Browse > Article

Effects of Carthami Flos on Interstitial Cells of Cajal in the Gastrointestinal Tract  

Song, Ho-Joon (Division of Longevity and Biofunctional Medicine, Pusan National University)
Kim, Jung-A (Division of Longevity and Biofunctional Medicine, Pusan National University)
Han, Song-Ee (Division of Longevity and Biofunctional Medicine, Pusan National University)
Kim, Hyung-Woo (Division of Pharmacology School of Korean Medicine, Pusan National University)
Chae, Han (Division of Longevity and Biofunctional Medicine, Pusan National University)
Kim, Byung-Joo (Division of Longevity and Biofunctional Medicine, Pusan National University)
Kwon, Young-Kyu (Division of Longevity and Biofunctional Medicine, Pusan National University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.25, no.4, 2011 , pp. 603-607 More about this Journal
Abstract
The purpose of this study is to investigate the effects of Carthami Flos on interstitial cells of Cajal in the gastrointestinal tract. Many regions of the tunica muscularis of the gastrointestinal (GI) tract display spontaneous contraction. These spontaneous contractions are mediated by periodic generation of electrical slow waves. Recent studies have shown that the interstitial cells of Cajal (ICCs) act as pacemakers and conductors of electrical slow waves in gastrointestinal smooth muscles. We investigated the cytotoxicity activity, antioxidant activity, and pacemaking activity. The cytotoxicity activity was measured by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Antioxidant activities were determined by DPPH (1.1-diphenyl-2-picrylhydrazyl) radical scavenging capacity assay and DCFH-DA (2,7-dichlorofluorescein diacetate) method. The effects of Carthami Flos on the pacemaker potentials in cultured ICCs from murine small intestine were investigated by using whole-cell patch-clamp techniques at $30^{\circ}C$. The addition of Carthami Flos (5, 10, $30{\mu}g$/ml) depolarized the resting membrane potentials in a concentration dependent manner. These results suggest that the GI tract can be targets for Carthami Flos, and their interaction can affect intestinal motility.
Keywords
Carthami Flos; Interstitial cells of Cajal; gastrointestinal tract;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Koh, S.D., Sanders, K.M., Ward, S.M. Spontaneous electrical rhythmicity in cultured interstitial cells of cajal from the murine small intestine. J. Physiol. 513: 203-213, 1998.   DOI   ScienceOn
2 Huizinga, J.D., Thuneberg, L., Klüppel, M., Malysz, J., Mikkelsen, H.B., Bernstein, A. W/kit gene required for interstitial cells of Cajal and for intestinal pacemaker activity. Nature 373: 347-349, 1995.   DOI   ScienceOn
3 Cox, C., Mann, J., Sutherland, W., Chisholm, A., Skeaff, M. Effects of coconut oil, butter, and safflower oil on lipids and lipoproteins in persons with moderately elevated cholesterol levels. J. Lipid Res. 36: 1787-1795, 1995.
4 Iwata, T., Ohya, K., Takehisa, F., Tsutsumi, K., Furukawa, Y., Kimura, S. The effect of dietary safflower phospholipid on sterols in gastrointestinal tract of rats fed a hypercholesterolemic diet. J. Nutr. Sci. Vitaminol. 38: 615-622, 1992.   DOI   ScienceOn
5 최영주, 최상욱. 홍화(Carthamus tinctorius L.)씨의 sterol 및 phytoestrogen 분석. Korean Journal of Life Science 13(4):529-534, 2003.   DOI
6 한국생약교수협의회. 본초학. 사단법인 약사회. pp 553-555, 1995.
7 이인우, 최진규. 홍화씨 건강법. 태일출판사, pp 45-70, 1998.
8 Tokutomi, N., Maeda, H., Tokutomi, Y., Sato, D., Sugita, M., Nishikawa, S., Nishikawa, S., Nakao, J., Imamura, T., Nishi, K. Rhythmic Cl- current and physiological roles of the intestinal c-kit positive cells. Pflugers Arch. 431: 169-177, 1995.   DOI   ScienceOn
9 Ward, S.M., Morris, G., Reese, L., Wang, X.Y., Sanders, K.M. Interstitial cells of Cajal mediate enteric inhibitory neurotransmission in the lower esophageal and pyloric sphincters. Gastroenterology 115: 314-329, 1998.   DOI   ScienceOn
10 Cajal, S.R. Sur les ganglions et plexus nerveux de I'intestin. CR Soc Biol (Paris) 45: 217-223, 1893.
11 Sanders, K.M. A case for interstitial cells of Cajal as pacemakers and mediators of neurotransmission in the gastrointestinal tract. Gastroenterology. 111: 492-515, 1996.   DOI   ScienceOn
12 Heo J. Dong-I Bogam 5. 3th eds., In: Safflower. Yekang Publishers, Seoul, Korea. pp 2763-2764, 1986.
13 Kee, C.H. The Pharmacology of Chinese Herbs. CRC press, pp 249-250, 1993.
14 Kim, M.N., Kim, K.H. Researches for analgesic and hepatoprotective action of Carthami Flos. Pusan Bull. Pharm. Sci. 26: 32-36, 1992.
15 Cleary, M.P., Phillips, F.C., Morton, R.A. Genotype and diet effects in lean and obese Zucker rats fed either safflower or coconut oil diets. Proc. Soc. Exp. Biol. Mec. 220: 153-161, 1999.   DOI   ScienceOn
16 Farrugia, G. Ionic conductances in gastrointestinal smooth muscles and interstitial cells of Cajal. Annu. Rev. Physiol. 61: 45-84, 1999.   DOI   ScienceOn
17 김미진, 김자영, 최상원, 홍진태, 윤경섭. Anti-wrinkle Effect of Safflower (Carthamus tinctorius) Seed Extract (II). 대한화장품학회지 30(4):449-456, 2004.
18 Szurszewski, J.H. Electrical basis for gastrointestinal motility. In Physiology of the gastrointestinal tract, 2nd edn, ed. Johnson LR, Raven Press, New York, pp 383-422, 1987.
19 Tomita, T. Electrical activity (spikes and slow waves) in gastrointestinal smooth muscles. In Smooth Muscle: An Assessment of Current Knowledge, ed. Brading AF, Jones AW &Tomita T, Edward Arnold, London, pp 127-156, 1981.
20 김미진, 윤경섭, 김자영, 최상원, 홍진태. Anti-wrinkle Effect of Safflower (Carthamus tinctorius) Seed Extract (I). 대한화장품학회지 30(1):15-22, 2004.
21 Zhang, H.L., Nagatsu, A., Sakakibara, J. Novel antioxidants from safflower (Carthamus tinctorius L.) oil cake. Chem. Pharm. Bull. 44: 874-876, 1996.   DOI   ScienceOn
22 Zhang, H.L., Nagatsu, A., Watanabe, T., Sakakibara, J., Okuyama, H. Antioxidative compounds isolated from safflower (Carthamus tinctorius L.) oil cake. Chem. Pharm. Bull. 45(12):1910-1914, 1997.   DOI   ScienceOn
23 Takii, T., Hayashi, M., Hiroma, H., Chiba, T., Kawashima, S., Zhang, H.L., Nagatsu, A., Sakakibara, J., Onozaki, K. Serotonin derivative, N-(p-Coumaroyl)serotonin, isolated from safflower (Carthamus tinctorius L.) oil cake augments the proliferation of normal human and mouse fibroblasts in synergy with basic fibroblast growth factor (bFGF) or epidermal growth factor (EGF). J. Biochem. 125: 910-915, 1999.   DOI
24 Kim, M.J., Kim, J.Y., Choi, S.W., Hong, J.T., Yoon, K.S. Anti-wrinkle effect of safflower seed extract (I). J. Soc. Cosmet. Scientists. 30: 15-20, 2004.
25 이광현. 홍화씨로부터 추출된 세포토닌계 화합물 및 이를 함유하는 조성물. 한국공개특허 출원번호 10-2002-0008315, 2002.
26 최성희, 임성임, 장은영, 조영수. 홍화꽃 및 홍화씨의 휘발성 성분. 한국식품과학회지 14: 36-42, 2004.
27 Kim, H.J., Bae, Y.C., Park, R.W., Choi, S.W., Cho, S.H., Choi, Y.S., Lee, W.J. Bone-protecting effect of safflower seeds in ovariectomized rats. Calcif. Tissue. Int. 71(1):88-94, 2002.   DOI   ScienceOn
28 최한기. 고농서국역총서11-농정회요II (農政會要). 농촌진흥청. pp 271-277.