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Single Oral Dose Toxicity Study of Water Extracts of Polygalae Radix in ICR Mice  

Kang, Byung Hoon (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Ku, Sae Kwang (Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University)
Seo, Bu Il (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Roh, Seong Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Park, Soo Jin (Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University)
Park, Ji Ha (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.27, no.4, 2013 , pp. 453-459 More about this Journal
Abstract
The objective of this study was to evaluate the single oral dose toxicity of Polygalae Radix (PR) in male and female mice. PR extract (yield = 18.6%) was administered to ICR mice as an oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) Guidelines (2009-116, 2009). Animals were monitored for the mortality and the changes in body weight, clinical signs and gross observation during 14 days after dosing. Upon necropsy, organ weight and histopathology of 14 principal organs were examined. It was observed that there were no mortalities, clinical signs, changes on the body and organ weights, gross and histopathological observations against 14 principal organs related to PR extract up to 2,000 mg/kg. Therefore, 50% lethal dose ($LD_{50}$) and approximate LD of PR aqueous extracts after single oral treatment in female and male mice were considered over 2000 mg/kg the limited dosages recommended by KFDA Guidelines, respectively.
Keywords
Polygalae Radix; Single oral dose toxicity; Mouse; Histopathology;
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1 Lee, B.J., Ahn, B.W., Kang, H.G., Kim, Y.B. Four-week repeated-dose toxicity study on Pinellia Extract. Korean J Lab Anim Sci 19: 127-141, 2003.
2 Roh, S.S., Ku, S.K. Mouse single oral dose toxicity study of DHU001, a polyherbal formula. Toxicol Res 26: 53-59,2010.   DOI
3 Cheong, M.H., Lee, S.R., Yoo, H.S., Jeong, J.W., Kim, G.Y., Kim, W.J., Jung, I.C., Choi, Y.H. Anti-inflammatory effects of Polygala tenuifolia root through inhibition of NF-B activation in lipopolysaccharide-induced BV2 microglial cells. J Ethnopharmacol 137: 1402-1408, 2011.   DOI   ScienceOn
4 Choi, J.G., Kim, H.G., Kim, M.C., Yang, W.M., Huh, Y., Kim, S.Y., Oh, M.S. Polygalae radix inhibits toxin-induced neuronal death in the Parkinson's disease models. J Ethnopharmacol 134: 414-421, 2011.   DOI   ScienceOn
5 Liang, Z., Shi, F., Wang, Y., Lu, L., Zhang, Z., Wang, X., Wang, X. Neuroprotective effects of tenuigenin in a SH-SY5Y cell model with 6-OHDA-induced injury. Neurosci Lett 497: 104-109, 2011.   DOI   ScienceOn
6 Lee, S.B., Seong, N.S., Lee, Y.J. A study on the protective effects of Polygalae Radix on neurotoxicity induced by N-methyl-D-aspartic acid (NMDA). Kor. J. Herbology 20(2):115-125, 2005
7 Hu, Y., Liu, M., Liu, P., Guo, D.H., Wei, R.B., Rahman, K. Possible mechanism of the antidepressant effect of 3,6'-disinapoyl sucrose from Polygala tenuifolia Willd. J Pharm Pharmacol 63: 869-874, 2011.   DOI   ScienceOn
8 Hu, Y., Liu, P., Guo, D.H., Rahman, K., Wang, D.X., Xie, T.T. Antidepressant effects of the extract YZ-50 from Polygala tenuifolia in chronic mild stress treated rats and its possible mechanisms. Pharm Biol 48: 794-800, 2010.   DOI   ScienceOn
9 Ikeya, Y., Takeda, S., Tunakawa, M., Karakida, H., Toda, K., Yamaguchi, T., Aburada, M. Cognitive improving and cerebral protective effects of acylated oligosaccharides in Polygala tenuifolia. Biol Pharm Bull 27: 1081-1085, 2004.   DOI   ScienceOn
10 Karakida, F., Ikeya, Y., Tsunakawa, M., Yamaguchi, T., Ikarashi, Y., Takeda, S., Aburada, M. Cerebral protective and cognition-improving effects of sinapic acid in rodents. Biol Pharm Bull 30: 514-519, 2007.   DOI   ScienceOn
11 Xu, S.P., Yang, Y.Y., Xue, D., Liu, J.X., Liu, X.M., Fan, T.P., le Pan, R., Li, P. Cognitive-enhancing effects of polygalasaponin hydrolysate in a(25-35)-induced amnesic mice. Evid Based Complement Alternat Med 2011: 839720, 2011.
12 Kawashima, K., Miyako, D., Ishino, Y., Makino, T., Saito, K., Kano, Y. Anti-stress effects of 3,4,5-trimethoxycinnamic acid, an active constituent of roots of Polygala tenuifolia (Onji). Biol Pharm Bull 27: 1317-1319, 2004.   DOI   ScienceOn
13 Hu, Y., Liao, H.B., Liu, P., Guo, D.H., Rahman, K. A bioactive compound from Polygala tenuifolia regulates efficiency of chronic stress on hypothalamic-pituitary-adrenal axis. Pharmazie 64: 605-608, 2009.
14 Yao, Y., Jia, M., Wu, J.G., Zhang, H., Sun, L.N., Chen, W.S., Rahman, K. Anxiolytic and sedative-hypnotic activities of polygalasaponins from Polygala tenuifolia in mice. Pharm Biol 48: 801-807, 2010.   DOI   ScienceOn
15 Lee, T.H. The research on the pharmacological concept of sedative effect of Polygalae Radix. Kor. J. Herbology 12(1):1-6, 1997.
16 Park, J.H., Kim, J.S., Jang, D.S., Lee, S.M. Effect of Polygala tenuifolia root extract on cerebral ischemia and reperfusion. Am J Chin Med 34: 115-123, 2006.   DOI   ScienceOn
17 Kim, K.H., Jeong, H.W., Lee, D.W., Kim, J.S., Kim, Y.G., Cho, S.I. Effects of Radix Polygalae extract on sociopsychological stress. Kor. J. Herbology 18(1):99-108, 2003.
18 Park, H.J., Lee, K., Heo, H., Lee, M., Kim, J.W., Whang, W.W., Kwon, Y.K., Kwon, H. Effects of Polygala tenuifolia root extract on proliferation of neural stem cells in the hippocampal CA1 region. Phytother Res 22: 1324-1329, 2008.   DOI   ScienceOn
19 Spelman, K., Burns, J., Nichols, D., Winters, N., Ottersberg, S., Tenborg, M. Modulation of cytokine expression by traditional medicines: a review of herbal immunomodulators. Altern Med Rev 11: 128-150, 2006.
20 Zhu, Y.P. Chinese Materia Medica Chemistry. Pharmacology and Applications. The Netherlands, Harwood Academic Publishers, pp 516-519, 1998.
21 Park, H.K., Jeon, S.G., Kim, T.B., Kang. H.R., Chang, Y.S., Kim, Y.K., Cho, S.H., Min, K.U., Kim, Y.Y. Occupational asthma and rhinitis induced by a herbal medicine, Wonji (Polygala tenuifolia). J Korean Med Sci 20: 46-49, 2005.   DOI   ScienceOn
22 Korea Food and Drug Administration. Testing Guidelines for Safety Evaluation of Drugs (Notification No. 2009-116, issued by the Korea Food and Drug Administration on August 24, 2009). 2009.
23 Irwin, S. Comprehensive observational assessment: Ia. A systemic, quantitative procedure for assessing the behavioral and physiological state of the mouse. Psychopharmacology 13: 222-257, 1968.   DOI   ScienceOn
24 Dourish, C.T. Effects of drugs on spontaneous motor activity in Experimental psychopharmacology (Greenshaw, A.J. and Dourish, C.T., Eds.), Clifton, Humana Press.325-334, 1987.
25 Levene, A. Pathological factors influencing excision of tumours in the head and neck. Part I. Clin Otalary. 6: 145-151, 1981.   DOI
26 Ludbrook, J. Update: microcomputer statistics packages. A personal view. Clin Exp Pharmacol Physiol 24: 294-296, 1997.   DOI   ScienceOn
27 Yamaguchi, C., Fujita, S., Obara, T., Ueda, T. Effects of room temperature on reproduction, body weight and organ weights, food and water intakes, and hematology in mice. Exp Anim 32: 1-11, 1983.
28 Lee, W.H., Gam, C.O., Ku, S.K., Choi, S.H. Single oral dose toxicity test of platycodin D, a saponin from platycodin radix in mice. Toxicol Res 27: 217-224, 2011.   DOI   ScienceOn
29 Flecknell, P. Laboratory Animal Anesthesia. 2nd Ed., New York, Harcourt Brace & Company, 269, 1996.
30 Plata, E.J., Murphy, W.H. Growth and haematologic properties of the BALB/wm strain of inbred mice. Lab Anim Sci 22: 712-720, 1972.
31 Banks, W.J. Female reproductive system in Applied veterinary histology (Banks WJ, Ed). Baltimore, Williams & Wilkins. pp 506-526, 1986.
32 Pineda, M.H. Female reproductive system in Veterinary endocrinology and reproduction (McDonald LE, Pineda MH, Eds.). Philadelphia, Lea & Febiger, pp 303-354, 1989.