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Anti-inflammatory Effect of Red Ginseng through Regulation of MAPK in Lipopolysaccharide-stimulated RAW264.7  

Shin, Ji-Su (School of Koran Medicine, Pusan National University)
Kim, Jong-Myoung (College of Fisheries Sciences, Pukyong National University)
An, Won-Gun (School of Koran Medicine, Pusan National University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.26, no.3, 2012 , pp. 293-300 More about this Journal
Abstract
Inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) are important inflammatory mediators implicated in pathogenesis of inflammation and certain types of human cancers. The present study was designed to determine whether Red Ginseng (RG) could modulate $I{\kappa}B$-kinase, iNOS and COX-2 gene expression and immune responses in RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS). RG extract suppressed the expression of LPS-induced $I{\kappa}B$, iNOS, COX-2, and immune responses in a dose-dependent manner. It also showed an anti-inflammatory effect by inhibiting NF-${\kappa}B$ immune response induced by LPS treatment. Inhibitory effect of RG on LPS-induced inflammation was mediated by suppressed phosphorylation of ERK, JNK and p38 through the regulation of the mitogen-activated protein kinase (MAPK) pathway leading to a decreased production of NO, iNOS, COX-2 and NF-${\kappa}B$. The results implied the role of RG as an inflammation regulator and its possible application for curing inflammatory diseases.
Keywords
Red Ginseng; RAW264.7 macrophages; $I{\kappa}B$; NF-${\kappa}B$; iNOS; MAPK; inflammatory diseases;
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Times Cited By KSCI : 3  (Citation Analysis)
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