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Different Level of Plasma Free Hemoglobin between Qi-deficiency and Fire Heat among Korean Stoke Subjects  

Lim, Ji-Hye (Brain Disease Research Center, Korea Institute of Oriental Medicine)
Ko, Mi-Mi (Brain Disease Research Center, Korea Institute of Oriental Medicine)
Lee, Jung-Sup (National Rehabilitation Center)
Lee, Myeong-Soo (Brain Disease Research Center, Korea Institute of Oriental Medicine)
Cha, Min-Ho (Brain Disease Research Center, Korea Institute of Oriental Medicine)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.25, no.4, 2011 , pp. 697-701 More about this Journal
Abstract
The purpose of this study was to fine proteins, which have significantly different level in plasma between Qi-deficiency and Fire-heat group of Korean Oriental Stroke pattern identification (PI) among Korean stroke patients. Eighteen stroke patients with Qi-deficiency and forty nine patients with Fire-heat, which had critical syndrome of each PI, were participated in this study. Plasma protein pattern were analyzed by SELDI-TOF MS using Q10 strong anion exchange chip and Mass spectral data (m/z) statistically determined. The expression level of proteins, which were different between Qi-deficiency and Fire-heat in the results by SELDI-TOF MS, were confirmed by western blot. As a result of analyzing plasma protein by SELDI-TOF MS, six protein peaks were significantly higher in Fire-heat group than Qi-deficiency group. Two peaks among of them, M15003 and M15745, were respectively identified as hemoglobin alpha and beta in previous study. Expression level of plasma free hemoglobin of Fire-heat group was also confirmed higher in Fire-heat group than in Qi-deficiency group. These findings suggest that plasma free hemoglobin is a candidate for discriminating Qi-deficiency and Fire-heat group according to pattern identification (PI) of stroke.
Keywords
Qi-deficiency; Fire-heat; pattern identification/syndrome differentiation; hemoglobin; SELDI- TOF MS; Stroke;
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