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Anti-inflammatory Effects of Saussurea Lappa Extracts in Murine Macrophages  

Lee, Min-Suk (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Ryu, Do-Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Kwon, Kang-Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.25, no.2, 2011 , pp. 275-279 More about this Journal
Abstract
In order to validate the use of Saussurea Lappa as an anti-inflammatory drug in the traditional Korean medicine, I have investigated the effects of water-soluble extract of Saussurea Lappa (ESL) on the production of pro-inflammatory tumor necrosis factor-alpha (TNF-${\alpha}$) in murine RAW 264.7 macrophages stimulated with the endotoxin lipopolysaccharide (LPS). The extract inhibited dose-dependently TNF-${\alpha}$ production without its cytotoxic effect on the macrophages, as measured by enzyme-linked immunosorbent assay, and significantly decreased mRNA levels of TNF-${\alpha}$, as determined using reverse transcription polymerase chain reaction. The extract also inhibited LPS-induced activation of nuclear factor-${\kappa}B$, thereby resulting in TNF-${\alpha}$ gene expression. These results suggest that ESL may have therapeutic potential in the control of inflammatory diseases mediated by activated macrophages.
Keywords
Saussurea Lappa; RAW 264.7 cells; tumor necrosis factor-${\alpha}$;
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