Y.C. Single Oral Dose Toxicity Test and Four Weeks Repeated Oral Dose Determinating Test of GST in Sprague-Dawley Rats. J. Int. Korean Med 34(4):349-361, 2013.