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Protective Effect of Cheonjeongkibo-Dan UV-Induced Cellular Damage in Human Dermal Fibroblast  

Lee, Ghang-Tai (Coreana Cosmetics Ltd, SongPa R&D Center)
Park, Si-Jun (Coreana Cosmetics Ltd, SongPa R&D Center)
Lee, Jung-No (Coreana Cosmetics Ltd, SongPa R&D Center)
Lee, Kwang-Sik (Coreana Cosmetics Ltd, SongPa R&D Center)
Kim, Dae-Sung (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Mun, Yeun-Ja (Department of Anatomy, College of Oriental Medicine, Wonkwang University)
Lee, Kun-Kuk (Coreana Cosmetics Ltd, SongPa R&D Center)
Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.24, no.6, 2010 , pp. 950-955 More about this Journal
Abstract
In this study, we prepared CheonJeongKiBo-Dan(7 oriental medicinal plants, 7OMP: Astragalus Membranaceus root, Panax Ginseng root, Glycyrrhiza Glabra (licorice) root, Schizandra Chinensis fruit, Polygonatum Odoratum, Rehmannia Glutinosa root, Paeonia Albiflora root) by extracting them in one reactor and studied its efficacies on skin. UV irradiation has been suggested as a major cause of photoaging in skin. In order to investigate protective effects against UV-B induced cellular damage, 7OMP was extracted with 70% ethanol and dissolved in DMSO. The protective effect was detected by MTT assay, reactive oxygen species (ROS) generation, phosphorylation of ATR and p53 in human dermal fibroblast cell system after UV-B irradiation. 7OMP reduced UV-B-induced cellular damage in HDFs cells, and inhibited ROS generation. UV-B-induced toxicity accompanying ROS production and the resultant DNA damage are responsible for activation of ATR, p53 and Bad. In this study, 7OMP hampered phosphorylations of ATR and p53 in human dermal fibroblasts. Therefore, 7OMP may be protective against UV-induced skin photoaging.
Keywords
CheonJeongKiBo-Dan; photoaging; ROS; ATR; p53;
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