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Effects of Hoesaeng-san Ethanol Extract on the Human Mast cell-mediated Inflammatory Responses  

Park, Jee Hea (Department of Third Medicine, Professional Graduate School of Korean Medicine, Wonkwang University)
Kwon, Dong Yeol (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Lee, Su Kyung (Department of Oriental Rehabilitation Medicine, College of Korean Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.28, no.1, 2014 , pp. 45-52 More about this Journal
Abstract
Hoesaeng-san is known to be effective for treating diarrhea and vomiting. However the therapeutic mechanism of Hoesaeng-san is still not well understood. The aim of the present study was to demonstrate the effects of Hoesaeng-san ethanol extract (HSSEE) on the expression of pro-inflammatory cytokines, as well as to elucidate its mechanism of action in the human mast cell line (HMC-1). Mast Cell were stimulated with phorbol 12-myristate 13-acetate (PMA) plus A23187 in the presence or absence of HSSEE. To study the possible effects of HSSEE, ELISA, RT-PCR, Western blot analysis were used in this study. HSSEE significantly inhibited the PMA plus A23187-induction of inflammatory cytokines such as tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-6 and IL-8. In activated HMC-1 cells, phosphorylation of extra-signal response kinase (ERK) 1/2 and c-jun n-terminal kinase (JNK)1/2 decreased after treatment with HSSEE. Moreover HSSEE inhibited PMA plus A23187-induced nuclear factor (NF)-${\kappa}B$ activation and $I{\kappa}B$ degradation. HSSEE suppressed the expression of TNF-${\alpha}$, IL-6, IL-8 through a decrease in the ERK 1/2 and JNK 1/2, as well as activation of NF-${\kappa}B$. These results indicated that HSSEE exerted a regulatory effect on inflammatory reactions mediated by mast cells.
Keywords
Hoesaeng-san; mast cell; inflammatory reactions;
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