Browse > Article

Hepatoprotective Effect of Bark of Phellodendron amurense RUPR. on Liver Damage Induced by Carbon Tetrachloride  

Kwak, Chang-Geun (Department of Pathology, College of Oriental Medicine, Dongguk University)
Kim, Jae-Eun (Department of Pathology, College of Oriental Medicine, Dongguk University)
Choi, Dall-Yeong (Department of Pathology, College of Oriental Medicine, Dongguk University)
Jeong, Han-Sol (Division of Applied Medicine, School of Korean Medicine, Pusan National University)
Shin, Sang-Woo (Division of Applied Medicine, School of Korean Medicine, Pusan National University)
Joo, Myoung-Su (Division of Applied Medicine, School of Korean Medicine, Pusan National University)
Ha, Ki-Tae (Division of Applied Medicine, School of Korean Medicine, Pusan National University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.25, no.4, 2011 , pp. 620-627 More about this Journal
Abstract
We evaluated the hepatoprotective activity of crude hot-water extracts of the traditional Korean medicinal herb, Phellodendri Cortex (PC; Bark of Phellodendron amurense RUPR.), in an experimental model of hepatic damage induced by carbon tetrachloride ($CCl_4$). The serum marker of liver damage, sGOT, sGPT and sALP, were significantly decreased in the liver of the PC treated rats, compared with that of $CCl_4$ treated group. The histological observation of liver section of rats, showed the same protective effect of PC treatment. And the protective activity of PC was more significant in the post-treated group than pre-treated group. The significant decrease of malodialdehyde and increase of glutathion, catalase activity were observed in the liver homogenate of PC treated rats. Based on these findings, it is suggested that PC has potent hepatoprotective effects and the mechanism of the protection may be related to antioxidation pathways.
Keywords
phellodendri cortex; carbon tetrachloride; hepatoprotection; antioxidation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ohkawa, H., Ohishi, N., Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95(2):351-358, 1979.   DOI   ScienceOn
2 Ellman, G.L. Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82: 70-77, 1959.   DOI   ScienceOn
3 Aebi, H. Catalase in vitro. Methods Enzymol. 105: 121-126, 1984.
4 Poli, G. Liver damage due to free radicals. Br Med Bull. 49(3):604-620, 1993.   DOI
5 Hong, S.J., Fong, J.C., Hwang, J.H. Effects of crude drugs on glucose uptake in 3T3-L1 adipocytes. Kaohsiung J. Med. Sci. 16(9):445-451, 2000.
6 Kong, L.D., Yang, C., QuiXi, Wu, H.P., Ye, D.J. Effects of different processing products of Cortex Phellodendri on scavenging oxygen free radicals and anti-lipid peroxidation. Zhongguo Zhong Yao Za Zhi. 26(4):245-248, 2001.
7 Park, J.I., Shim, J.K., Do, J.W., Kim, S.Y., Seo, E.K., Kwon, H.J., Lee, T.K., Kim, J.K., Choi, D.Y., Kim, C.H. Immune-stimulating properties of polysaccharides from Phellodendri cortex (Hwangbek). Glycoconj J. 16(3):247-252, 1999.   DOI   ScienceOn
8 Reitman, S. and Frankel, S. A colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases, Am. J. Clin. Pathol. 28: 58-63, 1957.
9 Petkova, J., Popova, N., Kemileva, Z. Changes of enzyme activity in some organs following thymectomy. Agressologie. 14(5):323-326, 1973.
10 김호철. 한약약리학. 서울, 집문당, pp 138-140, 2001.
11 Mori, H., Fuchigami, M., Inoue, N., Nagai, H., Koda, A., Nishioka, I., Meguro, K. Principle of the bark of Phellodendron amurense to suppress the cellular immune response: effect of phellodendrine on cellular and humoral immune responses. Planta Med. 61(1):45-49, 1995.   DOI
12 전국한의과대학본초학교수 공편. 본초학. 서울, 영림사, pp 182-183, 2000.
13 Hong, S.J., Fong, J.C., Hwang, J.H. Effects of crude drugs on lipolysis in differentiated 3T3-L1 adipocytes. Kaohsiung J. Med. Sci. 18(4):157-163, 2002.
14 Fernandez, G., Villarruel, M.C., de Toranzo, E.G., Castro, J.A. Covalent binding of carbon tetrachloride metabolites to the heme moiety of cytochrome P-450 and its degradation products. Res. Commun. Chem. Pathol. Pharmacol. 35(2):283-290, 1982.
15 Tomasi, A., Albano, E., Banni, S., Botti, B., Corongiu, F., Dessi, M.A., Iannone, A., Vannini, V., Dianzani, M.U. Free-radical metabolism of carbon tetrachloride in rat liver mitochondria. A study of the mechanism of activation. Biochem. J. 246(2):313-317, 1987.   DOI
16 Le Page, R.N., Cheeseman, K.H., Osman, N., Slater, T.F. Lipid peroxidation in purified plasma membrane fractions of rat liver in relation to the hepatoxicity of carbon tetrachloride. Cell. Biochem. Funct. 6(2):87-99, 1988.   DOI   ScienceOn
17 서혜진. 사염화탄소의 반복투여가 백서 간장에 미치는 병리 조직학적 연구. 영남대학교 대학원 석사학위논문, 1987.
18 章眞如. 肝膽論, 湖北, 湖北科學技術出版社, pp 3-9, 1986.
19 전국한의과대학 병리학교실 공저. 한방병리학. 서울, 一中社, pp 266-287, 2002.
20 조영주, 김성훈. 지골피 EA분획의 $CCL_{4}$ 손상간에 대한 보호 작용. 대한동의병리학회지 11(2):63-71, 1997.
21 匡調元 主編. 中醫實驗病理學. 上海, 上海科學普及出版社, pp 128-135, 1995.
22 Poli, G., Chiarpotto, E., Albano, E., Cottalasso, D., Nanni, G., Marinari, U.M., Bassi, A.M., Dianzani, M.U. Carbon tetrachloride-induced inhibition of hepatocyte lipoprotein secretion: functional impairment of Golgi apparatus in the early phases of such injury. Life Sci. 36(6):533-539, 1985.   DOI   ScienceOn
23 Chaudiere, J. and Ferrari-Iliou, R. Intracellular antioxidants: from chemical to biochemical mechanisms. Food. Chem. Toxicol. 37(9-10):949-962, 1999.   DOI   ScienceOn
24 Deisseroth, A. and Dounce, A.L. Catalase: Physicial and Chemical Properties, Mechanism of Catalysis, and Physiological Role, Physiol. Rev. 50: 319-375, 1970.   DOI
25 Melen, K., Hultberg, B., Hagerstrand, I., Isaksson, A., Joelsson, B., Bengmark, S. Lysosomal enzymes in plasma, liver and spleen from rats with carbon tetrachloride-induced liver cirrhosis. Enzyme. 33(2):84-88, 1985.   DOI
26 Esterbauer, H., Schaur. R.J., Zollner. H. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radical Biology & Medicine. 11: 81-128, 1991.   DOI   ScienceOn
27 Anderson, M.E. Enzymatic and chemical methods for the determination of glutathione; In Glutathione: chemical, biochemical and medical aspects, Vol.A, Dolphin D., Poulson R. and Avramovic O. Eds., John WILEY and Sons, pp 339-365, 1989.
28 Clawson, G.A., Sesno, J., Milam, K., Wang, Y.F., Gabriel, C. The hepatocyte protein synthesis defect induced by galactosamine involves hypomethylation of ribosomal RNA. Hepatology 11(3):428-434, 1990.   DOI
29 Watanabe, A., Akamatsu, K., Takesue, A., Taketa, K. Dysregulation of protein synthesis in injured liver. A comparative study on microsomal and cytosole enzyme activities, microsomal lipoperoxidation and polysomal pattern in D-galactosamine and carbon tetrachloride-injured livers. Enzyme. 23(5):320-327, 1978.   DOI
30 Biasi, F., Albano, E., Chiarpotto, E., Corongiu, F.P., Pronzato, M.A., Marinari, U.M., Parola, M., Dianzani, M.U., Poli, G. In vivo and in vitro evidence concerning the role of lipid peroxidation in the mechanism of hepatocyte death due to carbon tetrachloride. Cell Biochem Funct. 9(2):111-118, 1991.   DOI   ScienceOn
31 Weddle, C.C., Hornbrook, K.R. and McCay, P.B. Lipid peroxidation and alteration of membrane lipids in isolated hepatocytes exposed to carbon tetrachloride. J. Biol. Chem. 251(16):4973-4978, 1976.
32 Chenoweth, M.B. and Hake, C.L. The smaller halogenated aliphatic hydrocarbons. Ann. Rev. Pharmac. 2: 363-398, 1962.   DOI   ScienceOn
33 Kamokawa, A., Ohta, S., Tatsugi, A., Kumasaka, M. and Shinoda, M. Experimental Production of Various Types of Cholestasis and the Effects of Cystemine. YAKUGAKU ZASSHI. 106(8):709-714, 1986.   DOI
34 Noguchi, T., Fong, K. L., Lai, E. K., Olson, L. and McCay, P.B. Selective early loss of polypeptides in liver microsomes of $CCL_{4}$-treated rats. Relationship to cytochrome P-450 content. Biochem. Pharmacol. 31(5):609-614, 1982.   DOI   ScienceOn