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Action Mechanism of Anticonvulsive Effect of Nelumbo Nucifera in Pentylenetetrazole-induced Animal Models  

Kim, Sung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University)
Choi, Jong-Won (College of Pharmacy, Kyungsung University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.25, no.4, 2011 , pp. 614-619 More about this Journal
Abstract
Nelumbo nucifera(NN) is a oriental medicinal herb which has been used traditionally for the treatment of antidiarrhea, sedative action and various brain diseases including convulsion and epilepsy. In order to examine the mechanism of anticonvulsive effect, we treated the methanol extract of NN(100, 200 mg/kg, P.0) to the sleeping time and pentylenetetrazole(PTZ)-induced convulsive mice. The methanol extract of NN prolonged sleep time by pentobarbital. Methanol extracts of NN were not effected the concentration of GABA and GABA-T activity in the brain of PTZ-induced mice. Methanol extracts of NN significantly inhibited the convulsion state as well as the level of lipid peroxidation in the brain. The butanol and dichloromethane fraction of methanol extracts among the others effectively inhibited in vitro lipid peroxidation dose dependently($5.0{\times}10^{-6}\sim2.0{\times}10^{-5}\;g/ml$). These results suggest that the anticonvulsive effect of NN is possibly due to the antioxidative effects of the free radical formation at brain for the PTZ-induced convulsion if it were by due to generating system.
Keywords
Nelumbo nucifera; sleeping time; anticonvulsant; antioxidant;
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1 Cohen, G.M., Doherty, M.A. Free radical mediated cell toxicity by redox cycling chemicals. Br J Cancer Suppl. 8: 46-52, 1987.
2 Behl, C., Davis, J.B.,, Lesley, R., Schubert, D. Hydrogen peroxide mediates amyloid $\beta$ protein toxicity, Cell 77(6):817-827, 1994.   DOI   ScienceOn
3 Bejma, J., Ji, L.L. Aging and acute exercise enhance free radical generation in rat skeletal muscle. J Applied Physiology. 87(1):465-470, 1999.   DOI
4 Valko, M., Rhodesb, C.J., Mocola, J., Izakovica, M., Mazura, M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biol Interactions 160(1):1-40, 2006.   DOI   ScienceOn
5 Jomova, K., Valko, M. Advances in metal-induced oxidative stress and human disease. Toxicol. 283(2):65-87, 2011.   DOI   ScienceOn
6 Hall, E.D., Braughler, M.J. The role of oxygen radical-induced lipid peroxidation in acute CNS trauma. Oxygen radicals and tissue injury (Ed Halliwell B.). FASEB Bethesda MD. p 92, 1988.
7 Carney, J.M., Oliver, C.N., Landum, R.W., Cheng, M.S., Wu, J.F., Floyd, R.A. Reversal of age-related changes in brain protein oxidation decrease in enzyme activity. Proc Natl Acad Sci. 88: 3633-3636, 1991.   DOI   ScienceOn
8 Coyle, J.T., Puttfarcken, P. Oxygen stress, glutamate and neurodegenerative disorders. Science 262(5134):689-695, 1993.   DOI
9 Schwarcz, R., Bennett, J.P. Jr, Coyle, J.T. Inhibitors of GABA metabolism: implications for Huntington's disease. Ann Neurol. 2(4):299-303, 1977.   DOI   ScienceOn
10 Holdiness, M.R. Chromatographic analysis of glutamic acid decarboxylase in biological samples. J Chromatography 277: 1-24, 1984.
11 Dulac, O., Arthuis, M. Open trials with vaproate in epilepsy. Epilepsia 25: S23, 1984.   DOI
12 Elwes, R.D.C., Johnson, A.L., Shorvon, S.D., Reynolds, E.H. The prognosis for seizure control in newly diagnosed epilepsy. New Engl J Med. 311: 944-947, 1984.   DOI   ScienceOn
13 Callaghan, N., Goggin, T. Adjunctive therapy in resistant epilepsy. Epilepsia 29: S29, 1988.   DOI
14 Roussinov, K.S., Azarova, M.B., Atanassova-shopova, S. .On certain relationships between gamma-aminobutyric acid (GABA) and adrenergic mechanisms in convulsive-seizure reaction. Acta Physiol Pharmacol Bulg. 2(3):69-76, 1976.
15 Vogel, H.G. Drug discovery and evalution-pharmacological assays. Springer Press. pp 422-423, 2007.
16 Allen, I.C., Griffthis, R. Reversed-phase high performance liquid chromatographic method for determination of brain glutamate carboxylase suitable for use in kinetic studies. J Chromatography 336(2):385-391, 1984.   DOI
17 Cupello, A., Larsson, S., Palm, A., Rapallino, M.V. A microelectrophoretic method for the evaluation of GABA transaminase activity. Neurochemical Research. 13(7):601-603, 1988.   DOI   ScienceOn
18 Ohkawa, H., Ohishi, N., Yaki, K. Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction. Anal Biochem. 95(2):351-358, 1979.   DOI   ScienceOn
19 Halliwell, B., Gutteridge, M.C., Aruoma, O.I. The deoxyribose method: A simple "test-tube" assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem. 165(1):215-219, 1987.   DOI   ScienceOn
20 Lowry, O.H., Rosebrough, N.J., Farr, A.L., Rendall, R.J. Protein measurement with folin phenol reagent. J Biol Chem. 193(1):265-275, 1951.
21 Mukherjee, P.K., Das, J., Balasubramanian, R., Saha, K., Pal, M., Saha, B.P. Antidiarrhoeal evaluation of Nelumbo nuficera rhizome extract. Indian J Pharmacol. 27: 262-364, 1995.
22 Mukherjee, P.K., Giri, S.N., Saha, K., Pal, M., Saha, B.P. Antifungal screening of Nelumbo nuficera (Nymphaeaceae) rhizome extract. Indian J Pharmacol. 35: 327-330, 1995.
23 Mukherjee, P.K., Balasubramanian, R., Saha, K., Pal, M., Saha, B.P. Antibacterial efficiency of Nelumbo nuficera (Nymphaeaceae) rhizome extract. Indian Drugs 32: 274-276, 1995.
24 Hu, M., Skibsted, L.H. Antioxidative capacity of rhizome extract and rhizome knot extract of edible lotus (Nelumbo nuficera). Food Chemistry 76: 327-333, 2002.   DOI   ScienceOn
25 Seo, J.H., Choi, Y.H., Yoo, M.Y., Hong, K.S., Lee, B.H., Yon, G.H., Kim, Y.S., Kim, Y.K., Ryu, S.Y. Isolation of Dihydrophaseic Acid from Seed Extract of Nelumbo nucifera, Korean J Pharmacognosy 37: 290-293, 2006.
26 Song, G.S., Ahn, B.Y., Lee, K.S., Maeng, I.K., Choi, D.S. Effect of hot water extracts from medical plants on the mutagenicity of indirect mutagens. J Food Sci Technol. 29: 1288-1294, 1997.
27 Ma, Y., Ma, H., Eun, J.S., Nam, S.Y., Kim, Y.B., Hong, J.T., Lee, M.K., Oh, K.W. Methanol extract of Longanae Arillus augments modification of GABAergic system. J Ethnopharmacol. 122: 245-250, 2009.   DOI   ScienceOn
28 Callenbach, P.M., Westendorp, R.G., Geerts, A.T., Arts, W.F., Peeters, E.A., van Donselaar, C.A., Peters, A.C., Stroink, H., Brouwer, O.F. Mortality Risk in Children with Epilepsy: The Dutch Study of Epilepsy in Childhood, Pediatrics 107: 1259-1263, 2001.   DOI   ScienceOn
29 Reuber, M., Fernández, G., Bauer, J., Helmstaedter, C., Elger, C.E. Diagnostic delay in psychogenic nonepileptic seizures. Neurology 58(3):493-495, 2002.   DOI   ScienceOn
30 Baughman, R.W., Gilbert, C.D. Aspartate and glutamate as possible neurotransmitters in the visual cortex. J Neuroscience 1(4):427-439, 1981.   DOI
31 Aoki, E., Semba, R., Kato, K., Kashiwamata, S. Purification of specific antibody against aspartate and immunocytochemical localization of aspartergic neurons in the rat brain. Neuroscience 21(3):755-765, 1987.   DOI   ScienceOn
32 Halliwell, B., Reactive oxygen species and the central nerve system. J Neurochem. 59: 1609-1623, 1992.   DOI
33 Coyle, J.T., Puttfarcken, P. Oxidative stress, glutamate, and neurodegenerative disorders, Science 262(5134):689-695, 1993.   DOI
34 Mukherjee, P.K., Pal, S.R., Saha, K., Saha, B.P. Hypoglycemic activity of Nelumbo nuficera rhizome (methanolic extract) in streptozotocin induced diabetic rats. Phytotherapy Research. 9: 522-524, 1995.   DOI   ScienceOn
35 Shackleton, D.P., Westendorp, R.G.J., Kasteleijn-Nolst Trenite, D.G.A., Vandenbroucke, J.P. Mortality in patients with epilepsy: 40 years of followup in a Dutch cohort study. J. Neurol. Neurosurg. Psychiatry 66: 636-640, 1999.   DOI   ScienceOn