Browse > Article

Effect of Vigna angularis on Toll-like Receptor Activation and Pro-inflammatory Cytokine Production  

Kim, Mi-Hwa (Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology)
Jeoung, See-Hwa (Natural Bio R&D Center, Sun Bio Tech)
Lee, Seung-Woong (Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology)
Kim, Hyun-Kyu (Natural Bio R&D Center, Sun Bio Tech)
Park, Chan-Sun (Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology)
Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine & Research Center of Traditional Korean Medicine, Wonkwang University)
Oh, Hyun-Mee (Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology)
Rho, Mun-Chual (Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.26, no.4, 2012 , pp. 511-518 More about this Journal
Abstract
The mechanisms of Toll-like receptor (TLR) signaling have been the focus of extensive studies because TLRs are the target of therapeutic intervention on multiple diseases. In this study, we investigated the inhibitory potential of Vigna angularis (azuki bean) on the TLR signaling. The effect of Vigna angularis extract (JSD) on TLR activation was investigated by assessing NF-${\kappa}B$ and AP-1 inducible secreted embryonic alkaline phosphatase (SEAP) activity. JSD significantly inhibited SEAP activity induced by poly I:C (TLR3 ligand) and poly I (TLR7 ligand) in a dose-dependent manner at concentration below 100 ${\mu}g/ml$ with no sign of cytotoxicity. Pretreatment of JSD markedly suppressed mRNA expressions of pro-inflammatory cytokines and adhesive molecules such as TNF-${\alpha}$, IL-6, RANTES, MCP-1 and ICAM-1 induced by TLR ligands. It also diminished the phosphorylation of $I{\kappa}B$ kinase and $I{\kappa}B$, and followed by $I{\kappa}B$-mediated nuclear translocation of p50, p65, and phosphorylation of p38, JNK, and IRF signaling pathway. In conclusion, our results suggest that Vigna angularis has inhibitory activity on TLR-3 and -7 signaling and it can be further developed as a remedy in curing TLR-related multiple diseases.
Keywords
Toll-like receptor; Vigna angularis; NF-${\kappa}B$; TNF-${\alpha}$; IL-6;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Akira, S., Takeda, K., Kaisho, T. Toll-like receptors: critical proteins linking innate and acquired immunity. Nature immunology. 2(8):675-680, 2001.   DOI   ScienceOn
2 Akira, S., Uematsu, S., Takeuchi, O. Pathogen recognition and innate immunity. Cell. 124(4):783-801, 2006.   DOI   ScienceOn
3 Hemmi, H., Takeuchi, O., Kawai, T., Kaisho, T., Sato, S., Sanjo, H., Matsumoto, M., Hoshino, K., Wagner, H., Takeda, K., Akira, S. A Toll-like receptor recognizes bacterial DNA. Nature. 408(7):740-745, 2000.
4 Hayashi, F., Smith, K.D., Ozinsky, A., Hawn, T.R., Yi, E.C., Goodlett, D.R., Eng, J.K., Akira, S., Underhill, D.M., Aderem, A. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-1103, 2001.   DOI   ScienceOn
5 Alexopoulou, L., Holt, A.C., Medzhitov, R., Flavell, R.A. Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature. 413(6857):732-738, 2001.   DOI   ScienceOn
6 Lee, J., Mo, J.H., Katakura, K., Alkalay, I., Rucker, A.N., Liu, Y.T., Lee, H.K., Shen, C., Cojocaru, G., Shenouda, S., Kagnoff, M., Eckmann, L., Ben-Neriah, Y., Raz, E. Maintenance of colonic homeostasis by distinctive apical TLR9 signalling in intestinal epithelial cells. Nature Cell Biology. 8(12):1327-1336, 2006.   DOI   ScienceOn
7 Takeda, K., Akira, S. Toll-like receptors in innate immunity. International Immunology. 17(1):1-14, 2005.
8 Rhee, S.H., Hwang, D. Murine Toll-like receptor 4 confers lipopolysaccharide responses as determined by activation of NF kappa B and expression of the induction cyclooxygenase. Journal of Biological Chemistry. 275(44):34035-34040, 2000.   DOI   ScienceOn
9 Fitzgerald, K.A., McWhirter, S.M., Faia, K.L., Rowe, D.C., Latz, E., Golenbock, D.T., Coyle, A.J., Liao, S.M., Maniatis, T. IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway. Nature Immunology. 4(5):491-496, 2003.   DOI   ScienceOn
10 Curtiss, L.K., Tobias, P.S. Emerging role of Toll-like receptors in atherosclerosis. Journal of Lipid Research. Supplment(50):S340-S345, 2009.
11 Ariga, T., Hamano, M. Radical scavenging action and its mode in procyanidins B-1 and B-3 from azuki beans to peroxyl radicals. Agricultural Biology and Chemistry. 54: 2499-2504, 1990.   DOI
12 Zipris, D., Lien, E., Xie, J.X., Greiner, D.L., Mordes, J.P., Rossini, A.A. TLR activation synergizes with Kilham rat virus infection to induce diabetes in BBDR rats. Journal of Immunology. 174(1):131-142, 2005.
13 Cook, D.N., Pisetsky, D.S., Schwartz, D.A. Toll-like receptors in the pathogenesis of human disease. Nature Immunology. 5(10):975-979, 2004.   DOI   ScienceOn
14 Trompette, A., Divanovic, S., Visintin, A., Blanchard, C., Hegde, R.S., Madan, R., Thorne, P.S., Wills-Karp, M., Gioannini, T.L., Weiss, J.P., Karp, C.L. Allergenicity resulting from functional mimicry of a Toll-like receptor complex protein. Nature. 457(7229):585-588, 2009.   DOI   ScienceOn
15 Itoh, T., Umekawa, H., Furuichi, Y. Potential ability of hot water adzuki (Vigna angularis) extracts to inhibit the adhesion, invasion and metastasis of murine B16 melanoma cells. Bioscience, Biotechnology, and Biochemistry. 69(3):448-454, 2005.   DOI   ScienceOn
16 Itoh, T., Furuichi, Y. Lowering serum cholesterol level by feeding a 40% ethanol-eluted fraction from HP-20 resin treated with hot water extract of adzuki beans (Vigna angularis) to rats fed a high-fat cholesterol diet. Nutrition. 25(3):318-321, 2009.   DOI   ScienceOn
17 Mukai, Y., Sato, S. Polyphenol-containing azuki bean (Vigna angularis) extract attenuates blood pressure elevation and modulates nitric oxide synthase and caveolin-1 expressions in rats with hypertension. Nutrition, Metabolism and Cardiovascular Diseases. 19(7):491-497, 2009.   DOI   ScienceOn
18 Kawai, T., Akira, S. Signaling to NF-kB by Toll-like receptors. Trends in Molecular Medicine. 13(11):460-469, 2007.   DOI   ScienceOn
19 Facchini, F.S., Saylor, K.L. A low-iron-available, polyphenol-enriched, carbohydrate-restricted diet to slow progression of diabetic nephropathy. Diabetes. 52(5):1204-1209, 2003.   DOI   ScienceOn
20 Mukai, Y., Sato, S. Polyphenol-containing azuki bean (Vigna angularis) seed coats attenuate vascular oxidative stress and inflammation in spontaneously hypertensive rats. The Journal of Nutritional Biochemistry. 22(1):16-21, 2011.   DOI   ScienceOn
21 Hoffman, E.S., Smith, R.E., Renaud, R.C. Jr. From the analyst's couch: TLR-targeted therapeutics. Nature review drug discovery. 4(11):879-880, 2005.   DOI   ScienceOn
22 O'Neill, L.A., Bryant, C.E., Doyle, S.L. Therapeutic targeting of Toll-like receptors for infectious and inflammatory diseases and cancer. Pharmacologicals reviews. 61(2):177-197, 2009.
23 李時珍. 本草綱目. 北京. 人民衛生出版社, pp 833-837, 1456-1457, 508-512, 1982.
24 Ward, J.R., Francis, S.E., Marsden, L., Suddason, T., Lord, G.M., Dower, S.K., Crossman, D.C., Sabroe, I. A central role for monocytes in Toll-like receptor-mediated activation of the vasculature. Immunology. 128(1):58-68, 2009.   DOI   ScienceOn
25 Farina, C., Theil, D., Semlinger, B., Hohlfeld, R., Meinl, E. Distinct responses of monocytes to Toll-like receptor ligands and inflammatory cytokines. International Immunology. 16(6):799-809, 2004.   DOI   ScienceOn
26 Charo, I.F., Ransohoff, R.M. The many roles of chemokines and chemokine receptors in inflammation. The New England journal of medicine. 354: 610-621, 2006.   DOI   ScienceOn
27 Sato, S., Mukai, Y., Yamate, J., Kato, J., Kurasaki, M., Hatai, A., Sagai, M. Effect of polyphenol-containing azuki bean (Vigna angularis) extract on blood pressure elevation and macrophage infiltration in the heart and kidney of spontaneously hypertensive rats. Clinical and Experimental Pharmacology and Physiology. 35(1):43-49, 2008.   DOI   ScienceOn
28 Bendtzen, K. Interleukin 1, interleukin 6 and tumor necrosis factor in infection, inflammation and immunity. Immunology Letters. 19(3):183-191, 1988.   DOI   ScienceOn