Browse > Article

Effects of Bojungchiseub-tang on the Production of Inflammatory Adipokine and MAPK Signaling in 3T3-L1 Mature Adipocytes  

Lee, Soo-Jung (Department of Internal Medicine, College of Korean Medicine, Dong-Eui University)
Kim, Won-IL (Department of Internal Medicine, College of Korean Medicine, Dong-Eui University)
Kang, Kyung-Hwa (Department of Physiology, College of Korean Medicine, Dong-Eui University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.28, no.5, 2014 , pp. 486-493 More about this Journal
Abstract
Adipocytes are endocrine cells that release bioactive mediators called adipokines. In condition of obesity characterized by low-grade chronic inflammation, adipocytes release inflammatory adipokines, which is related to insulin resistance. Bojungchiseub-tang (BJCST) has been used in symptoms and signs of edema, dampness-phlegm, kidney failure, and so on in Korean medicine. BJCST is also expected to have anti-obesity activities. In the present study, we examined whether BJCST modulate the production of inflammatory adipokines and the activations of the mitogen-activated protein kinases (MAPK) signaling pathway related to induce adipocyte inflammation to elucidate the effects and its mechanism of BJCST on lowering the content of inflammatory adipokines in 3T3-L1 adipocytes. As a result, BJCST suppressed the production of proinflammatory cytokines, tumor necrosis factor (TNF) $-{\alpha}$, interleukin (IL) $-1{\beta}$, IL-6, interferon (IFN) -${\gamma}$, granulocyte-macrophage colony-stimulating factor (GM-CSF), monocyte chemoattractant protein-1 (MCP-1), and the production of other inflammatory mediators, prostaglandin $E_2(PGE_2)$ and nitric oxide(NO)viadownregulationofcyclooxygenase-2(COX-2)andinducible NO synthase (iNOS) gene expressions. In addition, BJCST decreased the phosphorylation of MAPK that promotes the production of inflammatory adipokines in 3T3-L1 mature adipocytes. In conclusion, BJCST could regulate the production of inflammatory adipokines and MAPK signaling pathway related to induction of adipose inflammation.
Keywords
Bojungchiseub-tang (BJCST); obesity; 3T3-L1 adipocyte; adipokines; mitogen-activated protein kinases (MAPK) signaling;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Jaworski, K., Ahmadian, M., Duncan, R.E., Sarkadi-Nagy, E., Varady, K.A., Hellerstein, M.K., Lee, H.-Y., Samuel, V.T., Shulman, G.I., Kim, K.-H., de Val, S., Kang, C., Sul, H.S. AdPLA ablation increases lipolysis and prevents obesity induced by high fat feeding or leptin deficiency. Nat Med. 15(2):159-168, 2009.   DOI   ScienceOn
2 Rocha, V.Z., Folco, E.J., Sukhova, G., Shimizu, K., Gotsman, I., Vernon, A.H., Libby, P. Interferon-$\gamma$, a Th1 Cytokine, Regulates Fat Inflammation A Role for Adaptive Immunity in Obesity. Circ Res. 103(5):467-476, 2008.   DOI
3 Kanda, H. MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity. J Clin Invest. 116(6):1494-1505, 2006.   DOI   ScienceOn
4 Du H.G. Dong-Yi Singyehak. Seoul, Dongyangeuihakyeonguwon. pp 7-9, 371-372, 374-375, 369, 393, 449, 1993.
5 Kim S.Y. Effects of the Extracts from Atractylodes Rhizoma on obese rats and Differentiation of 3T3-L1 Cells. Wonkwang University Master's thesis. 1997.
6 Shin I.S. The mixture of Citri Pericarpium and Lonicerae Flos improves obesity through regulations of peripheral lipid metabolism and central neuroendocrine in mice fed a high fat diet. Daegu Haany University Master's thesis. 2012.
7 Hwang J.S., Suk J.M., Choi H.M., Shin I.S., Hwang S.J., Park J.Y., Kim S.O., Seo B.I., Kim M.R., Effects of Plantaginis Semen & Poria (CJB) Water Extract on Histomorphometrical Aspects and Histopathological Changes in High Fat Diet-induced Obese Rats Kor. J. Herbology 28(1):91-96, 2013.   과학기술학회마을   DOI   ScienceOn
8 Kim H.K., Kang E.J., Ko B.S., Effect of the various aqua-aqupunctures of Hwanggum on proliferation of preadipocyte 3T3-L1 cells. K.O.M.S. 19(1):358-367, 1998.   과학기술학회마을
9 Rhee I.J., Kim E.J., Jeong S.W., Yang J.H., Lee I.S., Effects of Liriopis Tuber Extracts on Lipid Metabolism in Rats Fed High Cholesterol Diet. Kor. J. Pharmacogn. 34(1):65-69, 2003.   과학기술학회마을
10 Hotamisligil, G.S., Murray, D.L., Choy, L.N., Spiegelman, B.M. Tumor necrosis factor alpha inhibits signaling from the insulin receptor. Proc Natl Acad Sci. 91(11):4854-4858, 1994.   DOI   ScienceOn
11 Karastergiou, K., Mohamed-Ali, V. The autocrine and paracrine roles of adipokines. Mol Cell Endocrinol. 318(1-2):69-78, 2010.   DOI   ScienceOn
12 Lee, S.J., Kim, W.I., Kang, K.H. Inhibitory Effects of Bojungchiseub-tang on Adipocyte Differentiation and Adipogenesis in 3T3-L1 Preadipocytes. Korean J Oriental Physiology & Pathology. 27(4):343-349, 2013.
13 Maury, E., Brichard, S.M. Adipokine dysregulation, adipose tissue inflammation and metabolic syndrome. Mol Cell Endocrinol. 314(1):1-16, 2010.   DOI   ScienceOn
14 Kim, D., Lee, K. Study on Results of Obesity Management Center's Treatments. The Journal of Oriental Obstetrics and Gynecology. 14(1):319-327, 2001.
15 Kim, S., Jin, S.H., Kim, T.H. A Study on etiological factors, symptoms of a disease, and treatments of obesity based on Dong-Yi Bo Gam. J Korean Med. 19(2):125-136, 1998.   과학기술학회마을
16 Jin S.T. Seoksilbirok. Seoul, Haenglimseowon, p 76, 1982.
17 Lee, H.-C., Choi, Y.-M., Sim, W.-J., Kim, K.-S., Shin, S.-U., Choi, S.-M., Kang, B.-K. The Study of the Relationship with Abdominal Visceral Fat in Obese Patients Identified as Phlegm Type. J Korean Med Obes Res. 6: 51-59, 2006.   과학기술학회마을
18 Lee, H., Kim, M., Shik Shin, S., Yoon, M. Ginseng treatment reverses obesity and related disorders by inhibiting angiogenesis in female db/db mice. J Ethnopharmacol. 155(2):1342-1352, 2014.   DOI
19 Heo J. Daeyeok Dong-Yi Bo Gam. Seoul, Beopinmunhwasa, pp 1304-1305, 1999.
20 Kang, D.G., Kim, J.G., Kim, B.H., Cho, D.K., Sohn, E.J., Ryu, D.G., Lee, H.S. Effects of Bojungchiseup-tang on Renal Expression of Water Channels, Na, K-ATPase and Nitric Oxide Synthase in Rats. Korean journal of oriental physiology & pathology. 16(1):72-77, 2002.   과학기술학회마을
21 Kim, J.G., Kim, Y.G., Ryoo, J.H., Lee, H.S. Effects of Bojungchiseuptang on the Renal Function in Rats. Korean journal of oriental physiology & pathology. 15(2):296-299, 2001.   과학기술학회마을
22 Oh, J.K., Lee, M.J. The Effects of BoJungChiSeub-Tang Administration on Body Composition, Blood Biochemistrical Factors and Hormonal Changes during 2 weeks Weight Reduction in TaeKwonDo Athletes. J Korean Med. 19(2):391-400, 1998.   과학기술학회마을
23 Antuna-Puente, B., Feve, B., Fellahi, S., Bastard, J.-P. Adipokines: The missing link between insulin resistance and obesity. Diabetes Metab. 34(1):2-11, 2008.   DOI   ScienceOn
24 Murakami, M. Lipid Mediators in Life Science. Exp Anim. 60(1):7-20, 2011.   DOI
25 Tateya, S., Kim, F., Tamori, Y. Recent advances in obesity-induced inflammation and insulin resistance. Diabetes. 4: 93, 2013.
26 Galic, S., Oakhill, J.S., Steinberg, G.R. Adipose tissue as an endocrine organ. Mol Cell Endocrinol. 316(2):129-139, 2010.
27 Kapur, S., Marcotte, B., Marette, A. Mechanism of adipose tissue iNOS induction in endotoxemia. Am J Physiol - Endocrinol Metab. 276(4):E635-641, 1999.
28 Kamei, N., Tobe, K., Suzuki, R., Ohsugi, M., Watanabe, T., Kubota, N., Ohtsuka-Kowatari, N., Kumagai, K., Sakamoto, K., Kobayashi, M., Yamauchi, T., Ueki, K., Oishi, Y., Nishimura, S., Manabe, I., Hashimoto, H., Ohnishi, Y., Ogata, H., Tokuyama, K., Tsunoda, M., Ide, T., Murakami, K., Nagai, R., Kadowaki, T. Overexpression of Monocyte Chemoattractant Protein-1 in Adipose Tissues Causes Macrophage Recruitment and Insulin Resistance. J Biol Chem. 281(36):26602-26614, 2006.   DOI   ScienceOn
29 Wortman, P., Miyazaki, Y., Kalupahana, N.S., Kim, S., Hansen-Petrik, M., Saxton, A.M., Claycombe, K.J., Voy, B.H., Whelan, J., Moustaid-Moussa, N. n3 and n6 polyunsaturated fatty acids differentially modulate prostaglandin E secretion but not markers of lipogenesis in adipocytes. Nutr Metab. 6: 5, 2009.   DOI
30 Coleman, J.W. Nitric oxide in immunity and inflammation. Int Immunopharmacol. 1(8):1397-1406, 2001.   DOI   ScienceOn
31 Gil, A., Maria Aguilera, C., Gil-Campos, M., Canete, R. Altered signalling and gene expression associated with the immune system and the inflammatory response in obesity. Br J Nutr. 98(Supplement S1):S121-126, 2007.
32 Fujishiro, M., Gotoh, Y., Katagiri, H., Sakoda, H., Ogihara, T., Anai, M., Onishi, Y., Ono, H., Abe, M., Shojima, N., Fukushima, Y., Kikuchi, M., Oka, Y., Asano, T. Three Mitogen-Activated Protein Kinases Inhibit Insulin Signaling by Different Mechanisms in 3T3-L1 Adipocytes. Mol Endocrinol. 17(3):487-497, 2003.   DOI
33 Wada, T., Hoshino, M., Kimura, Y., Ojima, M., Nakano, T., Koya, D., Tsuneki, H., Sasaoka, T. Both type I and II IFN induce insulin resistance by inducing different isoforms of SOCS expression in 3T3-L1 adipocytes. Am J Physiol - Endocrinol Metab. 300(6):E1112-1123, 2011.   DOI
34 Lobo, S.M. de V., Quinto, B.M.R., Oyama, L., Nakamichi, R., Ribeiro, A.B., Zanella, M.T., Dalboni, M.A., Batista, M.C. TNF-$\alpha$ modulates statin effects on secretion and expression of MCP-1, PAI-1 and adiponectin in 3T3-L1 differentiated adipocytes. Cytokine. 60(1):150-156, 2012.   DOI
35 Zhang, H.H., Halbleib, M., Ahmad, F., Manganiello, V.C., Greenberg, A.S. Tumor Necrosis Factor-$\alpha$ Stimulates Lipolysis in Differentiated Human Adipocytes Through Activation of Extracellular Signal-Related Kinase and Elevation of Intracellular cAMP. Diabetes. 51(10):2929-2935, 2002.   DOI   ScienceOn
36 Nonogaki, K., Fuller, G.M., Fuentes, N.L., Moser, A.H., Staprans, I., Grunfeld, C., Feingold, K.R. Interleukin-6 stimulates hepatic triglyceride secretion in rats. Endocrinology. 136(5):2143-2149, 1995.
37 Waite, K.J., Floyd, Z.E., Arbour-Reily, P., Stephens, J.M. Interferon-$\gamma$-induced Regulation of Peroxisome Proliferator-activated Receptor $\gamma$ and STATs in Adipocytes. J Biol Chem. 276(10):7062-7068, 2001.   DOI
38 Schaloske, R.H., Dennis, E.A. The phospholipase A2 superfamily and its group numbering system. Biochim Biophys Acta. 1761(11):1246-1259, 2006.   DOI   ScienceOn
39 Kim C.K. (The)Effects of the rhizome of Atractylodes macrocephala Koidzumi on 3T3-L1 adipogenesis and on an obesity animal model. Kyung Hee University Doctorate thesis. 2009.