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Anti-cancer Effects of Oldenlandia diffusa, Cremastra appendiculata and Fritillaria thunbergii on MCF-7 Cells  

Jin, Myung Ho (Department of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University)
Hong, Sang Hoon (Department of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University)
Park, Cheol (Department of Molecular Biology, Dong-Eui University)
Choi, Yung Hyun (Department of Biochemistry, College of Korean Medicine, Dong-Eui University)
Park, Sang Eun (Department of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.28, no.3, 2014 , pp. 310-316 More about this Journal
Abstract
Oldenlandia diffusa, Cremastra appendiculata and Fritillaria thunbergii are widely distributed in the Korea, China and Japan, and has been used in traditional medicine for various diseases, such as pharyngolaryngitis, tonsillitis, goiter and stomach ulcer. However, the anti-cancer activities in human breast cancer have not been clearly elucidated yet. In this study, it was compared the in vitro cytotoxic effects of single and complex treatment of O. diffusa, C. appendiculata and F. thunbergii. We treat human breast cancer MCF-7 cells with O. diffusa, C. appendiculata and F. thunbergii. And we evaluated viability, growth inhibition, morphological changes, apoptotic bodies formation, measurement of the cell cycle and formation of DNA fragmentation of these cells. It was found that single treatment of O. diffusa could inhibit the cell proliferation in human breast cancer MCF-7 cells. However, complex treatment of O. diffusa, C. appendiculata and F. thunbergii is weakly or not affect the cell proliferation of MCF-7 cells. And anti-proliferative effects of O. diffusa in MCF-7 cells was associated with G1 arrest of cell cycle. These findings suggest that O. diffusa may be a potential chemotherapeutic agent for the control of human breast cancer cells and further studies will be needed to identify the molecular mechanisms.
Keywords
O. diffusa; C. appendiculata; F. thunbergii; human breast cancer cells; cell cycle arrest; anti-cancer;
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1 이효정, 이은옥, 이연희, 김관현, 이재호, 백남인, 등. 국내산 白花蛇舌草전초와 뿌리의 항암효과. 생약학회지 35(2):110-115, 2004.
2 김성훈. 白花蛇舌草로부터 分離한 ursolic acid의 自然殺害效果와 抗轉移作用. 大田大學校韓醫學硏究所論文集 5(2):523-533, 1997.
3 김성훈, 송규용, 유시용. 白花蛇舌草헥산分劃과 多糖體가 抗癌및 抗轉移活性에 미치는 影響(Ursolic acid와 Asperuloside 병용투여시 항암 및 항전이 효과에 관한 연구). 대한동의병리학회지 13(1):65-75, 1999.
4 노훈정, 문구, 문석재, 원진희, 문영호, 박래길. 白花蛇舌草메 탄올 抽出物의 抗腫瘍效果및 抗癌機轉에 關한 硏究. 대한한방종양학회지 6(1):81-97, 2000.
5 김경순, 예성철, 유병철, 조종관, 이연월, 유화승. 鬼箭羽, 白花蛇舌草, 瓦松추출물을 처치한 난소암과 자궁경부암 세포에서의 단백질 발현 변화. 대한한방내과학회지 32(1):33-42, 2011.
6 왕덕중, 강희, 심범상, 김성훈, 최승훈, 안규석. 加味慈桃丸構成藥物의 血管新生抑制效果에 관한 硏究. 대한암한의학회지 11(1):75-93, 2006.
7 이동은, 이소영, 김정선, 조종관, 유화승, 최선주. 抗癌丹및 그 주요 성분의 Calu6와 MCF-7 사람 암세포주에 대한 항암효과. 대한한의학회지 30(5):50-60, 2009.
8 한상순, 오중한, 곽민아, 변준석, 서정철, 한상원. 烏貝散의 항산화효과, 세포활성 및 cDNA microarray chip 유전자 발현에 미치는 영향. 대한본초학회지 18(2):59-70, 2003.
9 Ferlay, J., Shin, H.R., Bray, F., Forman, D., Mathers, C., Parkin, D.M. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer v 127: 2893-2917, 2010.   DOI   ScienceOn
10 Jalalian, A., Mashohor, S.B., Mahmud, H.R., Saripan, M.I,, Ramli, A.R., Karasfi, B. Computer-aided detection/diagnosis of breast cancer in mammography and ultrasound: a review. Clin Imaging 37: 420-426, 2013.   DOI   ScienceOn
11 Kalles, V., Zografos, G.C., Provatopoulou, X., Koulocheri, D., Gounaris, A. The current status of positron emission mammography in breast cancer diagnosis. Breast Cancer 20: 123-130, 2013.   DOI   ScienceOn
12 Gotzsche, P.C., Nielsen, M. Screening for breast cancer with mammography. Cochrane Database Syst Rev 1: 1877, 2011.
13 Meiyanto, E., Hermawan, A., Anindyajati. Natural products for cancer-targeted therapy: citrus flavonoids as potent chemopreventive agents. Asian Pac J Cancer Prev 13: 427-436, 2012.   DOI   ScienceOn
14 Li, Y., Wicha, M.S., Schwartz, S.J., Sun, D. Implications of cancer stem cell theory for cancer chemoprevention by natural dietary compounds. J Nutr Biochem 22: 799-806, 2011.   DOI   ScienceOn
15 Yang, H., Dou, Q.P. Targeting apoptosis pathway with natural terpenoids: implications for treatment of breast and prostate cancer. Curr Drug Targets 11: 733-744, 2010.   DOI   ScienceOn
16 Kousidou, O.Ch, Tzanakakis, G.N., Karamanos, N.K. Effects of the natural isoflavonoid genistein on growth, signaling pathways and gene expression of matrix macromolecules by breast cancer cells. Mini Rev Med Chem 6: 331-337, 2006.   DOI   ScienceOn
17 Molnár, J., Gyémánt, N., Tanaka, M., Hohmann, J., Bergmann-Leitner, E., Molnár, P., et al. Inhibition of multidrug resistance of cancer cells by natural diterpenes, triterpenes and carotenoids. Curr Pharm Des 12: 287-311, 2006.   DOI   ScienceOn
18 DeSantis, C., Naishadham, D., Jemal, A. Cancer statistics for African Americans. CA Cancer J Clin 63: 151-166, 2013.   DOI   ScienceOn
19 Jung, K.W., Won, Y.J., Kong, H.J., Oh, C.M., Seo, H.G., Lee, J.S. Cancer statistics in Korea: incidence, mortality, survival and prevalence in 2010. Cancer Res Treat 45: 1-14, 2013.   DOI   ScienceOn
20 Scherer, L.D., Ubel, P.A., McClure, J., Greene, S.M., Alford, S.H., Holtzman, L., et al.. Belief in numbers: When and why women disbelieve tailored breast cancer risk statistics. Patient Educ Couns 92: 253-259, 2013.   DOI   ScienceOn
21 Siegel, R., Naishadham, D., Jemal, A. Cancer statistics. CA Cancer J Clin 63: 11-30, 2013.   DOI   ScienceOn
22 Jemal, A., Siegel, R., Ward, E., Hao, Y., Xu, J., Thun, M.J. Cancer statistics. CA Cancer J Clin 59: 225-249, 2009.   DOI   ScienceOn
23 Siegel, R., DeSantis, C., Virgo, K., Stein, K., Mariotto, A., Smith, T., et al. Cancer treatment and survivorship statistics. CA Cancer J Clin 62: 220-241, 2012.   DOI
24 Ibrahim, T., Mercatali, L., Amadori, D. A new emergency in oncology: Bone metastases in breast cancer patients. Oncol Lett 6: 306-310, 2013.   DOI
25 Ferlay, J., Shin, H.R., Bray, F., Forman, D., Mathers, C., Parkin, D.M. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer 127: 2893-2917, 2010.   DOI   ScienceOn
26 薛己. 校注婦人良方大全. 文光圖書有限公司, pp 71-75.
27 Jun Ho Park, Sung Min Ju, Kun Jung Kim, Byung Hoon Jeon, Jung Mi Oh, Chae Ho Lee, et al. In vitro Growth Inhibition and Apoptotic Effects of Hang-baek-Tang on HL-60 Cells. Korean J Oriental Physiology & Pathology 19(6):1636-1639, 2005.
28 황기명. 人間白血病細胞株에서 芷貝散의 作用에 關한 硏究. 대한한의학 방제학회지 10(1):143-155, 2002.
29 金廷珍, 李京燮, 宋炳基. 乳房腫塊에 關한 文獻的考察. 대한한방부인과학회지 11(2):29-43, 1998.
30 許浚. 東醫寶鑑. 경남, 동의보감출판사, 683, 2006.