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Study on the Anti-inflammatory Effect of Jakyak-tang Water Extract  

Seo, Yun-Hee (Department of Preventive Medicine, College of Oriental Medicine, Wonkwang University)
Kang, Ok-Hwa (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Kwon, Dong-Yeul (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Lee, Jang-Suk (Department of Preventive Medicine, College of Oriental Medicine, Wonkwang University)
Han, Jong-Hyun (Department of Pharmacology, College of Oriental Medicine, Wonkwang University)
Lee, Ki-Nam (Department of Preventive Medicine, College of Oriental Medicine, Wonkwang University)
Chong, Myong-Soo (Department of Preventive Medicine, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.25, no.3, 2011 , pp. 503-509 More about this Journal
Abstract
Jakyaktang(芍藥湯; JYT) exhibits potent anti-inflammatory activity in widely intestinal disease, but its mechanism was undisclosed. To elucidate the molecular mechanisms of JYT on pharmacological and biochemical actions in inflammation, we examined the effect of JYT on pro-inflammatory mediators in phorbol 12-myristate 13-acetate (PMA) plus A23187-induced mast cell and lipopolysaccharide (LPS)-stimulated macrophages. The investigation focused on whether JYT inhibited pro-inflammatory cytokines such as interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) in PMA plus A23187- induced HMC-1 cells and inflammatory madiators such as nitric oxide (NO), TNF-${\alpha}$, IL-6, iNOS, COX-2 in LPS-stimulated RAW 264.7 cells. We found that JYT inhibited LPS-induced NO, TNF-${\alpha}$ and IL-6 productions as well as the expressions of iNOS and COX-2. These results suggest that JYT has inhibitory effects on mast cell-mediated and macropage-mediated inflammation.
Keywords
Jakyaktang(芍藥湯; JYT); Anti-inflammatory effect; inflammatory cytokines; inflammatory madiators; RAW 264.7 cells;
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