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Effect of Lespedezea Cuneata on the Contraction of Rabbit Common Carotid Artery and Corpus Cavernosum  

Park, Sun Young (Department of Physiology, College of Korean Medicine, Semyung University)
Kim, Ho Hyun (Department of Physiology, College of Korean Medicine, Semyung University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.27, no.6, 2013 , pp. 809-817 More about this Journal
Abstract
The aim of this study was to evaluate the mechanism of vasodilation of Lespedezea cuneata(LC) in rabbit common carotid artery and cavernosal smooth muscle. LC relaxed arterial strips precontracted with norepinephrine and cavernosal strips precontracted with phenylephrine. The arterial relaxation effects of LC was endothelium-dependent. $N{\omega}$-nitro-L-arginine(L-NNA), NOS inhibitor, methylene blue(MB), cGMP inhibitor, indomethacin(IM), cyclo-oxygenase inhibitor and tetraethylammonium chloride(TEA), KCa-channel blocker attenuate the relaxation responses of LC in arterial strips. In $Ca^{2+}$-free krebs-ringer solution, pretreatment of LC extract significantly reduced the contraction induced by addition $Ca^{2+}$. L-NNA reduced LC extract-induced relaxation in cavernosal strips, but IM, TEA and MB didn't affect LC extract-induced relaxation. When LC extract was applicated on human umbilical vein endothelial cell, the nitric oxide concentration was increased. We conclude that in rabbit common carotid artery, LC may suppress influx of extra-cellular $Ca^{2+}$ through the release of endothelium derived relaxing factor including nitric oxide, prostacyclin, endothelium derived hyperpolarizing factor. And LC exerts a relaxing effect on corpus cavernosum through activating the NO.
Keywords
Lespedezea cuneata; nitric oxide; corpus cavernosum; arterial relaxation;
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1 Traish, A.M., Moreland, R.B., Huang, Y.H., Goldstein, I. Expression of functional alpha2-adrenergic receptor subtypes in human copus cavernosum and in cultured trabecular smooth muscle cells. Recept Signal Transduct 7: 55-67, 1997.
2 Lee, S.W., Wang, H.Z., Zhao, W., Ney, P., Brink, P.R., Christ, G.J. Prostaglandin, E.1. activates the large-conductance KCa channel in human corporal smooth muscle cells. International Journal of Impotence Research 11: 189-199, 1999.   DOI   ScienceOn
3 Wang, H.Z., Lee, S.W., Chris,t G.J. Comparative studies of the maxi-K(K(Ca)) channel in freshly isolated myocytes of human and rat corpora. International Journal of Impotence Research 12: 9-18, 2000.   DOI
4 Lee, S.W., Wang, H.Z., Christ, G.J. Characterization of ATP-sensitive potassium channels in human corporal smooth muscle cells. International Journal of Impotence Research 11: 179-188, 1999.   DOI
5 Palmer, R.M.J., Ashton, D.S., Moncada, S. Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature 333: 664-666, 1989.
6 Guyton, A.C. Medical Physiology. W. B. Saunders Company, 20: 230-243, 1986.
7 Vanhoutte, P.M. Endothelial control of vasomotor function: from health to coronary disease. Circulation journal 67(7):572-575, 2003.   DOI   ScienceOn
8 Segarra, G., Medina, P., Revert, F., Masia, S., Vila, J.M., Such, L., Aldasoro, M. Modulation of adrenergic contraction of dog pulmonary arteries by nitric oxide and prostacyclin. General Pharmacol. 32: 583-589, 1999.   DOI   ScienceOn
9 Hecker, M. Endothelium derived hyperpolarizing factor-Fact or Fiction? News Physiol. Sci. 15: 1-5, 2000.
10 Fleming, I. Cytochrome P540 2C is an EDHF Synthase in Coronary Arteries. Trends Cardiovasc. Med. 10: 166-170, 2000.   DOI   ScienceOn
11 Johns, R.A. Endothelium-derived relaxing factor : Basic review and clinical implications. J. Cardiothorac. Vasc. Anesth. 5(1):69-79, 1991.   DOI   ScienceOn
12 Hao, H., Fiscus, R.R., Wang, X. $N{\omega}$-nitro-L-arginine inhibits vasodilations and elevations of both cyclic AMP and cyclic GMP levels in rat aorta induced by calcitonin gene-related peptide(CGRP). Neuropeptides 26(2):123-131, 1994.   DOI   ScienceOn
13 Ignarro, L.J., Harbison, R.G., Wood, K.S., Kadowitz, P.J. Activation of purified soluble guanylate cyclase by endothelium-derived relaxing factor from intrapulmonary artery and vein : Stimulation by acetylcholine, bradyklinin and arachidonic acid. The J. of Pharmacol. and Experimental therapeutics 237(3):893-900, 1986.
14 Mayer, B., Brunner, F., Schmid, K. Inhibition of nitric oxide synthesis by methylene blue. Biochemical Pharmacology 45(2):367-374, 1993.   DOI   ScienceOn
15 Deth, R., Breemen, C. Relative contributions of Ca++ influx and cellular Ca++ release during drug induced activation of the rabbit aorta. Pflugers Arch. 348(1):13-22, 1974.   DOI   ScienceOn
16 Abran, D., Hardy, P., Varma, D.R. Mechanisms of the biphasic effects of peroxides on the retinal vasculature of newborn and adult pigs. Experimental eye research 61(3):285-292, 1995.   DOI   ScienceOn
17 Fabiato, A., Fabiato, F. Calcium-induced release of calcium from the sarcoplasmic reticulum of skinned cells from adult human, dog, cat, rabbit, rat, and frog hearts and from fetal and new-born rat ventricles. Annuals New York Academy of Sciences pp 491-521, 1978.
18 De May, J.G., Vanhoutte, P.M. Rolo of the intima in cholinergic and purinergic relaxation of isolated canin femoral arteries. Journal of Physiology 316: 347-355, 1981.   DOI
19 Bruce, M. Koeppen, Bruce A. Stanton. BERNE & LEVY 생리학. 서울, 이퍼블릭, pp 767-768, 2009.
20 Andersson, K.E., Wagner, G. Physiology of penile erection. Physiology Research 75: 191-236, 1995.
21 江蘇中醫學院. 中藥大辭典(上). 大邱, 大成出版社, p 1444, 1984.
22 배기환. 한국의 약용식물. 서울, 교학사, p 255, 2003.
23 김용한, 류수노. 비수리(Lespedeza cuneata G. Don) 지상부 메탄올 추출물의 항산화 활성. 한작지 53(S):121-123, 2008.
24 김현영, 고지연, 송석보, 김정인, 서혜인, 이재생, 곽도연, 정태욱, 김기영, 오인석, 정헌상, 우관식. 비수리(Lespedeza cuneata G. Don) 메탄올 추출물로부터 분획된 용매분획물의 항산화활성과 ${\alpha}$-Glucosidase 저해활성. 한국식품영양과학회지 41(11):1508-1514, 2012.
25 Lee, H.J., Lim, G.N., Park, M.A., Park, S.N. Antibacterial and Antioxidative Activity of Lespedeza cuneata G. Don Extracts. Korean J. Microbiol. Biotechnol. 39(1):63-70, 2005.
26 최정식, 조충식, 김철중. 夜關門의 포도당 독성에 대한 세포보호 효과. 대한한의학회지 31(4):79-100, 2010.
27 김혜정, 김길수, 김대익. 야관문 에탄올 추출물의 자외선 조사에 의한 피부 광노화 억제 효과. 한국식품영양과학회지 41(11):1540-1545, 2012.
28 이준경. 야관문의 NO/cGMP계를 경유한 혈관이완효과. 원광대학교 대학원, 석사학위논문, 2006.
29 정영호, 임치환. 야관문 (Lespedezea cuneata G. Don) 추출물이 토끼 음경해면체 평활근에 미치는 생리활성. 농업과학연구 32(1):63-70, 2005.
30 Hedlund, H., Andersson, K.E. Contraction and relaxation induces by some prostanoids in isolated human penile erectile tissue and cavernous artery. Journal of Urology 15(1):9-15, 1988.
31 Saenz de Tejada, I., Goldstein, I., Azadoi, K., Krane, R.I., Cohen, R.H. Impaired neurogenic and endothelium mediated relaxation of penile smooth muscle from diabetic men with impotince. The New England Journal of Medicine 320: 1025-1030, 1989.   DOI   ScienceOn
32 Stamler, J.S., Singel, D.J., Loscalzo J. Biochemistry of nitric oxide and its redox activated forms. Science 258:1898-1902, 1992.   DOI