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Identification of the Plant Part of Gleditsia sinensis that Activates Nrf2, an Anti-oxidative Transcription Factor  

Choi, Jiyeon (Division of Applied Medicine, School of Korean Medicine, Pusan National University)
Kim, Kyun Ha (Division of Applied Medicine, School of Korean Medicine, Pusan National University)
Choi, Jun Yong (Department of Oriental Internal Medicine, Korean Medicine Hospital, Pusan National University)
Han, Chang Woo (Department of Oriental Internal Medicine, Korean Medicine Hospital, Pusan National University)
Ha, Ki Tae (Division of Applied Medicine, School of Korean Medicine, Pusan National University)
Jeong, Han-Sol (Division of Applied Medicine, School of Korean Medicine, Pusan National University)
Joo, Myungsoo (Division of Applied Medicine, School of Korean Medicine, Pusan National University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.28, no.3, 2014 , pp. 303-309 More about this Journal
Abstract
The fruit of Gleditsia sinensis has been extensively used as a key ingredient of an herbal remedy for the treatment of various inflammatory diseases in traditional Korean Medicine. However, the reason of using the fruit of G. sinensis for the remedy is unclear. Since Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is a key anti-inflammatory transcription factor, which is activated by the fruit of G. sinesis, we examined whether other plant parts of G. sinensis are also capable of suppressing inflammatory responses by activating Nrf2. Water extracts of various parts of G. sinensis were prepared and tested for Nrf2 activation by reporter assay and western blot analysis. Our results show that the hull of G. sinensis is the most potent in activating Nrf2. Sequential organic solvent extraction of the hull show that all the fractions had a higher potency in activating Nrf2 than the water extract, albeit differential degrees. The hull originated from Korea in general activated Nrf2 strongly compared to that of China. Chloroform fraction of the hull was further examined, showing that the fraction induced nuclear localization of Nrf2, indicative of activated Nrf2, and Nrf2-dependent gene expression including NAD(P)H dehydrogenase quinone 1 (NQO-1), glutamate-cysteine ligase catalytic subunit (GCLC), and heme oxygenase - 1 (HO-1). Therefore, our results show that, among other plant parts examined in this study, the hull of G. sinensis is the most potent, providing the experimental basis for the use of the hull of G. sinensis as an active ingredient for an anti-inflammatory remedy.
Keywords
Nrf2; Anti-oxidant response element; Gleditsia sinensis;
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