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Ethanol Extract of Dioscorea batatas Stimulates Procollagen Production and Reduces UVB-induced MMPs Activity in Skin  

Kim, Dae Sung (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University)
Jeon, Byoung Kook (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University)
Lim, Nan Young (Department of Beauty Design, College of Environmental Resources, Wonkwang University)
Mun, Yeun Ja (Department of Anatomy, College of Korean Medicine, Wonkwang University)
Lee, Young Eun (Department of Food and Nutrition, College of Environmental Resources, Wonkwang University)
Woo, Won Hong (Department of Anatomy, College of Korean Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.27, no.2, 2013 , pp. 183-188 More about this Journal
Abstract
Ultraviolet (UV) B irradiation induces the production of matrix metalloproteinases (MMPs), which are responsible for the degradation or synthesis inhibition of collagenous extracellular matrix in connective tissues, causing skin photoaging. In this study, we examined the inhibitory effect of MMP-1 expression of yam extract in tumor necrosis factor-alpha (TNF-${\alpha}$)-stimulated human dermal fibroblast neonatal (HDFn) cell and preventive effect of UVB-induced damage in hairless mice skin. The synthesis of procollagen and the release of MMP-1 in HDFn cells were measured by EIA kit and MMP-1 assay kit, respectively. UVB radiation was applied to the backs of the mice three times a week for 8 weeks. Mice were randomly divided into three groups, and were topical application with the Dioscorea batatas (DB, 6%) or vehicle. Reduction of TNF-${\alpha}$-induced procollagen synthesis was increased by DB (50 ug/ml), which was higher than positive control group (TGF-${\beta}$). Also, pre-treatment of HDFn cells with DB inhibited TNF-${\alpha}$-induced release of MMP-1. In vivo study, we found that preventive effect of DB against UV-induced epidermal thickness. DB suppressed the expression of MMP-3 and MMP-13 induced by UVB irradiation. Our results show that DB have preventive effect of UV-induced skin damage in hairless mice.
Keywords
MMPs; Dioscorea batatas; procollagen; UV; skin;
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1 Gross, B., Lapiere, C. Collagenolytic activity in amphibian tissue a tissue culture assay. Proc Natl Acad Sci USA 54: 1197-1204, 1962.
2 Steenport, M., Khan, K.M., Du, B., Barnhard, S.E., Dannenberg, A.J., Falcone, D.J. Matrix Metalloproteinase (MMP)-1 and MMP-3 Induce Macrophage MMP-9:Evidence for the Role of TNF-a and Cyclooxygenase-2. J Immunol 183(12):8119-8127, 2009.   DOI   ScienceOn
3 Kim, H.H., Lee, M.J., Lee, S.R., Kim, K.H., Cho, K.H., Eun, H.C., Chung, J.H. Augmentation of UV-induced skin wrinkling by infrared irradiation in hairless mice. Mech Ageing Dev. 126(11):1170-1177, 2005.   DOI   ScienceOn
4 Kang, S.W., Chung, J.H., Hammerberg, C., Fisher, G.J., Voorhes, J.J. Inflammatio and Extracellular Matrix Degradation Mediated by Activated ranscription Factors Nuclear Factor-kB and Activator Protein-1 in Inflammatory Acne Lesions in Vivo. Am J Pathol 166: 1691-1699, 2005.   DOI   ScienceOn
5 McCord, J.M., Fridovich, I. The utility of superoxide dismutase in studying free radical reactions. I., Radicals generated by the interaction of sulfite, dimethyl sulfoxide, and oxygen. J Biol Chem. 25: 6056-6063, 1963.
6 Kim, N.M., Koo, B.S., Lee, S.K., Hwang, E.I., So, S.H., Do J.H. Effect of Korean red ginseng on collagen biosynthesis and MMP-I activity in human dermal fibroblast. J Ginseng Res. 31(2):86-92, 2007.   DOI
7 Fisher, G.J., Wang, Z.Q., Datta, S.C., Varani, J., Kang, S., Voorhees, J.J. Pathophysiology of premature skin aging induced by ultraviolet light. N Engl J Med 337: 1419-1428, 1997.   DOI   ScienceOn
8 Kim, H.H., Cho, S., Lee, S., Kim, K.H., Cho, K.H., Eun, H.C., Chung, J.H. Photoprotective and anti-skinaging effects of eicosapentaenoic acid in human skin in vivo. J Lipid Res 47: 921-930, 2006.   DOI   ScienceOn
9 Lee, K.E., Mun, S.Y., Pyun, H.B., Kim, M.S., Hwang, J.K. Effects of Macelignan Isolated from Myristica fragrans (Nutmeg) on Expression of Matrix Metalloproteinase-1 and Type I Procollagen in UVB-Irradiated Human Skin Fibroblasts. Biol Pharm Bull 35(10):1669-1675, 2012.   DOI
10 Jean, L.B., Aging skin. Am J Med 8: S99-S103, 1995.
11 Laure, R.G., Fisher, J. UV-light-induced signal cascades and skin aging. Ageing Res Rev. 1: 705-720, 2002.   DOI   ScienceOn
12 Lee, Y.R., Noh, E.M., Han, J.H., Kim, J.M., Hwang, J.K., Hwang, B.M., Chung, E.Y., Kim, B.S., Lee, S.H., Lee, S.J., Kim, J.S. Brazilin inhibits UVB-induced MMP-1/3 expressions and secretions by suppressing the NF-${\kappa}B$ pathway in human dermal fibroblasts. Eur J Pharmacol 15;674(2-3):80-86, 2012.   DOI   ScienceOn
13 Oh, J.H., Kim, A., Park, J.M., Kim, S.H., Chung, A.S. Ultraviolet B-Induced Matrix Metalloproteinase-1 and -3 Secretions Are Mediated via PTEN/Akt Pathway in Human Dermal Fibroblasts. J Cell Physiol 209(3):775-785, 2006.   DOI   ScienceOn
14 Zhang, M., Zhou, Q., Liang. Q.Q., Li, C.G., Holz, J.D., Tang, D., Sheu, T.J., Li, T.F., Shi, Q., Wang, Y.J. IGF-1 regulation of type II collagen and MMP-13 expression in rat endplate chondrocytes via distinct signaling pathways. Osteoarthritis Cartilage 17(1):100-106, 2009.   DOI   ScienceOn
15 Mariani, T.J., Sandefur, S., Roby, J.D., Pierce, R.A. Collagenase-3 induction in rat lung fibroblasts requires the combined effects of tumor necrosis factor-alpha and 12-lipoxygenase metabolites: a model of macrophage-induced, fibroblast-driven extracellular matrix remodeling during inflammatory lung injury. Mol Biol Cell 9(6):1411-1424, 1998.   DOI   ScienceOn
16 김나미, 구본석, 이성계, 황의일, 소승호, 도재호. 홍삼성분이 섬유아세포의 콜라겐 생합성과 MMP-1 활성에 미치는 영향. 고려인삼학회 31(2):86-92, 2007.
17 Cho, S., Kim, H.H., Lee, M.J., Lee, S., Park, C.S., Nam, S.J., Han, J.J., Kim, J.W., Chung, J.H. Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivo. J Lipid Res 49(6):1235-1245, 2008.   DOI   ScienceOn
18 Chung, J.H., Eun, H.C. Angiogenesis in skin aging and photoaging. J Dermatol 34(9):593-600, 2007.   DOI   ScienceOn
19 Durai, P.C., Thappa, D.M., Kumari, R., Malathi, M. Aging in elderly: chronological versus photoaging. Indian J Dermatol 57(5):343-352, 2012.   DOI   ScienceOn
20 Fisher, G.J., Kang, S., Varani, J., Bata-Csorgo, Z., Wan, Y., Datta, S., Voorhees, J.J. Mechanisms of photoaging and chronological skin aging. Arch Dermatol 138(11):1462-1470, 2002.
21 Yang, B., Ji, C., Kang, J., Chen, W., Bi, Z., Wan, Y. Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts. Int J Mol Med 23(4):555-560, 2009.
22 Oh, J.H., Kim, A., Park, J.M., Kim, S.H., Chung, A.S. Ultraviolet B-induced matrix metalloproteinase-1 and -3 secretion are mediated via PTEN/Akt pathway in human dermal fibroblasts. J Cell Physiol 209(3):775-785, 2006.   DOI   ScienceOn
23 Soter, N.A. Acute effects of ultraviolet radiation on the skin. Semin Dermatol 9(1):11-15, 1990.
24 Lee, Y.R., Noh, E.M., Jeong, E.Y., Yun, S.K., Jeong, Y.J., Kim, J.H., Kwon, K.B., Kim, B.S., Lee, S.H., Park, C.S., Kim, J.S. Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-kappaB pathway in human dermal fibroblasts. Exp Mol Med 31;41(8):548-554, 2009.
25 정신교, 정용열, 정우식. 단마(Dioscorea aimadoimo)의 열풍 건조 시 갈변 억제 방안 연구. 한국응용생명화학회지 39(5):384-388, 1996.
26 김명화, 임숙자. 참마 분획물이 당뇨 유발 흰주의 혈당 및 에너지대사물농도에 미치는 영향. 한국영양학회지 31(7):1093-1099, 1998.
27 Kwon, C.S., Sohn, H.Y., Kim, S.H., Kim, J.H., Son, K.H., Lee, J.S., Lim, J.K., Kim, J.S. Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents. Biosci Biotechnol Biochem 67: 1451-1456, 2003.   DOI   ScienceOn
28 Kwon, E.G., Choe, E.M., Gu, S.J. Effects of mucilage from yam (Dioscorea btatas DECNE) on blood glucose and lipid composition in alloxan-induced diabetic mice. Korean J Food Sci Technol 33: 795-801, 2001.
29 Son, I.S., Kim, J.I., Sohn, H.Y., Son, K.H., Kim, J.S., Kwon, C.S. Antioxidative and hypolipidemic effects of diosgenin, a steroidal saponin of yam (Dioscorea spp), on hing-cholesterol fed rats. Biosci Biotechnol Biochem 71: 3063-3071, 2007.   DOI   ScienceOn
30 Kang, T.H., Choi, S.Z., Lee, T.H., Son, M.W., Kim, S.Y. Characteristics of antidiabetic effect of Dioscorea rhizoma (1) - Hypoglycemic effect. Korean J Food Nutr 21: 425-429, 2008.
31 Han, Y.N., Hahn, S.H., Lee, I.R. Purification of mucilages from Dioscorea batatas and D. Japonica and their content analysis. Korean J Pharmacogn 21: 274-283, 1990.
32 Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63, 1983.   DOI   ScienceOn
33 Fisher, G.J., Datta, S., Wang, Z., Li, X.Y., Quan, T., Chung, J.H., Kang, S., Voorhees, J.J. Fisher GJ, Datta S, Wang Z, Li XY, Quan T, Chung JH, Kang S, Voorhees JJ. J Clin Invest 106(5):663-670, 2000.   DOI   ScienceOn
34 Fagot, D., Asselineau, D., Bernerd, F. Matrix metalloproteinase-1 production observed after solar-stimulated radiation exposure is assumed by dermal fibroblasts but involves a paracrine activation through epidermal keratinocyte. Photochem Photobiol 79: 499-505, 2004.   DOI   ScienceOn
35 Brinckmann, J., Acil, Y., Wolff, H.H., Muller, P.K. Collagen synthesis in (sun-) aged human skin and in fibroblasts derived from sun-exposed and sin-protected body sites. J Photochem Photobiol B 27: 33-38, 1995.   DOI   ScienceOn