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Effect of Artemisiae Argi Folium Fermented with Lactobacillus Pentosus and Saccharomyces Cerevisiae on TNF-${\alpha}$ Production in RAW 264.7 and HepG2 Cells  

Kim, Youn-Sub (College of Oriental Medicine, Kyungwon University)
Park, Wan-Su (College of Oriental Medicine, Kyungwon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.24, no.6, 2010 , pp. 956-961 More about this Journal
Abstract
Tumor necrosis factor-alpha (TNF-${\alpha}$) is a major mediator of immuno-inflammatory activity. The purpose of this study is to investigate whether TNF-${\alpha}$ productions of mouse macrophage RAW 264.7 and human hepatocyte HepG2 are modulated by Artemisiae argi Folium water extract (AW), Lactobacillus pentosus-fermented Artemisiae argi Folium water extract (AFL), and Saccharomyces cerevisiae-fermented Artemisiae argi Folium water extract (AFS) for 3 h of incubation. Effect of AW on cell viability of HepG2 was also investigated. TNF-${\alpha}$ productions were measured by Enzyme-Linked Immnunosorbent Assay method and cell viability was measured by MTT assay. Both AFL and AFS significantly increased TNF-${\alpha}$ productions of RAW 264.7 at the concentration of 50, 100, and 200 ${\mu}g$/mL (p<0.05). Also, AFL and AFS significantly increased TNF-${\alpha}$ productions of HepG2 at the concentration of 50, 100, and 200 ${\mu}g$/mL (p<0.05). AW significantly increased TNF-${\alpha}$ production of HepG2 at the concentration of 100 and 200 ${\mu}g$/mL (p<0.05). AW did not show any cytotoxicity on HepG2 cells for 3 h. These results suggest that AFL, AFS, and AW have the immune-enhancing property related with its increasing effect on TNF-${\alpha}$ production of macrophage and hepatocyte.
Keywords
Artemisiae argi folium; fermentation; Saccharomyces cerevisiae; Lactobacillus pentosus; TNF-${\alpha}$;
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