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Neuroprotective Effect of the Acid Hydrolysis Fraction of the Roots of Polygala Tenuifolia  

Lee, Dong-Sung (College of Pharmacy, Wonkwang University)
Choi, Hyun-Gyu (College of Pharmacy, Wonkwang University)
Li, Bin (College of Pharmacy, Wonkwang University)
Kim, Kyung-Su (College of Pharmacy, Wonkwang University)
Kim, Soon-Ai (College of Pharmacy, Wonkwang University)
Chon, Seung-Ki (College of Pharmacy, Wonkwang University)
Rho, Jung-Mi (College of Pharmacy, Wonkwang University)
Kim, Ki-Mo (College of Pharmacy, Wonkwang University)
Han, Jong-Hyun (Department of Pharmacology, College of Oriental Medicine, Wonkwang University)
Jeong, Gil-Saeng (College of Pharmacy, Keimyung University)
Kim, Youn-Chul (College of Pharmacy, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.25, no.4, 2011 , pp. 628-634 More about this Journal
Abstract
The roots of Polygala tenuifolia Willd. is a well-known traditional medicine used as expectorant, tonic, tranquilizer in Asia including China and Korea. And also have been used to treat amnesia, neurasthenia, palpitation, insomnia, and disorientation. Glutamate-induced oxidative injury contributes to neuronal degeneration in many central nervous system (CNS) diseases, such as Parkinson's disease, Alzheimer's disease, epilepsy and ischemia. Inducible heme oxygenase (HO)-1 acts against oxidants that are thought to play a role in the pathogenesis of these diseases. NNMBS269, acid hydrolysis EtOAc fraction of the P. tenuifolia showed dominant neuroprotective effects on glutamate-induced neurotoxicity in mouse hippocampal HT22 cells while general EtOAc fraction of the P. tenuifolia (NNMBS268) not shown. NNMBS269 induced the expression of HO-1 protein that has been proposed to play an important cellular defense role against oxidant injury. In addition increased HO activity. In mouse hippocampal HT22 cells, NNMBS269 makes the nuclear accumulation of nuclear factor E2-related factor 2 (Nrf2). In conclusion, acid hydrolysis EtOAc fraction the P. enuifolia. (NNMBS269) significantly protect glutamate-induced oxidative damage by induction of HO-1 via Nrf2 translocation in mouse hippocampal HT22 cells.
Keywords
polygala tenuifolia; heme oxygenase-1; HT22; glutamate; neuroprotective effect;
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