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Anti-inflmmatory Effects of Scutellaria baicalensis Georgi Water Extract in the THP-1 Cells Activated by Advanced Glycation End Products  

Park, Pyeong-Beom (Department of Pathology, College of Oriental Medicine, Woosuk University)
Kim, Min-Jun (Department of Pathology, College of Oriental Medicine, Woosuk University)
Shin, Kyoung-Ho (Department of Pathology, College of Oriental Medicine, Woosuk University)
Lee, Kwang-Gyu (Department of Pathology, College of Oriental Medicine, Woosuk University)
Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Lee, Sang-Ryong (Department of Meridian & Acupoint, College of Oriental Medicine, Woosuk University)
Ha, Ki-Tae (Division of Applied Medicine, School of Korean Medicine, Pusan National University)
Jeong, Han-Sol (Division of Applied Medicine, School of Korean Medicine, Pusan National University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.26, no.3, 2012 , pp. 273-280 More about this Journal
Abstract
Scutellaria baicalensis Georgi, which has been known to be able to clear away heat and remove dampness, was used for febrile disease. It is now clear that Advanced glycation end products (AGEs) play major roles in the pathogenesis of diabetic complications such as atherosclerosis. In this study, we examined whether Scutellaria baicalensis Georgi suppress the AGE mediated inflammatory responses in the THP-1 cells. AGE treatment increased the gene expression of pro-inflammatory cytokines such as tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$), monocyte chemotactic protein-1 (MCP-1) and cyclooxygenase-2 (COX-2). Reverse transcriptase-polymerase chain reaction and Western blot analysis revealed that S. baicalensis had inhibitory effects on the expression of pro-inflammatory genes and protein levels in AGE-treated THP-1 cells. S. baicalensis had also reduced the production of ROS in the AGE-treated THP-1 cells. These results suggest that S. baicalensis has inhibitory effects for the development of diabetic vascular complication.
Keywords
advanced glycation end products; AGE; scutellaria baicalensis georgi; THP-1 cells;
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