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Comparative Study of Characterizing Components and Biological Activities of Bangpungtongseong-san Formulation  

Kim, Jung Ok (Korean Traditional Medicine Agency, Korea Promotion Institute for Traditional Medicine Industry)
Choi, Hye Min (Korean Traditional Medicine Agency, Korea Promotion Institute for Traditional Medicine Industry)
Lee, Hee Hyun (Korean Traditional Medicine Agency, Korea Promotion Institute for Traditional Medicine Industry)
Moon, Sung Ok (Korean Traditional Medicine Agency, Korea Promotion Institute for Traditional Medicine Industry)
Kim, Jong Bum (Korean Traditional Medicine Agency, Korea Promotion Institute for Traditional Medicine Industry)
Lee, Hwa Dong (Korean Traditional Medicine Agency, Korea Promotion Institute for Traditional Medicine Industry)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.28, no.3, 2014 , pp. 322-329 More about this Journal
Abstract
The purpose of this study was to investigate the quality of two different commercial Bangpungtongseong-san (BTS) extract granules (BTS-2 and BTS-3) by comparing with BTS decoction (BTS-1). The contents of characterizing components and biological activities of two different commercial BTS extract granules were compared with those of the BTS decoction. The contents of characterizing components were analyzed with HPLC. The antioxidative effects were determined by measuring 2,2-diphenyl-1-picrylhygrazyl (DPPH) radical scavenging and superoxide dismutase (SOD)-like activity. Also, we compared the effects on lipid accumulation and reactive oxygen species (ROS) production during differentiation of 3T3-L1 preadipocytes. The contents of five components except liquiritin and sennoside A were higher in BTS-1. The DPPH radical scavenging and SOD-like activity were higher in BTS-1. BTS-1 significantly inhibited lipid accumulation during differentiation of 3T3-L1 preadipocytes and showed stronger effects than BTS-2, BTS-3. In addition BTS-1 showed stronger inhibition effects on ROS production during differentiation of 3T3-L1 preadipocytes than BTS-2, BTS-3. These results indicate that BTS decoction has strong biological activities than commercial BTS extract granules. It is also consistent with the contents of characterizing components.
Keywords
Bangpungtongseong-san; characterizing component; antioxidant; 3T3-L1 preadipocyte;
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